LOCUS       sequence-168944-        4557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PPP2R1A, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PPP2R1A_WT
FEATURES             Location/Qualifiers
     source          1..4557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     291..310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     544..561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(720..747)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(839..925)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     974..996
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     988..1005
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     989..1005
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1021..1120
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2888..2987)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3004..3023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3005..3023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3028..3044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3028..3044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3475..4266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4359..4557,1..390)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
       61 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      181 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      421 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      481 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      541 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      601 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      661 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
      721 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
      781 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
      841 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      901 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
      961 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     1021 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     1081 tgctttttta taatgccaac tttgtacaaa aaagttggca tggcggcggc cgacggcgac
     1141 gactcgctgt accccatcgc ggtgctcata gacgaactcc gcaatgagga cgttcagctt
     1201 cgcctcaaca gcatcaagaa gctgtccacc atcgccttgg cccttggggt tgaaaggacc
     1261 cgaagtgagc ttctgccttt ccttacagat accatctatg atgaagatga ggtcctcctg
     1321 gccctggcag aacagctggg aaccttcact accctggtgg gaggcccaga gtacgtgcac
     1381 tgcctgctgc caccgctgga gtcgctggcc acagtggagg agacagtggt gcgggacaag
     1441 gcagtggagt ccttacgggc catctcacac gagcactcgc cctctgacct ggaggcgcac
     1501 tttgtgccgc tagtgaagcg gctggcgggc ggcgactggt tcacctcccg cacctcggcc
     1561 tgcggcctct tctccgtctg ctacccccga gtgtccagtg ctgtgaaggc ggaacttcga
     1621 cagtacttcc ggaacctgtg ctcagatgac acccccatgg tgcggcgggc cgcagcctcc
     1681 aagctggggg agtttgccaa ggtgctggag ctggacaacg tcaagagtga gatcatcccc
     1741 atgttctcca acctggcctc tgacgagcag gactcggtgc ggctgctggc ggtggaggcg
     1801 tgcgtgaaca tcgcccagct tctgccccag gaggatctgg aggccctggt gatgcccact
     1861 ctgcgccagg ccgctgaaga caagtcctgg cgcgtccgct acatggtggc tgacaagttc
     1921 acagagctcc agaaagcagt ggggcctgag atcaccaaga cagacctggt ccctgccttc
     1981 cagaacctga tgaaagactg tgaggccgag gtgagggccg cagcctccca caaggtcaaa
     2041 gagttctgtg aaaacctctc agctgactgt cgggagaatg tgatcatgtc ccagatcttg
     2101 ccctgcatca aggagctggt gtccgatgcc aaccaacatg tcaagtctgc cctggcctca
     2161 gtcatcatgg gtctctctcc catcttgggc aaagacaaca ccatcgagca cctcttgccc
     2221 ctcttcctgg ctcagctgaa ggatgagtgc cctgaggtac ggctgaacat catctctaac
     2281 ctggactgtg tgaacgaggt gattggcatc cggcagctgt cccagtccct gctccctgcc
     2341 attgtggagc tggctgagga cgccaagtgg cgggtgcggc tggccatcat tgagtacatg
     2401 cccctcctgg ctggacagct gggagtggag ttctttgatg agaaacttaa ctccttgtgc
     2461 atggcctggc ttgtggatca tgtatatgcc atccgcgagg cagccaccag caacctgaag
     2521 aagctagtgg aaaagtttgg gaaggagtgg gcccatgcca caatcatccc caaggtcttg
     2581 gccatgtccg gagaccccaa ctacctgcac cgcatgacta cgctcttctg catcaatgtg
     2641 ctgtctgagg tctgtgggca ggacatcacc accaagcaca tgctacccac ggttctgcgc
     2701 atggctgggg acccggttgc caatgtccgc ttcaatgtgg ccaagtctct gcagaagata
     2761 gggcccatcc tggacaacag caccttgcag agtgaagtca agcccatcct agagaagctg
     2821 acccaggacc aggatgtgga cgtcaaatac tttgcccagg aggctctgac tgttctgtct
     2881 ctcgcctgcc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2941 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     3001 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     3061 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     3121 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     3181 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     3241 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     3301 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     3361 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     3421 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     3481 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     3541 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     3601 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     3661 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     3721 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     3781 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3841 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3901 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3961 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     4021 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     4081 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     4141 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     4201 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     4261 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     4321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccg
//
