LOCUS       sequence-169023-        4131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WIPI1 ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WIPI1_WT
FEATURES             Location/Qualifiers
     source          1..4131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1531..1630)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1647..1666)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1648..1666)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1671..1687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1671..1687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2118..2909
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3002..3590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3491..3510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3744..3761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3920..3947)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4039..4125)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggaggccgag gccgcggacg ctcccccggg cggggttgag tcggcgctca
      241 gctgcttctc tttcaaccag gactgcacat ccctagcaat tggaactaaa gccgggtata
      301 agctgttttc tctgagttct gtggagcagc tggatcaagt ccacggaagc aatgaaatcc
      361 cggacgtcta catcgtggag cgcctcttct ccagcagcct ggtggtggta gtcagtcaca
      421 caaaaccacg gcagatgaac gtgtatcact tcaagaaagg cacagagatc tgtaattaca
      481 gctactccag caacatcttg tccataaggc tgaaccggca aaggctgctg gtttgcctag
      541 aagagtccat ttatattcac aacattaaag acatgaagct gttgaagacc ctcctggata
      601 ttcctgcaaa cccaacaggt ctatgtgctc tctctatcaa ccattccaat tcttacctgg
      661 cctatcctgg aagcctgact tcaggggaga ttgtgcttta tgatggaaac tccctgaaaa
      721 cagtctgcac tattgctgcc catgagggaa cactagctgc catcaccttc aatgcctcag
      781 gctccaaact agcaagtgcg tctgaaaaag gcacagtcat ccgggtgttc tctgtccctg
      841 atgggcaaaa gctctatgag ttccggagag ggatgaaaag gtatgtgaca atcagctctc
      901 tagtgttcag tatggattca caattcctct gcgcctccag taacaccgag acggtacaca
      961 tcttcaagct ggaacaggtc accaacagtc gaccagaaga gccttcgacc tggagtggct
     1021 acatgggaaa gatgtttatg gctgctacca actacctccc tacccaggtg tcagacatga
     1081 tgcatcagga cagggctttt gccactgcac gcttgaactt ctccggacag aggaacatct
     1141 gtaccctctc aacgatccag aagttgccac ggctgctagt tgcgtcatcc agtggacacc
     1201 tttatatgta caatttggat cctcaggatg gaggagagtg tgtcttaatc aaaacccaca
     1261 gcttgcttgg ctcaggaaca acagaagaga ataaagaaaa tgacctcaga ccttccttac
     1321 ctcagtctta tgcagcgacc gtagccagac caagtgcatc ttcagcctcc acggtgccag
     1381 gttattctga ggacggcggg gcgctgcgag gagaagttat tcctgaacat gagtttgcga
     1441 cgggaccagt gtgtcttgat gatgagaatg agtttcctcc tataatcttg tgccgtggaa
     1501 atcagaaggg caaaacgaag cagtcatgat tgccaacttt cttgtacaaa gttggcatta
     1561 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1621 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1681 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1741 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1801 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1861 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1921 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1981 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2041 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2101 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2161 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2221 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2281 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2341 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2401 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2461 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2521 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2581 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2641 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2701 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2761 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2821 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2881 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2941 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3901 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3961 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4021 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4081 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
