LOCUS       sequence-168984-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  proximity-directed biotin labeling on the FRB domain of mTOR in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Lee SY, Lee H, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK, Lee C, Rhee HW
  TITLE     Proximity-Directed Labeling Reveals a New Rapamycin-Induced 
            Heterodimer of FKBP25 and FRB in Live Cells.
  JOURNAL   ACS Cent Sci. 2016 Aug 24;2(8):506-16. doi: 
            10.1021/acscentsci.6b00137. Epub 2016 Aug 12.
  PUBMED    27610411
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.6b00137"; journalName: "ACS Cent Sci"; date: 
            "2016-08-24- 24"; volume: "2"; issue: "8"; pages: "506-16"
COMMENT     Sequence Label: pCDNA5_FRB-pBirA-HA
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     83..107
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             216..494
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 gcgggggggg ggggggcata taagcaggag ctctccctat cagtgataga gatctcccta
       61 tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
      121 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cggactctag
      181 cgtttaaact taagcttggt accatggctt ctagaatcct ctggcatgag atgtggcatg
      241 aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg
      301 aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg aaggaaacat
      361 cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc aggaagtaca
      421 tgaaatcagg gaatgtcaag gacctcctcc aagcctggga cctctattat catgtgttcc
      481 gacgaatctc aaagactagt gaattcggat ccaaggacaa caccgtgccc ctgaagctga
      541 tcgccctgct ggccaacggc gagttccact ctggcgagca gctgggagag accctgggaa
      601 tgagcagagc cgccatcaac aagcacatcc agacactgag agactgggga gtggacgtgt
      661 tcaccgtgcc tggcaagggc tacagcctgc ctgagcctat ccagctgctg aacgccaagc
      721 agatcctggg acagctggat ggcggaagcg tggccgtgct gcctgtgatc gactccacca
      781 atcagtacct gctggacaga atcggagagc tgaagtccgg cgacgcctgc atcgccgagt
      841 accagcaggc tggcagagga ggcagaggac ggaagtggtt cagcccattc ggagccaacc
      901 tgtacctgtc catgttctgg agactggagc agggacctgc tgctgccatc ggactgaagt
      961 ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc tgggagccga caaggtgaag
     1021 agtgaagtgg cctaatgacc tgtacctcca ggaccgcaag ctggctggca tcctgggtgg
     1081 agctgacagg caagacaggc gatgccgctt cagatcgtga tcggagccgg aatcaacatg
     1141 ggcctgaaga ga
//
