LOCUS       sequence-169020-        6561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2_WT
FEATURES             Location/Qualifiers
     source          1..6561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3961..4060)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4077..4096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4078..4096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4101..4117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4101..4117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4548..5339
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5432..6020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5921..5940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6174..6191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6350..6377)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6469..6555)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggagctg gcggccttgt gccgctgggg gctcctcctc gccctcttgc
      241 cccccggagc cgcgagcacc caagtgtgca ccggcacaga catgaagctg cggctccctg
      301 ccagtcccga gacccacctg gacatgctcc gccacctcta ccagggctgc caggtggtgc
      361 agggaaacct ggaactcacc tacctgccca ccaatgccag cctgtccttc ctgcaggata
      421 tccaggaggt gcagggctac gtgctcatcg ctcacaacca agtgaggcag gtcccactgc
      481 agaggctgcg gattgtgcga ggcacccagc tctttgagga caactatgcc ctggccgtgc
      541 tagacaatgg agacccgctg aacaatacca cccctgtcac aggggcctcc ccaggaggcc
      601 tgcgggagct gcagcttcga agcctcacag agatcttgaa aggaggggtc ttgatccagc
      661 ggaaccccca gctctgctac caggacacga ttttgtggaa ggacatcttc cacaagaaca
      721 accagctggc tctcacactg atagacacca accgctctcg ggcctgccac ccctgttctc
      781 cgatgtgtaa gggctcccgc tgctggggag agagttctga ggattgtcag agcctgacgc
      841 gcactgtctg tgccggtggc tgtgcccgct gcaaggggcc actgcccact gactgctgcc
      901 atgagcagtg tgctgccggc tgcacgggcc ccaagcactc tgactgcctg gcctgcctcc
      961 acttcaacca cagtggcatc tgtgagctgc actgcccagc cctggtcacc tacaacacag
     1021 acacgtttga gtccatgccc aatcccgagg gccggtatac attcggcgcc agctgtgtga
     1081 ctgcctgtcc ctacaactac ctttctacgg acgtgggatc ctgcaccctc gtctgccccc
     1141 tgcacaacca agaggtgaca gcagaggatg gaacacagcg gtgtgagaag tgcagcaagc
     1201 cctgtgcccg agtgtgctat ggtctgggca tggagcactt gcgagaggtg agggcagtta
     1261 ccagtgccaa tatccaggag tttgctggct gcaagaagat ctttgggagc ctggcatttc
     1321 tgccggagag ctttgatggg gacccagcct ccaacactgc cccgctccag ccagagcagc
     1381 tccaagtgtt tgagactctg gaagagatca caggttacct atacatctca gcatggccgg
     1441 acagcctgcc tgacctcagc gtcttccaga acctgcaagt aatccgggga cgaattctgc
     1501 acaatggcgc ctactcgctg accctgcaag ggctgggcat cagctggctg gggctgcgct
     1561 cactgaggga actgggcagt ggactggccc tcatccacca taacacccac ctctgcttcg
     1621 tgcacacggt gccctgggac cagctctttc ggaacccgca ccaagctctg ctccacactg
     1681 ccaaccggcc agaggacgag tgtgtgggcg agggcctggc ctgccaccag ctgtgcgccc
     1741 gagggcactg ctggggtcca gggcccaccc agtgtgtcaa ctgcagccag ttccttcggg
     1801 gccaggagtg cgtggaggaa tgccgagtac tgcaggggct ccccagggag tatgtgaatg
     1861 ccaggcactg tttgccgtgc caccctgagt gtcagcccca gaatggctca gtgacctgtt
     1921 ttggaccgga ggctgaccag tgtgtggcct gtgcccacta taaggaccct cccttctgcg
     1981 tggcccgctg ccccagcggt gtgaaacctg acctctccta catgcccatc tggaagtttc
     2041 cagatgagga gggcgcatgc cagccttgcc ccatcaactg cacccactcc tgtgtggacc
     2101 tggatgacaa gggctgcccc gccgagcaga gagccagccc tctgacgtcc atcatctctg
     2161 cggtggttgg cattctgctg gtcgtggtct tgggggtggt ctttgggatc ctcatcaagc
     2221 gacggcagca gaagatccgg aagtacacga tgcggagact gctgcaggaa acggagctgg
     2281 tggagccgct gacacctagc ggagcgatgc ccaaccaggc gcagatgcgg atcctgaaag
     2341 agacggagct gaggaaggtg aaggtgcttg gatctggcgc ttttggcaca gtctacaagg
     2401 gcatctggat ccctgatggg gagaatgtga aaattccagt ggccatcaaa gtgttgaggg
     2461 aaaacacatc ccccaaagcc aacaaagaaa tcttagacga agcatacgtg atggctggtg
     2521 tgggctcccc atatgtctcc cgccttctgg gcatctgcct gacatccacg gtgcagctgg
     2581 tgacacagct tatgccctat ggctgcctct tagaccatgt ccgggaaaac cgcggacgcc
     2641 tgggctccca ggacctgctg aactggtgta tgcagattgc caaggggatg agctacctgg
     2701 aggatgtgcg gctcgtacac agggacttgg ccgctcggaa cgtgctggtc aagagtccca
     2761 accatgtcaa aattacagac ttcgggctgg ctcggctgct ggacattgac gagacagagt
     2821 accatgcaga tgggggcaag gtgcccatca agtggatggc gctggagtcc attctccgcc
     2881 ggcggttcac ccaccagagt gatgtgtgga gttatggtgt gactgtgtgg gagctgatga
     2941 cttttggggc caaaccttac gatgggatcc cagcccggga gatccctgac ctgctggaaa
     3001 agggggagcg gctgccccag ccccccatct gcaccattga tgtctacatg atcatggtca
     3061 aatgttggat gattgactct gaatgtcggc caagattccg ggagttggtg tctgaattct
     3121 cccgcatggc cagggacccc cagcgctttg tggtcatcca gaatgaggac ttgggcccag
     3181 ccagtccctt ggacagcacc ttctaccgct cactgctgga ggacgatgac atgggggacc
     3241 tggtggatgc tgaggagtat ctggtacccc agcagggctt cttctgtcca gaccctgccc
     3301 cgggcgctgg gggcatggtc caccacaggc accgcagctc atctaccagg agtggcggtg
     3361 gggacctgac actagggctg gagccctctg aagaggaggc ccccaggtct ccactggcac
     3421 cctccgaagg ggctggctcc gatgtatttg atggtgacct gggaatgggg gcagccaagg
     3481 ggctgcaaag cctccccaca catgacccca gccctctaca gcggtacagt gaggacccca
     3541 cagtacccct gccctctgag actgatggct acgttgcccc cctgacctgc agcccccagc
     3601 ctgaatatgt gaaccagcca gatgttcggc cccagccccc ttcgccccga gagggccctc
     3661 tgcctgctgc ccgacctgct ggtgccactc tggaaaggcc caagactctc tccccaggga
     3721 agaatggggt cgtcaaagac gtttttgcct ttgggggtgc cgtggagaac cccgagtact
     3781 tgacacccca gggaggagct gcccctcagc cccaccctcc tcctgccttc agcccagcct
     3841 tcgacaacct ctattactgg gaccaggacc caccagagcg gggggctcca cccagcacct
     3901 tcaaagggac acctacggca gagaacccag agtacctggg tctggacgtg ccagtgtgat
     3961 tgccaacttt cttgtacaaa gttgtcatta taagaaagca ttgcttatca atttgttgca
     4021 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     4081 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     4141 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     4201 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     4261 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     4321 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     4381 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     4441 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     4501 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     4561 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     4621 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     4681 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     4741 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     4801 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     4861 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     4921 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     4981 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     5041 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     5101 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     5161 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     5221 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     5281 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     5341 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     5401 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6241 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6301 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     6361 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     6421 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     6481 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     6541 ctttcgtttt atttgatgcc t
//
