LOCUS       sequence-168902-        4914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BCL6 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BCL6_WT
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2314..2413)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2430..2449)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2431..2449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2454..2470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2454..2470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2901..3692
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3785..4373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4274..4293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4527..4544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4703..4730)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4822..4908)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttatacaaaa
      181 aagttggcac catggcctcg ccggctgaca gctgtatcca gttcacccgc catgccagtg
      241 atgttcttct caaccttaat cgtctccgga gtcgagacat cttgactgat gttgtcattg
      301 ttgtgagccg tgagcagttt agagcccata aaacggtcct catggcctgc agtggcctgt
      361 tctatagcat ctttacagac cagttgaaat gcaaccttag tgtgatcaat ctagatcctg
      421 agatcaaccc tgagggattc tgcatcctcc tggacttcat gtacacatct cggctcaatt
      481 tgcgggaggg caacatcatg gctgtgatgg ccacggctat gtacctgcag atggagcatg
      541 ttgtggacac ttgccggaag tttattaagg ccagtgaagc agagatggtt tctgccatca
      601 agcctcctcg tgaagagttc ctcaacagcc gtatgctgat gccccaagac atcatggcct
      661 atcggggttg tgaggtggtg gagaacaacc tgccactgag gagcgcccct gggtgtgaga
      721 gcagagcctt tgcccccagc ctgtacagtg gcctgtccac accgccagcc tcttattcca
      781 tgtacagcca cctccctgtc agcagcctcc tcttctccga tgaggagttt cgggatgtcc
      841 ggatgcctgt ggccaacccc ttccccaagg agcgggcact cccatgtgat agtgccaggc
      901 cagtccctgg tgagtacagc cggccgactt tggaggtgtc ccccaatgtg tgccacagca
      961 atatctattc acccaaggaa acaatcccag aagaggcacg aagtgatatg cactacagtg
     1021 tggctgaggg cctcaaacct gctgccccct cagcccgaaa tgccccctac ttcccttgtg
     1081 acaaggccag caaagaagaa gagagaccct cctcggaaga tgagattgcc ctgcatttcg
     1141 agccccccaa tgcacccctg aaccggaagg gtctggttag tccacagagc ccccagaaat
     1201 ctgactgcca gcccaactcg cccacagagt cctgcagcag taagaatgcc tgcatcctcc
     1261 aggcttctgg ctcccctcca gccaagagcc ccactgaccc caaagcctgc aactggaaga
     1321 aatacaagtt catcgtgctc aacagcctca atcagaatgc caaaccagag gggcctgagc
     1381 aggctgagct gggccgcctt tccccacgag cctacacggc cccacctgcc tgccagccac
     1441 ccatggagcc tgagaacctt gacctccagt ccccaaccaa gctgagtgcc agcggggagg
     1501 actccaccat cccacaagcc agccggctca ataacatcgt taacaggtcc atgacgggct
     1561 ctccccgcag cagcagcgag agccactcac cactctacat gcaccccccg aagtgcacgt
     1621 cctgcggctc tcagtcccca cagcatgcag agatgtgcct ccacaccgct ggccccacgt
     1681 tccctgagga gatgggagag acccagtctg agtactcaga ttctagctgt gagaacgggg
     1741 ccttcttctg caatgagtgt gactgccgct tctctgagga ggcctcactc aagaggcaca
     1801 cgctgcagac ccacagtgac aaaccctaca agtgtgaccg ctgccaggcc tccttccgct
     1861 acaagggcaa cctcgccagc cacaagaccg tccataccgg tgagaaaccc tatcgttgca
     1921 acatctgtgg ggcccagttc aaccggccag ccaacctgaa aacccacact cgaattcact
     1981 ctggagagaa gccctacaaa tgcgaaacct gcggagccag atttgtacag gtggcccacc
     2041 tccgtgccca tgtgcttatc cacactggtg agaagcccta tccctgtgaa atctgtggca
     2101 cccgtttccg gcaccttcag actctgaaga gccacctgcg aatccacaca ggagagaaac
     2161 cttaccattg tgagaagtgt aacctgcatt tccgtcacaa aagccagctg cgacttcact
     2221 tgcgccagaa gcatggcgcc atcaccaaca ccaaggtgca ataccgcgtg tcagccactg
     2281 acctgcctcc ggagctcccc aaagcctgct gattgccaac tttcttgtac aaagttggca
     2341 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2401 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2461 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2521 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2581 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2641 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2701 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2761 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2821 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2881 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2941 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3001 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3061 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3121 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3181 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3241 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3301 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3361 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3421 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3481 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3541 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3601 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3661 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3721 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3781 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3841 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3901 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3961 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4021 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4081 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4141 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4201 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4261 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4321 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4381 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4441 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4501 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4561 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4621 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4681 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4741 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4801 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4861 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct
//
