LOCUS       sequence-168906-        4800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SCEL , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SCEL_WT
FEATURES             Location/Qualifiers
     source          1..4800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2200..2299)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2316..2335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2317..2335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2340..2356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2340..2356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2787..3578
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3671..4259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4160..4179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4413..4430
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4589..4616)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4708..4794)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtccaat gttaccttga gaaaaatgtc tcccacagga aatgagatga
      241 agagcaccac tcagggaacc acacggaagc agcaggattt tcacgaggtg aacaaaagaa
      301 gaactttctt acaggataac agttggataa agaaacgccc tgaagaagaa aaagatgaaa
      361 attacggtag ggtggtgctc aaccgacata attcccatga tgcattggac aggaaagtaa
      421 atgagagaga tgtgccaaaa gctacaatta gtcggtacag ttctgatgac actttggaca
      481 ggatctcaga cagaaatgat gctgctaaaa catataaggc caataccttg gataaccaac
      541 taaccaatag gagcatgtcc gtgtttagat cactggaagt aacaaagttg caacctggcg
      601 gttcattgaa tgccaacacc tccaacacca tagcatccac ttctgctact actcctgtaa
      661 agaagaagag gcagtcctgg tttccaccgc cccctccagg ttacaatgcc tcttcgagca
      721 caggaaccag gagacgggaa ccaggtgttc accctccaat acctccaaag cccagttctc
      781 ctgtttcttc tactaaccag ctgagacagg ataataggca gatacatcca cctaaaccag
      841 gtgtatatac agaaaccaac agatctgctg aaagaaatat aagtgaagaa ttggataatc
      901 tcatcaaaat gaacaaaagc ttgaatagga atcaaggtct tgatagtctc ttcagagcaa
      961 atccaaaggt agaagaaaga gagaaaagag ccaaaagcct tgaaagtctc atctatatga
     1021 gtacccggac agataaagat ggcaaaggaa tccaaagcct tggaagtccg attaaagtta
     1081 atcaaaggac tgacaaaaat gagaaaggaa gacaaaatct cgaatctgtt gctaaagtga
     1141 atgccaggat gaataaaacg agcagaagaa gtgaagacct tgataatgct actgaagtaa
     1201 atcccaaagg acatgaaaat accactggaa aaaaagacct tgatgggctt attaaagtgg
     1261 atcctgaaac aaataaaaat attacgaggg gccagagcct tgataatctc atcaaagtga
     1321 cccctgaagt aaagagaagt aaccaaggtt ccaaagacct taataacttc atcaaagtgt
     1381 atccaggaac agaaaaaagt actgaagggg gccaaagtct cgacagcctc attaaagtga
     1441 ctcctgaaag aaacagaact aaccaaggga accaagactt ggaaaatctt atcaaagtga
     1501 tcccttcagc aaacaaaagc agtgaacaag gtcttgatga acatattaat gtcagcccca
     1561 aagctgtcaa aaacactgat ggaaaacaag atcttgataa actcatcaag gtgaatcctg
     1621 aaattttcac aaacaaccaa agaaaccaag atcttgctaa cctcatcaaa gtaaatcctg
     1681 cagtaatcag aaacaatcag agccaagact tggacaatct tattaaagtg aaaccttcag
     1741 ctcttagaaa cactaatcga gaccagaacc tggaaaattt aattgaagta aattctcatg
     1801 tgtctgaaaa caagaatgga agctctaaca ctggagcaaa gcaggcagga ccacaggata
     1861 ctgttgtgta cacaaggaca tatgtggaga atagtaaatc acccaaggat ggatatcagg
     1921 agaatatctc tggaaaatac atacaaactg tttattcaac ttctgatagg tctgtcattg
     1981 aaagagatat gtgcacttac tgccgaaaac ccttgggtgt agaaactaaa atgattttag
     2041 atgaattaca aatttgctgc cattctactt gctttaagtg tgaaatatgc aagcagcctt
     2101 tggaaaatct acaagcgggt gatagtattt ggatttatag acagacaata cactgtgaac
     2161 cttgctactc taaaattatg gcaaagtgga ttccatgatt gccaactttc ttgtacaaag
     2221 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2281 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2341 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2401 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     2461 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2521 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2581 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2641 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2701 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2761 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2821 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2881 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2941 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     3001 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     3061 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     3121 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3181 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3241 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3301 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3361 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3421 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3481 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3541 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3601 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3661 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3721 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3781 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3841 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3901 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3961 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4021 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4081 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4141 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4201 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4261 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4321 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4381 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4441 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4501 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4561 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4621 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4681 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4741 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
//
