LOCUS       sequence-168869-        4563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PPP2R1A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PPP2R1A_WT
FEATURES             Location/Qualifiers
     source          1..4563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2442..2541)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2558..2577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2559..2577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2582..2598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2582..2598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3029..3820
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3913..4501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4402..4421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa
      661 agttggcacc atggcggcgg ccgacggcga cgactcgctg taccccatcg cggtgctcat
      721 agacgaactc cgcaatgagg acgttcagct tcgcctcaac agcatcaaga agctgtccac
      781 catcgccttg gcccttgggg ttgaaaggac ccgaagtgag cttctgcctt tccttacaga
      841 taccatctat gatgaagatg aggtcctcct ggccctggca gaacagctgg gaaccttcac
      901 taccctggtg ggaggcccag agtacgtgca ctgcctgctg ccaccgctgg agtcgctggc
      961 cacagtggag gagacagtgg tgcgggacaa ggcagtggag tccttacggg ccatctcaca
     1021 cgagcactcg ccctctgacc tggaggcgca ctttgtgccg ctagtgaagc ggctggcggg
     1081 cggcgactgg ttcacctccc gcacctcggc ctgcggcctc ttctccgtct gctacccccg
     1141 agtgtccagt gctgtgaagg cggaacttcg acagtacttc cggaacctgt gctcagatga
     1201 cacccccatg gtgcggcggg ccgcagcctc caagctgggg gagtttgcca aggtgctgga
     1261 gctggacaac gtcaagagtg agatcatccc catgttctcc aacctggcct ctgacgagca
     1321 ggactcggtg cggctgctgg cggtggaggc gtgcgtgaac atcgcccagc ttctgcccca
     1381 ggaggatctg gaggccctgg tgatgcccac tctgcgccag gccgctgaag acaagtcctg
     1441 gcgcgtccgc tacatggtgg ctgacaagtt cacagagctc cagaaagcag tggggcctga
     1501 gatcaccaag acagacctgg tccctgcctt ccagaacctg atgaaagact gtgaggccga
     1561 ggtgagggcc gcagcctccc acaaggtcaa agagttctgt gaaaacctct cagctgactg
     1621 tcgggagaat gtgatcatgt cccagatctt gccctgcatc aaggagctgg tgtccgatgc
     1681 caaccaacat gtcaagtctg ccctggcctc agtcatcatg ggtctctctc ccatcttggg
     1741 caaagacaac accatcgagc acctcttgcc cctcttcctg gctcagctga aggatgagtg
     1801 ccctgaggta cggctgaaca tcatctctaa cctggactgt gtgaacgagg tgattggcat
     1861 ccggcagctg tcccagtccc tgctccctgc cattgtggag ctggctgagg acgccaagtg
     1921 gcgggtgcgg ctggccatca ttgagtacat gcccctcctg gctggacagc tgggagtgga
     1981 gttctttgat gagaaactta actccttgtg catggcctgg cttgtggatc atgtatatgc
     2041 catccgcgag gcagccacca gcaacctgaa gaagctagtg gaaaagtttg ggaaggagtg
     2101 ggcccatgcc acaatcatcc ccaaggtctt ggccatgtcc ggagacccca actacctgca
     2161 ccgcatgact acgctcttct gcatcaatgt gctgtctgag gtctgtgggc aggacatcac
     2221 caccaagcac atgctaccca cggttctgcg catggctggg gacccggttg ccaatgtccg
     2281 cttcaatgtg gccaagtctc tgcagaagat agggcccatc ctggacaaca gcaccttgca
     2341 gagtgaagtc aagcccatcc tagagaagct gacccaggac caggatgtgg acgtcaaata
     2401 ctttgcccag gaggctctga ctgttctgtc tctcgcctga ttgccaactt tcttgtacaa
     2461 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     2521 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     2581 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     2641 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     2701 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     2761 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     2821 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     2881 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2941 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     3001 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     3061 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     3121 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     3181 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     3241 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     3301 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     3361 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     3421 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     3481 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     3541 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     3601 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3661 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     3721 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     3781 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc
     3841 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4561 gtt
//
