LOCUS       sequence-168986-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed C-term tagged APEX2 on Sec61B in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of Proteins
            of Interest.
  JOURNAL   Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064.
  PUBMED    27184847
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064."
COMMENT     Sequence Label: pCDNA3_Sec61B-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..815)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(868..1155)
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     primer_bind     complement(1180..1199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1181..1199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgctgagct cttggcattt taggtgaact atagaatagg gccctctaga tgcatgctcg
       61 agtcattagg catcagcaaa cccaagctcg gaaagctttt ggtgagcctc agcgtaatca
      121 gcaaagaagg catcttcgtc cgctgcatat ttatcaacga gagggcggaa tacagggtca
      181 gacaaaagag ccttgtcaga aggtagctga aggagacctt ccttctcacc actcaacaac
      241 tccgtgaagt atgagttgtc gaaaataaga ggattagagg tccagggacc ctcaaatcca
      301 gaacgctcct tgtgtgcagc tccaatagtg tgacccccag atagagcaac gatatcttgg
      361 tcagtaagcc ccatagcttt gccaaacaca tctctcaaat ggtcagaacc cttagtggga
      421 tcgggcaagc gaccctctgg tggtggctca ggcttgtcct ctcttccagg gtggaatgga
      481 accttaggtc cacccgtgac ctcaacggca acaacgccag ccaactggta gaaatcggcg
      541 tagctcaaaa tagggaactc cgccttgagt ggctccaaaa gcctaacagc gatgtcaaga
      601 ccgttgttag cgctgtgagc cagttcggca gggtgcttga tggttccgaa gggtccaccg
      661 gtcttcgtgc ccttgtcaaa ggttccagca gagtggaatg ccaaacggag cattagagga
      721 gcgcatctct tctcagcgat gaagcctctg agcttcttct tcgccttctc aacggcgtcc
      781 tggtaatcag cactcacagt tgggtaagac tttccggtgc tgtccaggcc cagcaggggg
      841 ttgggggatg ggcttgcctg ctagcttcga acgagtgtac ttgccccaaa tgtgcaacat
      901 aaatacagaa gcgatgaaca gaagactcat aaccaatact ggaacagggc caactttgag
      961 cccaggtgaa tcttctgtgt agaatcgcca catccccccg gtgcctgccg aggttgtgcg
     1021 gcctgcactc cttgtcccac agctggcatt tttcctctgc cggacagtgg atcccgccgc
     1081 ccgggcggcc actgctttgc tgggagagcg ccctgaggat cccacgttag tgccactggg
     1141 ggtcggacca ggcatggtgg cggccgcaag cttgggtctc cctatagtga gtcgtattaa
     1201 tttcgataag ccagtaagca gtggggtca
//
