LOCUS       sequence-169013-        4368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing OMA1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_OMA1_WT
FEATURES             Location/Qualifiers
     source          1..4368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1768..1867)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1884..1903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1885..1903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1908..1924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1908..1924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2355..3146
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3239..3827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3728..3747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3981..3998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4157..4184)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4276..4362)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgagcttc atctgtggat tgcagtctgc tgctagaaac catgttttct
      241 tccgatttaa ttcactgtct aactggagaa aatgtaacac attagcatcc acctcacggg
      301 gctgtcatca agtacaagtt aaccatatag taaataagta tcagggactg ggagtaaatc
      361 agtgtgacag gtggagtttt ctgcctggaa actttcattt ttatagtact tttaacaaca
      421 aaagaacagg aggcctctca agtaccaaaa gtaaggaaat ttggaggatt accagcaaat
      481 gtactgtatg gaatgatgct ttttcaagac agctgctaat aaaagaagtt acagcagtcc
      541 ctagtctgtc agtattgcat cctctaagcc ctgcttccat aagagctatt aggaatttcc
      601 atacttctcc acggtttcaa gctgctccgg ttcctctctt gttgatgatt cttaaaccag
      661 tacagaagtt atttgcaatc attgtaggca ggggcataag gaaatggtgg caggcacttc
      721 ctcctaacaa gaaggaagta gttaaagaaa atataaggaa gaataaatgg aagctattcc
      781 ttggtttgag tagttttgga ttgctctttg tggtgtttta ttttactcac ctggaagtaa
      841 gtccaatcac aggaaggagc aagctactat tattggggaa agaacagttc agacttttat
      901 cggaactgga atatgaagca tggatggaag aatttaaaaa tgatatgcta actgagaaag
      961 atgcccgata cctggctgtt aaagaagtgc tttgtcatct aattgaatgc aataaagatg
     1021 ttccagggat ctctcagatc aattgggtta ttcatgtggt tgattcccca attattaatg
     1081 ccttcgtgct tccaaatgga caaatgtttg ttttcactgg atttttaaat agtgtaaccg
     1141 atattcatca actttctttc cttctgggcc atgaaatagc acatgcagta cttgggcatg
     1201 ctgcagaaaa ggctggcatg gttcatttgt tggatttcct aggtatgatt ttcctcacaa
     1261 tgatttgggc catttgtcct cgagatagct tggcactttt gtgccagtgg atacagtcta
     1321 aattgcagga gtatatgttt aatagaccat acagcagaaa attggaggcc gaagctgaca
     1381 aaattggact actgcttgct gcaaaggctt gtgcagacat aagagccagt tcagtgtttt
     1441 ggcagcaaat ggagttcgtt gatagcctgc atggccaacc caagatgcca gaatggttat
     1501 ctacacaccc ttctcatggc aatcgagttg agtacttgga tagacttata cctcaggctc
     1561 tcaaaattag agagatgtgt aattgtccac cactgtctaa tccagaccct cgattactat
     1621 tcaaactcag cacgaagcat tttcttgaag aatcagagaa agaagaccta aatatcacga
     1681 agaaacagaa aatggatact cttcctattc aaaaacagga gcaaatacca ttaacataca
     1741 tagttgagaa aagaacgggc agttgattgc caactttctt gtacaaagtt ggcattataa
     1801 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1861 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1921 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1981 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2041 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2101 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2161 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2221 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2281 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2341 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2401 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2461 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2521 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2581 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2641 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2701 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2761 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2821 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2881 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2941 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3001 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3061 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3121 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3181 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3901 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3961 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4081 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4141 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4201 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4261 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4321 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
