LOCUS       sequence-168932-       10415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pheromone mediated expression of UBiC, ARO4, and TKL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Williams TC, Averesch NJH, Winter G, Plan MR, Vickers CE, Nielsen 
            LK, Kromer JO
  TITLE     Quorum-sensing linked RNA interference for dynamic metabolic pathway
            control in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2015 May;29:124-134. doi: 10.1016/j.ymben.2015.03.008. 
            Epub 2015 Mar 16.
  PUBMED    25792511
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-05"; volume: "29:124-134. doi"; pages: " 
            10.1016/j.ymben.2015.03.008. Epub 2015 Mar 16"
COMMENT     Sequence Label: pTCW022
FEATURES             Location/Qualifiers
     source          1..10415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1488..1509)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1700..1719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1936..1958
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1950..1967
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1951..1967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2988..3004)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3028..3277
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4940..4956)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4980..5229
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     terminator      7889..8136
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8194..8210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8194..8210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8207..8229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8218..8234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8242..8248,8249..8266,8267..8272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8425..8442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8596..9184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8676..8695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9355..10146,10147..10215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9978..9997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10216..10320)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10388..10406
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcccctc
     1981 cttcaatttt tctggcaact tttctcaaca gaacaataac ggcaaccaga accgctactg
     2041 aacgatgatt cagttcgcct tctatccttt gttttcgtat ttgtttatat atataacttt
     2101 attttttttt attaattggg ctgcaagaca attttgttgt cagtgatgcc tcaatccttc
     2161 ttttgcttcc atatttacca tgtggaccct ttcaaaacag agttgtatct ctgcaggatg
     2221 ccctttttga cgtattgaat ggcataattg cactgtcact tttcgagctg tcccattttg
     2281 gtgcgatgat ggaacaaaca tgaaacgtct gtaatttgaa acaaataacg taattctcgg
     2341 gattggtttt atttaaatga caatgtaaga gtggctttgt aaggtatgtg ttgctcttaa
     2401 aatatttgga tacgacttcc gttatctttt ttcctttaag agcaggatat aagccaacaa
     2461 gtttctgaaa atcaaagaat tcatgtccca tccagctttg acacaattga gagctttgag
     2521 atactgcaaa gaaattccag cattggatcc acaattattg gactggttgt tgttggaaga
     2581 ttccatgact aagagattcg aacaacaagg taagaccgtt tccgttacca tgattagaga
     2641 aggtttcgtc gaacaaaacg aaatcccaga agaattgcct ttgttgccaa aagaatctag
     2701 atactggttg agagaaattt tgttgtgcgc tgatggtgaa ccttggttgg ctggtagaac
     2761 tgttgttcca gtttctactt tgtctggtcc agaattggca ttgcaaaaat tgggtaaaac
     2821 tccattgggt agatacttgt tcacttcttc taccttgacc agagacttca ttgaaattgg
     2881 tagagatgct ggtttgtggg gtagaagatc aagattgaga ttgtctggta aacctttgtt
     2941 gttgaccgaa ttattcttgc cagcttcccc attatactga cccgggggat ccactagttc
     3001 tagaaggccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
     3061 gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     3121 tccctattta ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat
     3181 ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct
     3241 tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag cttcgcagtt tacactctca
     3301 tcgtcgctcg agccctcctt caatttttct ggcaactttt ctcaacagaa caataacggc
     3361 aaccagaacc gctactgaac gatgattcag ttcgccttct atcctttgtt ttcgtatttg
     3421 tttatatata taactttatt tttttttatt aattgggctg caagacaatt ttgttgtcag
     3481 tgatgcctca atccttcttt tgcttccata tttaccatgt ggaccctttc aaaacagagt
     3541 tgtatctctg caggatgccc tttttgacgt attgaatggc ataattgcac tgtcactttt
     3601 cgagctgtcc cattttggtg cgatgatgga acaaacatga aacgtctgta atttgaaaca
     3661 aataacgtaa ttctcgggat tggttttatt taaatgacaa tgtaagagtg gctttgtaag
     3721 gtatgtgttg ctcttaaaat atttggatac gacttccgtt atcttttttc ctttaagagc
     3781 aggatataag ccaacaagtt tctgaaaatc aaagaattca tgagtgaatc tccaatgttc
     3841 gctgccaacg gcatgccaaa ggtaaatcaa ggtgctgaag aagatgtcag aattttaggt
     3901 tacgacccat tagcttctcc agctctcctt caagtgcaaa tcccagccac accaacttct
     3961 ttggaaactg ccaagagagg tagaagagaa gctatagata ttattaccgg taaagacgac
     4021 agagttcttg tcattgtcgg tccttgttcc atccatgatc tagaagccgc tcaagaatac
     4081 gctttgagat taaagaaatt gtcagatgaa ttaaaaggtg atttatccat cattatgaga
     4141 gcatacttgg agaagccaag aacaaccgtc ggctggaaag gtctaattaa tgaccctgat
     4201 gttaacaaca ctttcaacat caacaagggt ttgcaatccg ctagacaatt gtttgtcaac
     4261 ttgacaaata tcggtttgcc aattggttct gaaatgcttg ataccatttc tcctaaatac
     4321 ttggctgatt tggtctcctt cggtgccatt ggtgccagaa ccaccgaatc tcaactgcac
     4381 agagaattgg cctccggttt gtctttccca gttggtttca agaacggtac cgatggtacc
     4441 ttaaatgttg ctgtggatgc ttgtcaagcc gctgctcatt ctcaccattt catgggtgtt
     4501 actaagcatg gtgttgctgc tatcaccact actaagggta acgaacactg cttcgttatt
     4561 ctaagaggtg gtaaaaaggg taccaactac gacgctaagt ccgttgcaga agctaaggct
     4621 caattgcctg ccggttccaa cggtctaatg attgactact ctcacggtaa ctccaataag
     4681 gatttcagaa accaaccaaa ggtcaatgac gttgtttgtg agcaaatcgc taacggtgaa
     4741 aacgccatta ccggtgtcat gattgaatca aacatcaacg aaggtaacca aggcatccca
     4801 gccgaaggta aagccggctt gaaatatggt gtttccatca ctgatgcttg tataggttgg
     4861 gaaactactg aagacgtctt gaggaaattg gctgctgctg tcagacaaag aagagaagtt
     4921 aacaagaaat agcccggggg atccactagt tctagaaggc cccttttcct ttgtcgatat
     4981 catgtaatta gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga
     5041 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tatttttttt aatagttatg
     5101 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta caaacgcgtg
     5161 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     5221 ttaatttgca agcttcgcag tttacactct catcgtcgag ctccagcttt tgttcccttt
     5281 cctaggtccg gatattatgc ggccgcgccc tccttcaatt tttctggcaa cttttctcaa
     5341 cagaacaata acggcaacca gaaccgctac tgaacgatga ttcagttcgc cttctatcct
     5401 ttgttttcgt atttgtttat atatataact ttattttttt ttattaattg ggctgcaaga
     5461 caattttgtt gtcagtgatg cctcaatcct tcttttgctt ccatatttac catgtggacc
     5521 ctttcaaaac agagttgtat ctctgcagga tgcccttttt gacgtattga atggcataat
     5581 tgcactgtca cttttcgagc tgtcccattt tggtgcgatg atggaacaaa catgaaacgt
     5641 ctgtaatttg aaacaaataa cgtaattctc gggattggtt ttatttaaat gacaatgtaa
     5701 gagtggcttt gtaaggtatg tgttgctctt aaaatatttg gatacgactt ccgttatctt
     5761 ttttccttta agagcaggat ataagccaac aagtttctga aaatcaaaga attcatgact
     5821 caattcactg acattgataa gctagccgtc tccaccataa gaattttggc tgtggacacc
     5881 gtatccaagg ccaactcagg tcacccaggt gctccattgg gtatggcacc agctgcacac
     5941 gttctatgga gtcaaatgcg catgaaccca accaacccag actggatcaa cagagataga
     6001 tttgtcttgt ctaacggtca cgcggtcgct ttgttgtatt ctatgctaca tttgactggt
     6061 tacgatctgt ctattgaaga cttgaaacag ttcagacagt tgggttccag aacaccaggt
     6121 catcctgaat ttgagttgcc aggtgttgaa gttactaccg gtccattagg tcaaggtatc
     6181 tccaacgctg ttggtatggc catggctcaa gctaacctgg ctgccactta caacaagccg
     6241 ggctttacct tgtctgacaa ctacacctat gttttcttgg gtgacggttg tttgcaagaa
     6301 ggtatttctt cagaagcttc ctccttggct ggtcatttga aattgggtaa cttgattgcc
     6361 atctacgatg acaacaagat cactatcgat ggtgctacca gtatctcatt cgatgaagat
     6421 gttgctaaga gatacgaagc ctacggttgg gaagttttgt acgtagaaaa tggtaacgaa
     6481 gatctagccg gtattgccaa ggctattgct caagctaagt tatccaagga caaaccaact
     6541 ttgatcaaaa tgaccacaac cattggttac ggttccttgc atgccggctc tcactctgtg
     6601 cacggtgccc cattgaaagc agatgatgtt aaacaactaa agagcaaatt cggtttcaac
     6661 ccagacaagt cctttgttgt tccacaagaa gtttacgacc actaccaaaa gacaatttta
     6721 aagccaggtg tcgaagccaa caacaagtgg aacaagttgt tcagcgaata ccaaaagaaa
     6781 ttcccagaat taggtgctga attggctaga agattgagcg gccaactacc cgcaaattgg
     6841 gaatctaagt tgccaactta caccgccaag gactctgccg tggccactag aaaattatca
     6901 gaaactgttc ttgaggatgt ttacaatcaa ttgccagagt tgattggtgg ttctgccgat
     6961 ttaacacctt ctaacttgac cagatggaag gaagcccttg acttccaacc tccttcttcc
     7021 ggttcaggta actactctgg tagatacatt aggtacggta ttagagaaca cgctatgggt
     7081 gccataatga acggtatttc agctttcggt gccaactaca aaccatacgg tggtactttc
     7141 ttgaacttcg tttcttatgc tgctggtgcc gttagattgt ccgctttgtc tggccaccca
     7201 gttatttggg ttgctacaca tgactctatc ggtgtcggtg aagatggtcc aacacatcaa
     7261 cctattgaaa ctttagcaca cttcagatcc ctaccaaaca ttcaagtttg gagaccagct
     7321 gatggtaacg aagtttctgc cgcctacaag aactctttag aatccaagca tactccaagt
     7381 atcattgctt tgtccagaca aaacttgcca caattggaag gtagctctat tgaaagcgct
     7441 tctaagggtg gttacgtact acaagatgtt gctaacccag atattatttt agtggctact
     7501 ggttccgaag tgtctttgag tgttgaagct gctaagactt tggccgcaaa gaacatcaag
     7561 gctcgtgttg tttctctacc agatttcttc acttttgaca aacaacccct agaatacaga
     7621 ctatcagtct taccagacaa cgttccaatc atgtctgttg aagttttggc taccacatgt
     7681 tggggcaaat acgctcatca atccttcggt attgacagat ttggtgcctc cggtaaggca
     7741 ccagaagtct tcaagttctt cggtttcacc ccagaaggtg ttgctgaaag agctcaaaag
     7801 accattgcat tctataaggg tgacaagcta atttctcctt tgaaaaaagc tttctaatct
     7861 agaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca
     7921 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     7981 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     8041 tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     8101 gagaaggttt tgggacgctc gaaggcttta atttgcaagc ttcgcagttt acactctcat
     8161 cgtcgtcgcg aataatagtc gaccttaagc atggtcatag ctgtttcctg tgtgaaattg
     8221 ttatccgctc acaattccac acaacatagg agccggaagc ataaagtgta aagcctgggg
     8281 tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8341 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8401 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8461 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8521 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8581 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8641 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8701 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8761 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8821 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8881 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8941 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9001 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9061 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9121 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9181 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9241 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9301 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9361 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9421 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9481 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9541 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9601 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9661 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9721 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9781 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9841 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9901 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9961 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10021 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10081 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10141 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10201 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10261 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10321 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10381 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
