LOCUS       sequence-168872-        4869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PRDM1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PRDM1_WT
FEATURES             Location/Qualifiers
     source          1..4869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2748..2847)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2864..2883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2865..2883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2888..2904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2888..2904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3335..4126
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4219..4807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4708..4727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggaaaaga tctattccag aggggagctt caccacttca ttgacggctt
      721 taatgaagag aaaagcaact ggatgcgcta tgtgaatcca gcacactctc cccgggagca
      781 aaacctggct gcgtgtcaga acgggatgaa catctacttc tacaccatta agcccatccc
      841 tgccaaccag gaacttcttg tgtggtattg tcgggacttt gcagaaaggc ttcactaccc
      901 ttatcccgga gagctgacaa tgatgaatct cacacaaaca cagagcagtc taaagcaacc
      961 gagcactgag aaaaatgaac tctgcccaaa gaatgtccca aagagagagt acagcgtgaa
     1021 agaaatccta aaattggact ccaacccctc caaaggaaag gacctctacc gttctaacat
     1081 ttcacccctc acatcagaaa aggacctcga tgactttaga agacgtggga gccccgaaat
     1141 gcccttctac cctcgggtcg tttaccccat ccgggcccct ctgccagaag actttttgaa
     1201 agcttccctg gcctacggga tcgagagacc cacgtacatc actcgctccc ccattccatc
     1261 ctccaccact ccaagcccct ctgcaagaag cagccccgac caaagcctca agagctccag
     1321 ccctcacagc agccctggga atacggtgtc ccctgtgggc cccggctctc aagagcaccg
     1381 ggactcctac gcttacttga acgcgtccta cggcacggaa ggtttgggct cctaccctgg
     1441 ctacgcaccc ctgccccacc tcccgccagc tttcatcccc tcgtacaacg ctcactaccc
     1501 caagttcctc ttgcccccct acggcatgaa ttgtaatggc ctgagcgctg tgagcagcat
     1561 gaatggcatc aacaactttg gcctcttccc gaggctgtgc cctgtctaca gcaatctcct
     1621 cggtgggggc agcctgcccc accccatgct caaccccact tctctcccga gctcgctgcc
     1681 ctcagatgga gcccggaggt tgctccagcc ggagcatccc agggaggtgc ttgtcccggc
     1741 gccccacagt gccttctcct ttaccggggc cgccgccagc atgaaggaca aggcctgtag
     1801 ccccacaagc gggtctccca cggcgggaac agccgccacg gcagaacatg tggtgcagcc
     1861 caaagctacc tcagcagcga tggcagcccc cagcagcgac gaagccatga atctcattaa
     1921 aaacaaaaga aacatgaccg gctacaagac ccttccctac ccgctgaaga agcagaacgg
     1981 caagatcaag tacgaatgca acgtttgcgc caagactttc ggccagctct ccaatctgaa
     2041 ggtccacctg agagtgcaca gtggagaacg gcctttcaaa tgtcagactt gcaacaaggg
     2101 ctttactcag ctcgcccacc tgcagaaaca ctacctggta cacacgggag aaaagccaca
     2161 tgaatgccag gtctgccaca agagatttag cagcaccagc aatctcaaga cccacctgcg
     2221 actccattct ggagagaaac cataccaatg caaggtgtgc cctgccaagt tcacccagtt
     2281 tgtgcacctg aaactgcaca agcgtctgca cacccgggag cggccccaca agtgctccca
     2341 gtgccacaag aactacatcc atctctgtag cctcaaggtt cacctgaaag ggaactgcgc
     2401 tgcggccccg gcgcctgggc tgcccttgga agatctgacc cgaatcaatg aagaaatcga
     2461 gaagtttgac atcagtgaca atgctgaccg gctcgaggac gtggaggatg acatcagtgt
     2521 gatctctgta gtggagaagg aaattctggc cgtggtcaga aaagagaaag aagaaactgg
     2581 cctgaaagtg tctttgcaaa gaaacatggg gaatggactc ctctcctcag ggtgcagcct
     2641 ttatgagtca tcagatctac ccctcatgaa gttgcctccc agcaacccac tacctctggt
     2701 acctgtaaag gtcaaacaag aaacagttga accaatggat ccttgattgc caactttctt
     2761 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2821 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2881 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2941 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     3001 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     3061 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     3121 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     3181 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     3241 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     3301 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     3361 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     3421 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     3481 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     3541 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     3601 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3661 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3721 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3781 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3841 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3901 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3961 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     4021 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     4081 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     4141 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     4201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4321 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4861 tcacatgtt
//
