LOCUS       sequence-169030-        3510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing U2AF1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_U2AF1_WT
FEATURES             Location/Qualifiers
     source          1..3510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(941..968)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1060..1146)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1195..1217
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1209..1226
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1210..1226
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1242..1341
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2062..2161)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2178..2197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2179..2197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2202..2218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2202..2218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2649..3440
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      901 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
      961 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     1021 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     1081 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     1141 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     1201 cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt
     1261 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
     1321 ctttgtacaa aaaagttggc atggcggagt atctggcctc catcttcggc accgagaaag
     1381 acaaagtcaa ctgttcattt tatttcaaaa ttggagcatg tcgtcatgga gacaggtgct
     1441 ctcggttgca caataaaccg acgtttagcc agaccattgc cctcttgaac atttaccgta
     1501 accctcaaaa ctcttcccag tctgctgacg gtttgcgctg tgccgtgagc gatgtggaga
     1561 tgcaggaaca ctatgatgag ttttttgagg aggtttttac agaaatggag gagaagtatg
     1621 gggaagtaga ggagatgaac gtctgtgaca acctgggaga ccacctggtg gggaacgtgt
     1681 acgtcaagtt tcgccgtgag gaagatgcgg aaaaggctgt gattgacttg aataaccgtt
     1741 ggtttaatgg acagccgatc cacgccgagc tgtcacccgt gacggacttc agagaagcct
     1801 gctgccgtca gtatgagatg ggagaatgca cacgaggcgg cttctgcaac ttcatgcatt
     1861 tgaagcccat ttccagagag ctgcggcggg agctgtatgg ccgccgtcgc aagaagcata
     1921 gatcaagatc ccgatcccgg gagcgtcgtt ctcggtctag agaccgtggt cgtggcggtg
     1981 gcggtggcgg tggtggaggt ggcggcggac gggagcgtga caggaggcgg tcgagagatc
     2041 gtgaaagatc tgggcgattc tgcccaactt tcttgtacaa agttggcatt ataagaaagc
     2101 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2161 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     2221 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2281 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     2341 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     2401 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2461 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2521 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2581 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2641 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2701 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2761 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2821 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2881 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2941 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3001 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3061 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3121 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3181 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3241 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3301 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3361 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3421 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     3481 acgcgtcgtt ccactgagcg tcagaccccg
//
