LOCUS       sequence-168884-        4299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CHD2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CHD2_WT
FEATURES             Location/Qualifiers
     source          1..4299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2178..2277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2294..2313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2295..2313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2318..2334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2318..2334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2765..3556
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3649..4237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4138..4157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgatgagaa ataaggacaa aagccaagag gaggacagtt cgctacacag
      721 caatgcatcg agtcactcag cctctgaaga agcttcgggt tcagactcag gcagtcagtc
      781 ggaaagtgag cagggaagtg atccaggaag tggacatggc agcgagtcga acagcagctc
      841 tgaatcttct gagagtcagt cggaatctga gagcgaatca gcaggttcca aatcccagcc
      901 agtcctccca gaagccaaag agaagccagc ctctaagaag gaacggatag ctgatgtgaa
      961 gaagatgtgg gaagaatatc ctgatgttta tggggtcagg cggtcaaacc gaagcagaca
     1021 agaaccatcg cgatttaata ttaaggaaga ggcaagtagc gggtctgaga gtgggagccc
     1081 aaaaagaaga ggccagaggc agctgaaaaa acaagaaaaa tggaaacagg aaccctcaga
     1141 agatgaacag gaacaaggca ccagtgcaga gagtgagcca gaacaaaaaa aagtaaaagc
     1201 cagaagacct gtccccagaa gaacagtgcc caaacctcgt gttaaaaagc agccgaagac
     1261 tcagcgtgga aagagaaaaa agcaagattc ttctgatgag gatgatgatg atgacgaagc
     1321 tcccaaaagg cagactcgtc gaagagcggc taaaaacgtt agttacaaag aagatgatga
     1381 ctttgagact gactcagatg atctcattga aatgactgga gaaggagttg atgaacagca
     1441 agataatagt gaaactattg aaaaggtctt agattcaaga ctgggaaaga aaggagccac
     1501 tggagcatct actactgtat atgcgattga agctaatggc gaccctagtg gtgactttga
     1561 cactgaaaag gatgaaggtg aaatccagta cctcatcaag tggaagggtt ggtcttacat
     1621 ccacagcaca tgggagagtg aagaatcctt acagcaacag aaagtgaagg gcctaaaaaa
     1681 actagagaac ttcaagaaaa aagaggacga aatcaaacaa tggttaggga aagtttctcc
     1741 tgaagatgta gaatatttca attgccaaca ggagctggct tcagagttga ataaacagta
     1801 tcagatagta gaaagagtaa tagctgtgaa gacaagtaaa tctacattgg gtcaaacaga
     1861 ttttccagct catagtcgga agccggcacc ctcaaatgag cccgaatatc tatgtaaatg
     1921 gatgggactc ccctattcag agtgtagctg ggaagatgaa gccctcattg gaaagaaatt
     1981 ccagaattgc attgacagct tccacagtag gaacaactca aaaaccatcc caacaagaga
     2041 atgcaaggcc ctgaagcaga gaccacgatt tgtagcttta aagaaacaac ctgcatattt
     2101 aggaggggag aatctggaac ttcgagatta tcagctagaa ggtctaaact ggctagctca
     2161 ttcctggtgc aagtgattgc caactttctt gtacaaagtt ggcattataa gaaagcattg
     2221 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2281 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2341 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2401 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2461 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2521 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2581 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2641 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2701 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     2761 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     2821 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     2881 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     2941 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3001 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3061 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3121 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3181 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3241 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3301 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3361 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3421 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3481 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3541 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3601 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4261 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
