LOCUS       sequence-169019-        5331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAT6, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAT6_WT
FEATURES             Location/Qualifiers
     source          1..5331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(933..960)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1052..1138)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1187..1209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1201..1218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1202..1218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1234..1333
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3875..3974)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3991..4010)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3992..4010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4015..4031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4015..4031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4462..5253
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
      901 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
      961 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     1021 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     1081 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     1141 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     1201 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
     1261 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
     1321 aaaaaagttg gcatgtctct gtggggtctg gtctccaaga tgcccccaga aaaagtgcag
     1381 cggctctatg tcgactttcc ccaacacctg cggcatcttc tgggtgactg gctggagagc
     1441 cagccctggg agttcctggt cggctccgac gccttctgct gcaacttggc tagtgcccta
     1501 ctttcagaca ctgtccagca ccttcaggcc tcggtgggag agcaggggga ggggagcacc
     1561 atcttgcaac acatcagcac ccttgagagc atatatcaga gggaccccct gaagctggtg
     1621 gccactttca gacaaatact tcaaggagag aaaaaagctg ttatggaaca gttccgccac
     1681 ttgccaatgc ctttccactg gaagcaggaa gaactcaagt ttaagacagg cttgcggagg
     1741 ctgcagcacc gagtagggga gatccacctt ctccgagaag ccctgcagaa gggggctgag
     1801 gctggccaag tgtctctgca cagcttgata gaaactcctg ctaatgggac tgggccaagt
     1861 gaggccctgg ccatgctact gcaggagacc actggagagc tagaggcagc caaagcccta
     1921 gtgctgaaga ggatccagat ttggaaacgg cagcagcagc tggcagggaa tggcgcaccg
     1981 tttgaggaga gcctggcccc actccaggag aggtgtgaaa gcctggtgga catttattcc
     2041 cagctacagc aggaggtagg ggcggctggt ggggagcttg agcccaagac ccgggcatcg
     2101 ctgactggcc ggctggatga agtcctgaga accctcgtca ccagttgctt cctggtggag
     2161 aagcagcccc cccaggtact gaagactcag accaagttcc aggctggagt tcgattcctg
     2221 ttgggcttga ggttcctggg ggccccagcc aagcctccgc tggtcagggc cgacatggtg
     2281 acagagaagc aggcgcggga gctgagtgtg cctcagggtc ctggggctgg agcagaaagc
     2341 actggagaaa tcatcaacaa cactgtgccc ttggagaaca gcattcctgg gaactgctgc
     2401 tctgccctgt tcaagaacct gcttctcaag aagatcaagc ggtgtgagcg gaagggcact
     2461 gagtctgtca cagaggagaa gtgcgctgtg ctcttctctg ccagcttcac acttggcccc
     2521 ggcaaactcc ccatccagct ccaggccctg tctctgcccc tggtggtcat cgtccatggc
     2581 aaccaagaca acaatgccaa agccactatc ctgtgggaca atgccttctc tgagatggac
     2641 cgcgtgccct ttgtggtggc tgagcgggtg ccctgggaga agatgtgtga aactctgaac
     2701 ctgaagttca tggctgaggt ggggaccaac cgggggctgc tcccagagca cttcctcttc
     2761 ctggcccaga agatcttcaa tgacaacagc ctcagtatgg aggccttcca gcaccgttct
     2821 gtgtcctggt cgcagttcaa caaggagatc ctgctgggcc gtggcttcac cttttggcag
     2881 tggtttgatg gtgtcctgga cctcaccaaa cgctgtctcc ggagctactg gtctgaccgg
     2941 ctgatcattg gcttcatcag caaacagtac gttactagcc ttcttctcaa tgagcccgac
     3001 ggaacctttc tcctccgctt cagcgactca gagattgggg gcatcaccat tgcccatgtc
     3061 atccggggcc aggatggctc tccacagata gagaacatcc agccattctc tgccaaagac
     3121 ctgtccattc gctcactggg ggaccgaatc cgggatcttg ctcagctcaa aaatctctat
     3181 cccaagaagc ccaaggatga ggctttccgg agccactaca agcctgaaca gatgggtaag
     3241 gatggcaggg gttatgtccc agctaccatc aagatgaccg tggaaaggga ccaaccactt
     3301 cctaccccag agctccagat gcctaccatg gtgccttctt atgaccttgg aatggcccct
     3361 gattcctcca tgagcatgca gcttggccca gatatggtgc cccaggtgta cccaccacac
     3421 tctcactcca tccccccgta tcaaggcctc tccccagaag aatcagtcaa cgtgttgtca
     3481 gccttccagg agcctcacct gcagatgccc cccagcctgg gccagatgag cctgcccttt
     3541 gaccagcctc acccccaggg cctgctgccg tgccagcctc aggagcatgc tgtgtccagc
     3601 cctgaccccc tgctctgctc agatgtgacc atggtggaag acagctgcct gagccagcca
     3661 gtgacagcgt ttcctcaggg cacttggatt ggtgaagaca tattccctcc tctgctgcct
     3721 cccactgaac aggacctcac taagcttctc ctggaggggc aaggggagtc ggggggaggg
     3781 tccttggggg cacagcccct cctgcagccc tcccactatg ggcaatctgg gatctcaatg
     3841 tcccacatgg acctaagggc caaccccagt tggtgcccaa ctttcttgta caaagttggc
     3901 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     3961 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     4021 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     4081 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
     4141 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     4201 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     4261 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     4321 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     4381 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     4441 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     4501 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     4561 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     4621 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     4681 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     4741 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     4801 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     4861 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     4921 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     4981 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     5041 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     5101 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     5161 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     5221 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     5281 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa g
//
