LOCUS       sequence-168937-        3300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HRAS, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HRAS_WT
FEATURES             Location/Qualifiers
     source          1..3300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      43..631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     532..551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     785..802
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(961..988)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1080..1166)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1215..1237
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1229..1246
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1230..1246
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1262..1361
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1872..1971)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1988..2007)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1989..2007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2012..2028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2012..2028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2459..3250
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
       61 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      721 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      781 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      841 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      901 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
      961 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
     1021 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
     1081 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     1141 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct
     1201 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
     1261 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
     1321 atgctttttt ataatgccaa ctttgtacaa aaaagttggc atgacggaat ataagctggt
     1381 ggtggtgggc gccggcggtg tgggcaagag tgcgctgacc atccagctga tccagaacca
     1441 ttttgtggac gaatacgacc ccactataga ggattcctac cggaagcagg tggtcattga
     1501 tggggagacg tgcctgttgg acatcctgga taccgccggc caggaggagt acagcgccat
     1561 gcgggaccag tacatgcgca ccggggaggg cttcctgtgt gtgtttgcca tcaacaacac
     1621 caagtctttt gaggacatcc accagtacag ggagcagatc aaacgggtga aggactcgga
     1681 tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg gctgcacgca ctgtggaatc
     1741 tcggcaggct caggacctcg cccgaagcta cggcatcccc tacatcgaga cctcggccaa
     1801 gacccggcag ggcagccgct ctggctctag ctccagctcc gggaccctct gggacccccc
     1861 gggacccatg tgcccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1921 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1981 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2041 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2101 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2161 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2221 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2281 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2341 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2401 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     2461 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2521 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2581 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2641 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2701 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2761 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2821 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2881 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2941 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3001 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3061 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3121 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3181 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3241 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
//
