LOCUS       sequence-168993-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed C-term tagged APEX2 on HMOX1 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of Proteins
            of Interest.
  JOURNAL   Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064.
  PUBMED    27184847
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064."
COMMENT     Sequence Label: pCDNA3_HMOX1-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             993..1034
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ccggggttgt gggaagctta taagcagagc tctctggcta actagagaac ccactgctta
       61 ctggcttatc gaaattaata cgactcacta tagggagacc caagcttgcg gccgccacca
      121 tggagcgtcc gcaacccgac agcatgcccc aggatttgtc agaggccctg aaggaggcca
      181 ccaaggaggt gcacacccag gcagagaatg ctgagttcat gaggaacttt cagaagggcc
      241 aggtgacccg agacggcttc aagctggtga tggcctccct gtaccacatc tatgtggccc
      301 tggaggagga gattgagcgc aacaaggaga gcccagtctt cgcccctgtc tacttcccag
      361 aagagctgca ccgcaaggct gccctggagc aggacctggc cttctggtac gggccccgct
      421 ggcaggaggt catcccctac acaccagcca tgcagcacta tgtgaagcgg ctccacgagg
      481 tggggcgcac agagcccgag ctgctggtgg cccacgccta cacccgctac ctgggtgacc
      541 tgtctggggg ccaggtgctc aaaaagattg cccagaaagc cctggacctg cccagctctg
      601 gcgagggcct ggccttcttc accttcccca acattgccag tgccaccaag ttcaagcagc
      661 tctaccgctc ccgcatgaac tccctggaga tgactcccgc agtcaggcag agggtgatag
      721 aagaggccaa gactgcgttc ctgctcaaca tccagctctt tgaggagttg caggagctgc
      781 tgacccatga caccaaggac cagagcccct cacgggcacc agggcttcgc cagcgggcca
      841 gcaacaaagt gcaagattct gcccccgtgg agactcccag agggaagccc ccactcaaca
      901 cccgctccca ggctccgctt ctccgatggg tccttacact cagctttctg gtggcgacag
      961 ttgctgtagg gctttatgcc atgaagctag caggcaagcc catccccaac cccctgctgg
     1021 gcctggacag caccggaaag tcttacccaa ctgtgagtgc tgattaccag gacgccgttg
     1081 aaaaggcgaa gaaaaagctc aaaggcttca tcgctgaaaa gaaatgcgct cc
//
