LOCUS       sequence-168987-        1172 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed C-term tagged APEX2 on Sec61B in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of Proteins
            of Interest.
  JOURNAL   Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064.
  PUBMED    27184847
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064."
COMMENT     Sequence Label: pCDNA3_Sec61B-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..1172
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..94
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             119..406
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     CDS             416..457
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cccaatggtg ggaaggtata tagcagagct ctctggctaa ctagagaacc cactgcttac
       61 tggcttatcg aaattaatac gactcactat agggagaccc aagcttgcgg ccgccaccat
      121 gcctggtccg acccccagtg gcactaacgt gggatcctca gggcgctctc ccagcaaagc
      181 agtggccgcc cgggcggcgg gatccactgt ccggcagagg aaaaatgcca gctgtgggac
      241 aaggagtgca ggccgcacaa cctcggcagg caccgggggg atgtggcgat tctacacaga
      301 agattcacct gggctcaaag ttggccctgt tccagtattg gttatgagtc ttctgttcat
      361 cgcttctgta tttatgttgc acatttgggg caagtacact cgttcgaagc tagcaggcaa
      421 gcccatcccc aaccccctgc tgggcctgga cagcaccgga aagtcttacc caactgtgag
      481 tgctgattac caggacgccg ttgagaaggc gaagaagaag ctcagaggct tcatcgctga
      541 gaagagatgc gctcctctaa tgctccgttt ggcattccac tctgctggaa cctttgacaa
      601 gggcacgaag accggtggac ccttcggaac catcaagcac cctgccgaac tggctcacag
      661 cgctaacaac ggtcttgaca tcgctgttag gcttttggag ccactcaagg cggagttccc
      721 tattttgagc tacgccgatt tctaccagtt ggctggcgtt gttgccgttg aggtcacggg
      781 tggacctaag gttccattcc accctggaag agaggacaag cctgagccac caccagaggg
      841 tcgcttgccc gatcccacta agggttctga ccatttgaga gatgtgtttg gcaaagctat
      901 ggggcttact gaccaagata tcgttgctct atctgggggt cacactattg gagctgcaca
      961 caaggagcgt tctggatttg agggtccctg gacctctaat cctcttattt tcgacaactc
     1021 atacttcacg gaattgttga atggtgaaaa aggaaggtct ccttcagcta ccttctgaca
     1081 aggctctttt gtctgaccct gtattccgcc ctctcgttga taaatatgca gcggacgaag
     1141 atgccttctt tgctgattac gctgaggctc ca
//
