LOCUS       sequence-168875-        4200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KRT26 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KRT26_WT
FEATURES             Location/Qualifiers
     source          1..4200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2079..2178)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2195..2214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2196..2214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2219..2235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2219..2235)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2666..3457
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3550..4138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4039..4058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtcttttc gactttctgg tggatccagg aggatctgct cgcgaactgg
      721 gtctggtagg ctgtccggtg gaggaacagg cttcgtggct gggaatgtgt gtgttggatc
      781 gggagcaaga agcagctttt cttgtactct tgagggcatc tcttctggag gaagcttctg
      841 caatagcggt ggagggttgg gaagtggtgc ctgtgctggt tttcttggca atgagcacag
      901 cctcctctct gggaatgaaa aggtgaccat gcagaatctc aacgaccgcc tggcatccta
      961 cctggaccat gtgcatgctc tagaggaggc caacgcagac ctggagcaga agatcaaggg
     1021 ctggtacgag aaatgtgagc ctggctcttc ccgggaacac gatcatgact atagcagata
     1081 cttctcagtc atagaagatc ttaaaaggca gattatttct gcgaccatct gcaatgccag
     1141 cattgttcta caaaatgaca atgccagact gaccgctgat gacttcaggc tgaagtatga
     1201 aaatgagctg gctctgcacc acagtgttga ggccgacacc agtggtcttc gcagagtgtt
     1261 ggatgaactg accctttgta caaccgacct ggagatacag tgtgagaccc tcagtgagga
     1321 attgacctac ctcaaaaaaa gtcatgagga ggaaatggaa gtcttgcaat atacagctgg
     1381 ggggaacgtg aacgtggaga tgaatgcaac cccaggagtg gacctaactg tcctgttgaa
     1441 caacatgagg gctgagtatg aggacttggc tgagcagaac cgcaaagatg ctgaagcctg
     1501 gttcaacgag aggagtgcaa cgctgcaaca acagatttcc gatcatgagg gagcagccac
     1561 agcagccaga aatgagctga ccgaattaaa acgcaatctg caaaccctgg aaatagaact
     1621 tcagtccctc atggctgtga aacattccta tgaatgctcc ttggctgaga ctgaaggaaa
     1681 ttactgcaat caactccagc aaattcagga tcagataggg gtgatggagg aacaactgca
     1741 acagattcga acagaaacag aaggccagaa gctggagtat gaacagcttc ttgatgtaaa
     1801 aatattttta gaaaaagaaa ttgacattta ttgcaactta ctagatggag aagaaagaaa
     1861 aagcaaatcc acatgttaca aatcaaaagg atacaggcct gtgaattctg gaaatcaagc
     1921 caaagactca acagaagaaa ctattgttaa aacagtggtt gaggaactgg atcaaattgg
     1981 caatctcctt tcactgagag tccactcagt tgaagaaaag tcctctaaaa taagcaacat
     2041 tacagtagag caacgagtac cttctaaagc accatgattg ccaactttct tgtacaaagt
     2101 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2161 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2221 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2281 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2341 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     2401 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     2461 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2521 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     2581 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     2641 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     2701 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     2761 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     2821 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     2881 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     2941 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3001 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3061 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3121 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3181 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3241 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3301 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3361 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     3421 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     3481 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
