LOCUS       sequence-168897-        4395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TPTE , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TPTE_WT
FEATURES             Location/Qualifiers
     source          1..4395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1795..1894)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1911..1930)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1912..1930)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1935..1951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1935..1951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2382..3173
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3266..3854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3755..3774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4008..4025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4184..4211)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4303..4389)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgaatgaa agtcctgatc cgactgacct ggcgggagtc atcattgagc
      241 tcggccccaa tgacagtcca cagacaagtg aatttaaagg agcaaccgag gaggcacctg
      301 cgaaagaaag tgtgttagca cgactttcca agtttgaagt tgaagatgct gaaaatgttg
      361 cttcatatga cagcaagatt aagaaaattg tgcattcaat tgtatcatcc tttgcatttg
      421 gactatttgg agttttcctg gtcttactgg atgtcactct catccttgcc gacctaattt
      481 tcactgacag caaactttat attcctttgg agtatcgttc tatttctcta gctattgcct
      541 tattttttct catggatgtt cttcttcgag tatttgtaga aaggagacag cagtattttt
      601 ctgacttatt taacatttta gatactgcca ttattgtgat tcttctgctg gttgatgtcg
      661 tttacatttt ttttgacatt aagttgctta ggaatattcc cagatggaca catttacttc
      721 gacttctacg acttattatt ctgttaagaa tttttcatct gtttcatcaa aaaagacaac
      781 ttgaaaagct gataagaagg cgggtttcag aaaacaaaag gcgatacaca agggatggat
      841 ttgacctaga cctcacttac gttacagaac gtattattgc tatgtcattt ccatcttctg
      901 gaaggcagtc tttctataga aatccaatca aggaagttgt gcggtttcta gataagaaac
      961 accgaaacca ctatcgagtc tacaatctat gcagtgaaag agcttacgat cctaagcact
     1021 tccataatag ggtcgttaga atcatgattg atgatcataa tgtccccact ctacatcaga
     1081 tggtggtttt caccaaggaa gtaaatgagt ggatggctca agatcttgaa aacatcgtag
     1141 cgattcactg taaaggaggc acagatagaa caggaactat ggtttgtgcc ttccttattg
     1201 cctctgaaat atgttcaact gcaaaggaaa gcctgtatta ttttggagaa aggcgaacag
     1261 ataaaaccca cagcgaaaaa tttcagggag tagaaactcc ttctcagaag agatatgttg
     1321 catattttgc acaagtgaaa catctctaca actggaatct ccctccaaga cggatactct
     1381 ttataaaaca cttcattatt tattcgattc ctcgttatgt acgtgatcta aaaatccaaa
     1441 tagaaatgga gaaaaaggtt gtcttttcca ctatttcatt aggaaaatgt tcggtacttg
     1501 ataacattac aacagacaaa atattaattg atgtattcga cggtccacct ctgtatgatg
     1561 atgtgaaagt gcagtttttc tattcgaatc ttcctacata ctatgacaat tgctcatttt
     1621 acttctggtt gcacacatct tttattgaaa ataacaggct ttatctacca aaaaatgaat
     1681 tggataatct acataaacaa aaagcacgga gaatttatcc atcagatttt gccgtggaga
     1741 tactttttgg cgagaaaatg acttccagtg atgttgtagc tggatccgat tgattgccaa
     1801 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1861 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1921 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1981 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     2041 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2101 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2161 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2221 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2281 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2341 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2401 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2461 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2521 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2581 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2641 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2701 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2761 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2821 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2881 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2941 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3001 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3061 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3121 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3181 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3841 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3901 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3961 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4021 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4081 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4141 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4201 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4261 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4321 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4381 ttttatttga tgcct
//
