LOCUS       sequence-168999-        3711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GPR141 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GPR141_WT
FEATURES             Location/Qualifiers
     source          1..3711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1111..1210)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1227..1246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1228..1246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1251..1267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1251..1267)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1698..2489
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2582..3170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3071..3090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3324..3341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3500..3527)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3619..3705)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcctggc cacaatacct ccaggaattc ctcttgcgat cctatagtga
      241 caccccactt aatcagcctc tacttcatag tgcttattgg cgggctggtg ggtgtcattt
      301 ccattctttt cctcctggtg aaaatgaaca cccggtcagt gaccaccatg gcggtcatta
      361 acttggtggt ggtccacagc gtttttctgc tgacagtgcc atttcgcttg acctacctca
      421 tcaagaagac ttggatgttt gggctgccct tctgcaaatt tgtgagtgcc atgctgcaca
      481 tccacatgta cctcacgttc ctattctatg tggtgatcct ggtcaccaga tacctcatct
      541 tcttcaagtg caaagacaaa gtggaattct acagaaaact gcatgctgtg gctgccagtg
      601 ctggcatgtg gacgctggtg attgtcattg tggtacccct ggttgtctcc cggtatggaa
      661 tccatgagga atacaatgag gagcactgtt ttaaatttca caaagagctt gcttacacat
      721 atgtgaaaat catcaactat atgatagtca tttttgtcat agccgttgct gtgattctgt
      781 tggtcttcca ggtcttcatc attatgttga tggtgcagaa gctacgccac tctttactat
      841 cccaccagga gttctgggct cagctgaaaa acctattttt tataggggtc atccttgttt
      901 gtttccttcc ctaccagttc tttaggatct attacttgaa tgttgtgacg cattccaatg
      961 cctgtaacag caaggttgca ttttataacg aaatcttctt gagtgtaaca gcaattagct
     1021 gctatgattt gcttctcttt gtctttgggg gaagccattg gtttaagcaa aagataattg
     1081 gcttatggaa ttgtgttttg tgccgttgat tgccaacttt cttgtacaaa gttggcatta
     1141 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1201 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1261 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1321 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1381 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1441 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1501 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1561 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1621 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1681 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1741 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1801 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1861 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1921 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1981 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2041 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2101 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2161 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2221 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2281 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2341 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2401 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2461 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2521 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2581 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2641 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2701 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2761 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2821 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2881 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2941 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3001 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3061 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3121 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3181 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3241 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3301 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3361 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3421 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3481 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3541 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3601 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3661 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
