LOCUS       sequence-169007-        4653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ELF1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ELF1_WT
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2053..2152)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2169..2188)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2170..2188)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2193..2209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2193..2209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2640..3431
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3524..4112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4013..4032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4266..4283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4442..4469)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4561..4647)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggctgct gttgtccaac agaacgacct agtatttgaa tttgctagta
      241 acgtcatgga ggatgaacga cagcttggtg atccagctat ttttcctgcc gtaattgtgg
      301 aacatgttcc tggtgctgat attctcaata gttatgccgg tctagcctgt gtggaagagc
      361 ccaatgacat gattactgag agttcactgg atgttgctga agaagaaatc atagacgatg
      421 atgatgatga catcaccctt acagttgaag cttcttgtca tgacggggat gaaacaattg
      481 aaactattga ggctgctgag gcactcctca atatggattc ccctggccct atgctggatg
      541 aaaaacgaat aaataataat atatttagtt cacctgaaga tgacatggtt gttgccccag
      601 tcacccatgt gtccgtcaca ttagatggga ttcctgaagt gatggaaaca cagcaggtgc
      661 aagaaaaata tgcagactca ccgggagcct catcaccaga acagcctaag aggaaaaaag
      721 gaagaaaaac taaaccacca cgaccagatt ccccagccac tacgccaaat atatctgtga
      781 agaagaaaaa caaagatgga aagggaaaca caatttatct ttgggagttt ttactggcac
      841 tgctccagga caaggctact tgtcctaaat acatcaagtg gacccagcga gagaaaggca
      901 tttttaaatt ggtggattct aaagcagtgt ccaggttgtg ggggaagcac aaaaacaaac
      961 ctgatatgaa ttatgagacc atgggaagag cactcaggta ctattaccaa aggggtattc
     1021 tggcaaaagt ggaaggtcag cgcttggtgt atcagtttaa agaaatgcca aaagatctta
     1081 tatatataaa tgatgaggat ccaagttcca gcatagagtc ttcagatcca tcactatctt
     1141 catcagccac ttcaaatagg aatcaaacca gccggtcgag agtatcttca agtccagggg
     1201 taaaaggagg agccactaca gttctaaaac cagggaattc taaagctgca aaacccaaag
     1261 atcctgtgga agttgcacaa ccatcagaag ttttgaggac agtgcagccc acgcagtctc
     1321 catatcctac ccagctcttc cggactgttc atgtagtaca gccagtacag gctgtcccag
     1381 agggagaagc agctagaacc agtaccatgc aggatgaaac attaaattct tccgttcaga
     1441 gtattaggac tatacaggct ccaacccaag ttccagtggt tgtgtctcct aggaatcagc
     1501 agttgcatac agtaacactc caaacagtgc cactcacaac agttatagcc agcacagatc
     1561 catcagcagg tactggatct cagaagttta ttttacaagc cattccatca tcacagccca
     1621 tgacagtact gaaagaaaat gtcatgctgc agtcacaaaa ggcgggctct cctccttcaa
     1681 ttgtcttggg ccctgcccag gttcagcagg tccttactag caatgttcag accatttgca
     1741 atggaaccgt cagtgtggct tcctctccat ccttcagtgc tactgcacct gtggtgacct
     1801 tttctcctcg cagttcacag ctggttgctc acccacctgg cactgtaatc acttcagtta
     1861 tcaaaactca agaaacaaaa actcttacac aggaagtaga gaaaaaggaa tctgaagatc
     1921 atttgaaaga gaacactgag aaaacggagc agcagccaca gccttatgtg atggtagtgt
     1981 ccagttccaa tggatttact tctcaggtag ctatgaaaca aaacgaactg ctggaaccca
     2041 actctttttg attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
     2101 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     2161 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     2221 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
     2281 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     2341 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     2401 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2461 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     2521 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2581 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     2641 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     2701 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     2761 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2821 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2881 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2941 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     3001 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     3061 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     3121 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     3181 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     3241 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     3301 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     3361 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     3421 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     3481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3661 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4141 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4201 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4261 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4321 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4381 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     4441 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4501 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4561 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4621 ttccgactga gcctttcgtt ttatttgatg cct
//
