LOCUS       sequence-168887-        4611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NFE2L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NFE2L2_WT
FEATURES             Location/Qualifiers
     source          1..4611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2490..2589)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2606..2625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2607..2625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2630..2646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2630..2646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3077..3868
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3961..4549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4450..4469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgatggact tggagctgcc gccgccggga ctcccgtccc agcaggacat
      721 ggatttgatt gacatacttt ggaggcaaga tatagatctt ggagtaagtc gagaagtatt
      781 tgacttcagt cagcgacgga aagagtatga gctggaaaaa cagaaaaaac ttgaaaagga
      841 aagacaagaa caactccaaa aggagcaaga gaaagccttt ttcgctcagt tacaactaga
      901 tgaagagaca ggtgaatttc tcccaattca gccagcccag cacatccagt cagaaaccag
      961 tggatctgcc aactactccc aggttgccca cattcccaaa tcagatgctt tgtactttga
     1021 tgactgcatg cagcttttgg cgcagacatt cccgtttgta gatgacaatg aggtttcttc
     1081 ggctacgttt cagtcacttg ttcctgatat tcccggtcac atcgagagcc cagtcttcat
     1141 tgctactaat caggctcagt cacctgaaac ttctgttgct caggtagccc ctgttgattt
     1201 agacggtatg caacaggaca ttgagcaagt ttgggaggag ctattatcca ttcctgagtt
     1261 acagtgtctt aatattgaaa atgacaagct ggttgagact accatggttc caagtccaga
     1321 agccaaactg acagaagttg acaattatca tttttactca tctataccct caatggaaaa
     1381 agaagtaggt aactgtagtc cacattttct taatgctttt gaggattcct tcagcagcat
     1441 cctctccaca gaagacccca accagttgac agtgaactca ttaaattcag atgccacagt
     1501 caacacagat tttggtgatg aattttattc tgctttcata gctgagccca gtatcagcaa
     1561 cagcatgccc tcacctgcta ctttaagcca ttcactctct gaacttctaa atgggcccat
     1621 tgatgtttct gatctatcac tttgcaaagc tttcaaccaa aaccaccctg aaagcacagc
     1681 agaattcaat gattctgact ccggcatttc actaaacaca agtcccagtg tggcatcacc
     1741 agaacactca gtggaatctt ccagctatgg agacacacta cttggcctca gtgattctga
     1801 agtggaagag ctagatagtg cccctggaag tgtcaaacag aatggtccta aaacaccagt
     1861 acattcttct ggggatatgg tacaaccctt gtcaccatct caggggcaga gcactcacgt
     1921 gcatgatgcc caatgtgaga acacaccaga gaaagaattg cctgtaagtc ctggtcatcg
     1981 gaaaacccca ttcacaaaag acaaacattc aagccgcttg gaggctcatc tcacaagaga
     2041 tgaacttagg gcaaaagctc tccatatccc attccctgta gaaaaaatca ttaacctccc
     2101 tgttgttgac ttcaacgaaa tgatgtccaa agagcagttc aatgaagctc aacttgcatt
     2161 aattcgggat atacgtagga ggggtaagaa taaagtggct gctcagaatt gcagaaaaag
     2221 aaaactggaa aatatagtag aactagagca agatttagat catttgaaag atgaaaaaga
     2281 aaaattgctc aaagaaaaag gagaaaatga caaaagcctt cacctactga aaaaacaact
     2341 cagcacctta tatctcgaag ttttcagcat gctacgtgat gaagatggaa aaccttattc
     2401 tcctagtgaa tactccctgc agcaaacaag agatggcaat gttttccttg ttcccaaaag
     2461 taagaagcca gatgttaaga aaaactgatt gccaactttc ttgtacaaag ttggcattat
     2521 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2581 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2641 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2701 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     2761 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2821 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2881 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2941 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     3001 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3061 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3121 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     3181 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     3241 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3301 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     3361 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     3421 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3481 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3541 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3601 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3661 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3721 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3781 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3841 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3901 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
