LOCUS       sequence-168882-        4362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ZNF454 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ZNF454_WT
FEATURES             Location/Qualifiers
     source          1..4362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1762..1861)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1878..1897)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1879..1897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1902..1918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1902..1918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2349..3140
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3233..3821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3722..3741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3975..3992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4151..4178)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4270..4356)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggctgtc agccacctgc caaccatggt ccaggaatcg gtgaccttca
      241 aggatgtggc tatactgttc acccaggaag agtgggggca gctgagcccc gcccagaggg
      301 ccctgtacag ggacgtgatg ctggagaact acagcaacct ggtctcactg ggactcttag
      361 gacccaaacc agatacgttt tcccagctag aaaaaaggga agtgtggatg ccagaggaca
      421 cccctggagg cttctgtctt gactggatga ctatgcctgc cagtaagaaa tctactgtca
      481 aggcagagat tcctgaagaa gaattggatc aatggacaat aaaggaaaga ttcagtagca
      541 gtagtcactg gaagtgtgct agcctgctgg agtggcaatg tggaggccag gagatcagtt
      601 tgcagcgagt ggtactcact caccccaaca ccccatcaca ggaatgtgat gaatccggga
      661 gcactatgag ctcatctctt cacagtgctc aaagtcaggg atttcaacct agcaaaaatg
      721 cctttgagtg tagtgagtgt ggaaaagtct tctctaagag ttcaactctt aataaacatc
      781 agaaaattca taatgaaaaa aatgcaaatc agaaaattca tattaaggag aaaagatatg
      841 aatgtagaga atgtgggaaa gcctttcacc agagtacgca ccttatccat caccaaagaa
      901 ttcacactgg cgagaaaccc tatgaatgta aggaatgtgg caaggccttc tcagtgagct
      961 cctcacttac gtaccatcag aaaattcata ctggagagaa gccttttgaa tgcaacttat
     1021 gtggaaaagc ttttatccga aatatacacc ttgcccatca tcatagaata catactggag
     1081 agaaaccttt taaatgtaac atttgtgaaa aagcctttgt gtgcagggca caccttacca
     1141 aacaccagaa tatccacagt ggagagaaac cctataaatg caatgaatgt ggaaaagcct
     1201 ttaatcagag tacaagtttc cttcagcatc agagaattca cactggagag aaaccctttg
     1261 aatgtaatga atgtgggaag gccttcaggg tgaactcttc ccttactgaa catcagagaa
     1321 ttcatactgg agagaaacct tataaatgta atgaatgtgg gaaagctttc agggataatt
     1381 catcctttgc acgacatcgg aaaattcaca ctggagagaa accttacaga tgtggcttgt
     1441 gtgagaaagc ctttcgggac caatcagcac tagcccaaca tcagagaatt catactgggg
     1501 aaaaacctta tacatgtaac atatgtgaaa aagccttcag tgaccattca gcccttaccc
     1561 aacataagag aattcatact agggaaaaac cttacaaatg taaaatctgt gagaaagcct
     1621 ttatccgaag cactcacctg actcaacatc agaggattca cacaggagag aaaccctata
     1681 aatgtaataa atgtgggaaa gcttttaacc agactgcaaa cctcattcag catcagagac
     1741 atcatattgg agagaagtga ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     1801 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1861 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1921 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1981 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     2041 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     2101 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2161 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2221 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2281 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2341 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2401 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2461 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2521 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2581 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2641 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2701 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2761 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2821 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2881 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2941 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3001 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3061 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3121 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     3181 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3361 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4021 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4081 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     4141 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     4201 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     4261 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4321 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
