LOCUS       sequence-168927-       14121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For ubiquitous expression of Cas9 in planta. sgRNA can be introduced
            as described in Hahn et al. (2017).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP
  TITLE     An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis 
            thaliana.
  JOURNAL   Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. 
            eCollection 2017.
  PUBMED    28174584
REFERENCE   2  (bases 1 to 14121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-01-24"; volume: "8:39. doi"; pages: " 
            10.3389/fpls.2017.00039. eCollection 2017"
COMMENT     Sequence Label: pUB-Cas9
FEATURES             Location/Qualifiers
     source          1..14121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..29)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..29)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(26..48)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(61..67,68..85,86..91))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        318..995
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1063..2088
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2128..2302
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2380..2404
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2829..3623
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3710..4298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4199..4218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4452..4469
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4484..4624
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4550..4569)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4710..4732
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4968..5162
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5228..6295)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6729..7358)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8658..8682
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8870..8892
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8884..8901
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8885..8901
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9610..9634
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9655..9675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9676..13779
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9676..13776
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(13781..13805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
       61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt
      241 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
      301 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
      361 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
      421 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
      481 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
      541 acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctcgtctac
      601 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      661 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      721 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      781 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      841 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
      901 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagacct tcctctatat
      961 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
     1021 tatctctctc gagctttcgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc
     1081 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     1141 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     1201 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     1261 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt tagcgagagc
     1321 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     1381 gaactgcccg ctgttctaca accggtcgcg gaggctatgg atgcgatcgc tgcggccgat
     1441 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     1501 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     1561 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     1621 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     1681 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     1741 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     1801 cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg ggcgtatatg
     1861 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     1921 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     1981 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     2041 gatagtggaa accgacgccc cagcactcgt ccgagggcaa agaaatagag tagatgccga
     2101 ccggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
     2161 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     2221 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     2281 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
     2341 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     2401 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     2461 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     2521 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     2581 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     2641 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
     2701 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
     2761 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
     2821 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
     2881 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
     2941 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
     3001 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
     3061 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
     3121 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
     3181 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
     3241 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
     3301 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
     3361 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
     3421 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
     3481 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
     3541 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
     3601 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
     3661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3841 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4321 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4381 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4441 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     4501 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     4561 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     4621 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4681 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4741 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     4801 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     4861 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     4921 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     4981 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     5041 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     5101 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     5161 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     5221 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     5281 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     5341 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     5401 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     5461 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     5521 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     5581 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     5641 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     5701 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     5761 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     5821 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     5881 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     5941 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     6001 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     6061 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     6121 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     6181 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     6241 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgtcac
     6301 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
     6361 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
     6421 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
     6481 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
     6541 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
     6601 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
     6661 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
     6721 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
     6781 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
     6841 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
     6901 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
     6961 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
     7021 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
     7081 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
     7141 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
     7201 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
     7261 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
     7321 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
     7381 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
     7441 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
     7501 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
     7561 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
     7621 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
     7681 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
     7741 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
     7801 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
     7861 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
     7921 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
     7981 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
     8041 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
     8101 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
     8161 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
     8221 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
     8281 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
     8341 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
     8401 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
     8461 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
     8521 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
     8581 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgtt attctaataa
     8641 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
     8701 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc gccattcagg
     8761 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8821 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8881 cgttgtaaaa cgacggccag tgccaagctt gattacgaat tcgagctcgg tacccgacga
     8941 gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac
     9001 tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg
     9061 cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta
     9121 gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc
     9181 aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc
     9241 tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc
     9301 gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc
     9361 ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg
     9421 gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt
     9481 cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgta
     9541 gattaatctg agtttttctg attaacagct cgagtgcggg ccccccctcg aggcgcgcca
     9601 agctatcaaa caagtttgta caaaaaagca ggctaccata tggccagcag cccccccaag
     9661 aagaagcgca aggtcgacaa gaagtactcc atcggcctgg acatcggcac gaacagcgtg
     9721 ggctgggccg tgatcaccga cgagtacaag gtgccgtcca agaagttcaa ggtgctgggc
     9781 aacaccgacc gccacagcat caagaagaac ctgatcggcg ccctgctgtt cgactcgggc
     9841 gagaccgccg aggcgacgcg cctgaagcgc accgcgcgcc gccgctacac gcgccgcaag
     9901 aaccgcatct gctacctgca ggagatcttc agcaacgaga tggccaaggt ggacgactcg
     9961 ttcttccacc gcctggagga gtccttcctg gtggaggagg acaagaagca cgagcgccac
    10021 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc gacgatctac
    10081 cacctgcgca agaagctggt ggacagcacc gacaaggcgg acctgcgcct gatctacctg
    10141 gccctggcgc acatgatcaa gttccgcggc cacttcctga tcgagggcga cctgaacccc
    10201 gacaactcgg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
    10261 gaggagaacc cgatcaacgc ctcgggcgtg gacgccaagg cgatcctgtc cgcgcgcctg
    10321 tccaagagcc gccgcctgga gaacctgatc gcccagctgc ccggcgagaa gaagaacggc
    10381 ctgttcggca acctgatcgc gctgagcctg ggcctgacgc cgaacttcaa gtcgaacttc
    10441 gacctggccg aggacgcgaa gctgcagctg agcaaggaca cctacgacga cgacctggac
    10501 aacctgctgg cccagatcgg cgaccagtac gcggacctgt tcctggccgc gaagaacctg
    10561 tcggacgcca tcctgctgtc cgacatcctg cgcgtgaaca ccgagatcac gaaggccccc
    10621 ctgtcggcgt ccatgatcaa gcgctacgac gagcaccacc aggacctgac cctgctgaag
    10681 gcgctggtgc gccagcagct gccggagaag tacaaggaga tcttcttcga ccagtccaag
    10741 aacggctacg ccggctacat cgacggcggc gccagccagg aggagttcta caagttcatc
    10801 aagcccatcc tggagaagat ggacggcacg gaggagctgc tggtgaagct gaaccgcgag
    10861 gacctgctgc gcaagcagcg caccttcgac aacggcagca tcccccacca gatccacctg
    10921 ggcgagctgc acgccatcct gcgccgccag gaggacttct acccgttcct gaaggacaac
    10981 cgcgagaaga tcgagaagat cctgacgttc cgcatcccct actacgtggg cccgctggct
    11041 cgcggcaact cccgcttcgc gtggatgacc cgcaagagcg aggagaccat cacgccctgg
    11101 aacttcgagg aggtggtgga caagggcgcc agcgcgcagt cgttcatcga gcgcatgacc
    11161 aacttcgaca agaacctgcc caacgagaag gtgctgccga agcactccct gctgtacgag
    11221 tacttcaccg tgtacaacga gctgacgaag gtgaagtacg tgaccgaggg catgcgcaag
    11281 cccgccttcc tgagcggcga gcagaagaag gcgatcgtgg acctgctgtt caagaccaac
    11341 cgcaaggtga cggtgaagca gctgaaggag gactacttca agaagatcga gtgcttcgac
    11401 agcgtggaga tctcgggcgt ggaggaccgc ttcaacgcca gcctgggcac ctaccacgac
    11461 ctgctgaaga tcatcaagga caaggacttc ctggacaacg aggagaacga ggacatcctg
    11521 gaggacatcg tgctgaccct gacgctgttc gaggaccgcg agatgatcga ggagcgcctg
    11581 aagacgtacg cccacctgtt cgacgacaag gtgatgaagc agctgaagcg ccgccgctac
    11641 accggctggg gccgcctgag ccgcaagctg atcaacggca tccgcgacaa gcagtcgggc
    11701 aagaccatcc tggacttcct gaagtccgac ggcttcgcga accgcaactt catgcagctg
    11761 atccacgacg actcgctgac cttcaaggag gacatccaga aggcccaggt gtcgggccag
    11821 ggcgactccc tgcacgagca catcgccaac ctggcgggct cccccgcgat caagaagggc
    11881 atcctgcaga ccgtgaaggt ggtggacgag ctggtgaagg tgatgggccg ccacaagccg
    11941 gagaacatcg tgatcgagat ggcccgcgag aaccagacca cgcagaaggg ccagaagaac
    12001 agccgcgagc gcatgaagcg catcgaggag ggcatcaagg agctgggctc gcagatcctg
    12061 aaggagcacc ccgtggagaa cacccagctg cagaacgaga agctgtacct gtactacctg
    12121 cagaacggcc gcgacatgta cgtggaccag gagctggaca tcaaccgcct gtccgactac
    12181 gacgtggacc acatcgtgcc ccagagcttc ctgaaggacg actcgatcga caacaaggtg
    12241 ctgacccgca gcgacaagaa ccgcggcaag agcgacaacg tgccgtcgga ggaggtggtg
    12301 aagaagatga agaactactg gcgccagctg ctgaacgcca agctgatcac gcagcgcaag
    12361 ttcgacaacc tgaccaaggc cgagcgcggc ggcctgtcgg agctggacaa ggcgggcttc
    12421 atcaagcgcc agctggtgga gacccgccag atcacgaagc acgtggcgca gatcctggac
    12481 tcccgcatga acacgaagta cgacgagaac gacaagctga tccgcgaggt gaaggtgatc
    12541 accctgaagt ccaagctggt cagcgacttc cgcaaggact tccagttcta caaggtgcgc
    12601 gagatcaaca actaccacca cgcccacgac gcgtacctga acgccgtggt gggcaccgcg
    12661 ctgatcaaga agtaccccaa gctggagagc gagttcgtgt acggcgacta caaggtgtac
    12721 gacgtgcgca agatgatcgc caagtcggag caggagatcg gcaaggccac cgcgaagtac
    12781 ttcttctact ccaacatcat gaacttcttc aagaccgaga tcacgctggc caacggcgag
    12841 atccgcaagc gccccctgat cgagaccaac ggcgagacgg gcgagatcgt gtgggacaag
    12901 ggccgcgact tcgcgaccgt gcgcaaggtg ctgagcatgc cccaggtgaa catcgtgaag
    12961 aagaccgagg tgcagacggg cggcttctcc aaggagagca tcctgccgaa gcgcaactcg
    13021 gacaagctga tcgcccgcaa gaaggactgg gaccccaaga agtacggcgg cttcgactcc
    13081 ccgaccgtgg cctacagcgt gctggtggtg gcgaaggtgg agaagggcaa gtccaagaag
    13141 ctgaagagcg tgaaggagct gctgggcatc accatcatgg agcgcagctc gttcgagaag
    13201 aaccccatcg acttcctgga ggcgaagggc tacaaggagg tgaagaagga cctgatcatc
    13261 aagctgccga agtactcgct gttcgagctg gagaacggcc gcaagcgcat gctggcctcc
    13321 gcgggcgagc tgcagaaggg caacgagctg gccctgccca gcaagtacgt gaacttcctg
    13381 tacctggcgt cccactacga gaagctgaag ggctcgccgg aggacaacga gcagaagcag
    13441 ctgttcgtgg agcagcacaa gcactacctg gacgagatca tcgagcagat ctcggagttc
    13501 tccaagcgcg tgatcctggc cgacgcgaac ctggacaagg tgctgagcgc ctacaacaag
    13561 caccgcgaca agcccatccg cgagcaggcg gagaacatca tccacctgtt caccctgacg
    13621 aacctgggcg ccccggccgc gttcaagtac ttcgacacca cgatcgaccg caagcgctac
    13681 accagcacga aggaggtgct ggacgcgacc ctgatccacc agagcatcac cggcctgtac
    13741 gagacgcgca tcgacctgtc gcagctgggc ggcgactaat acccagcttt cttgtacaaa
    13801 gtggttcgat aattccttaa ttaactagtt ctagagcggc cgcccaccgc ggtggagctc
    13861 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
    13921 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
    13981 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
    14041 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
    14101 ggtgtcatct atgttactga a
//
