LOCUS       sequence-169017-        4359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KPNA3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KPNA3_WT
FEATURES             Location/Qualifiers
     source          1..4359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1759..1858)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1875..1894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1876..1894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1899..1915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1899..1915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2346..3137
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3230..3818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3719..3738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3972..3989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4148..4175)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4267..4353)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggccgag aaccccagct tggagaacca ccgcatcaag agcttcaaga
      241 acaagggccg cgatgtggaa acaatgcgaa gacatagaaa tgaagtgaca gtggaactgc
      301 ggaagaacaa aagagatgaa cacttattga aaaagagaaa tgttccccaa gaagaaagtc
      361 tagaagattc agatgttgat gctgatttta aagcacaaaa tgtaacccta gaagctatat
      421 tgcagaatgc cacaagtgat aacccagtgg tccaattgag tgctgtccag gcagcaagaa
      481 aactgttatc cagtgacaga aatccaccga ttgatgactt aataaaatct gggattttac
      541 caattctagt caaatgtcta gaaagggatg ataatccttc attacagttt gaagctgctt
      601 gggcattaac taacatagca tcaggaactt ctgcacagac tcaagatatt gtgcagtcta
      661 atgcagtacc tctttttctg agacttcttc gttcaccaca tcagaatgtt tgtgaacaag
      721 cagtatgggc tttgggaaac attataggtg atggtcctca atgtagagat tatgtcatat
      781 cactgggagt tgtcaaacct cttctgtcct tcatcagtcc ctccatcccc atcaccttcc
      841 ttcggaacgt cacatgggtc attgtcaatc tctgcaggaa taaggatccc ccaccgccta
      901 tggagacagt tcaggagatt ttgccagctt tatgtgtcct catataccat acagatataa
      961 acattcttgt agacactgtt tgggctctgt catacttgac agatggaggt aatgaacaga
     1021 tacagatggt tattgattca ggagttgtgc cctttcttgt gccccttctg agccatcagg
     1081 aagtcaaagt tcaaacagca gccctcagag cagttggcaa catagtgact ggcaccgacg
     1141 agcagaccca ggttgttctc aattgtgatg tcctgtcaca cttccaaaat ctcttatcac
     1201 acccaaaaga gaagataaat aaggaagcag tgtggttcct ttccaacata acagcaggca
     1261 accagcaaca agttcaagct gtaatagatg ctggattaat tcctatgata attcatcagc
     1321 ttgctaaggg ggactttgga acacaaaaag aagctgcttg ggcaatcagc aacttaacaa
     1381 taagtggcag aaaagatcag gttgagtacc ttgtacagca ggatgtaata ccaccgttct
     1441 gtaatttact gtcagtgaaa gattctcaag tggttcaggt ggttctagat ggtctaaaaa
     1501 acattctgat aatggccggt gatgaagcaa gcacaatagc tgaaataata gaggaatgtg
     1561 gaggtttgga gaaaattgaa gttttacagc aacatgaaaa tgaagacata tataaattag
     1621 catttgaaat catagatcag tatttctctg gtgatgatat tgatgaagat ccctgcctca
     1681 ttcctgaagc aacacaagga ggtacctaca attttgatcc aacagccaac cttcaaacaa
     1741 aagaatttaa tttttgattg ccaactttct tgtacaaagt tggcattata agaaagcatt
     1801 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1861 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1921 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1981 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2041 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2101 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2161 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2221 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2281 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2341 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2401 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2461 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2521 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2581 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2641 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2701 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2761 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2821 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2881 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2941 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3001 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3061 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3121 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3181 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4021 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4081 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4141 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4201 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4261 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4321 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
