LOCUS       sequence-169001-        4515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAM200A ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FAM200A_WT
FEATURES             Location/Qualifiers
     source          1..4515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1915..2014)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2031..2050)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2032..2050)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2055..2071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2055..2071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2502..3293
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3386..3974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3875..3894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4128..4145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4304..4331)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4423..4509)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgactcct gaatcaaggg atactacaga tttgtctcca gggggtaccc
      241 aggagatgga aggcatcgtg atagtgaagg tggaggagga agatgaagaa gaccattttc
      301 aaaaggaaag aaacaaagta gagtcatcgc cacaagttct cagtcgctct acaactatga
      361 atgagagagc cttattgtca tcgtatttag ttgcatatag agtggcaaaa gagaaaatgg
      421 ctcacacagc ggctgaaaaa attatccttc cagcatgtat ggacatggta cggacaattt
      481 ttgatgacaa atcagctgat aaactaagaa ctatacctct tagtgataat acaatatctc
      541 gtcgaatctg tacgattgca aaacatttgg aagcaatgct tattacacgg ctgcagtccg
      601 gtatagactt tgcaatccaa ctcgatgaga gcactgatat tgcaagttgt cccacactct
      661 tggtttatgt cagatatgtg tggcaagatg attttgtaga ggatctctta tgttgtttaa
      721 atttaaattc acatataact ggattagatt tatttactga attagaaaac tgccttcttg
      781 gtcagtataa attaaactgg aaacattgta aaggaatttc aagtgatgga acagcaaata
      841 tgaccggaaa acacagcaga cttactgaaa aattgttaga agcaacccac aacaatgctg
      901 tttggaatca ctgttttatt catcgagaag ctttggtatc caaagaaatt tcaccaagtc
      961 tgatggatgt attgaaaaat gcagtgaaaa ctgttaattt tattaaagga agctcactga
     1021 atagccgact tctcgaaata ttttgttcag agattggagt gaaccacacc cacttattgt
     1081 ttcatacaga agttcgttgg ctttctcaag gaaaagtatt gagcagagta tatgaactca
     1141 ggaacgagat ttacattttt ctcgttgaaa agcaatctca tttggcaaat atttttgaag
     1201 acgacatttg ggtaacaaaa ttggcatatt taagtgatat ttttggcatt cttaatgaat
     1261 taagcctgaa aatgcagggg aaaaacaatg atatatttca gtatcttgaa catattctag
     1321 gattccaaaa gacgttatta ttgtggcaag caagacttaa aagtaaccgc cctagctact
     1381 atatgtttcc aacattattg caacacatcg aagagaacat tattaatgaa gactgcttaa
     1441 aagaaataaa attagagata ttgttgcatc tcacttcttt gtctcaaact tttaattatt
     1501 actttccgga agagaaattt gaatcattaa aggaaaatat ttggatgaaa gatccatttg
     1561 cttttcaaaa cccagaatca ataattgagt taaacttgga gcctgaagaa gagaatgaat
     1621 tattgcagct cagttcatca ttcacactaa agaattatta taagatatta agtttatcag
     1681 cattttggat taagattaaa gatgactttc cactgctaag taggaagagt atattgctgt
     1741 tactaccatt cacaactaca tatttgtgtg aactaggatt ttcaatcttg acacggttaa
     1801 aaacaaagaa gagaaatagg ctcaatagtg caccagatat gcgggtagca ttatcttcat
     1861 gtgttcctga ctggaaggaa cttatgaaca gacaagcaca cccatcacat tgattgccaa
     1921 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1981 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2041 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2101 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     2161 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2221 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2281 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2341 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2401 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2461 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2521 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2581 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2641 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2701 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2761 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2821 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2881 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2941 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3001 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3061 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3121 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3181 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3241 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3301 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4261 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4321 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4381 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4441 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4501 ttttatttga tgcct
//
