LOCUS       sequence-169037-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM10, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RBM10_WT
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     520..539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     773..790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(949..976)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1068..1154)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1203..1225
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1217..1234
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1218..1234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1250..1349
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2086..2103)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(2702..2713)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4140..4239)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4256..4275)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4257..4275)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4280..4296)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4280..4296)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4727..5518
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       61 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      661 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      721 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      781 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      841 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      901 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
      961 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     1021 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     1081 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     1141 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     1201 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
     1261 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
     1321 aatgccaact ttgtacaaaa aagttggcat ggagtatgaa agacgtggtg gtcgtggtga
     1381 caggactggc cgctatggag ccactgaccg ctcgcaggat gatggtgggg agaaccgcag
     1441 ccgagaccac gactaccggg acatggacta ccgttcatat cctcgcgagt atggcagcca
     1501 ggagggcaag catgactatg acgactcatc tgaggagcag agtgcggagg attcctacga
     1561 ggcctccccg ggctccgaga ctcagcgtag gcggcggcgg cggcacaggc acagccccac
     1621 cggcccgcca ggcttccccc gagacggcga ctatcgggac caggactatc ggaccgagca
     1681 aggggaggag gaggaggagg aggaggatga ggaggaggag gagaaggcca gtaacatcgt
     1741 catgctgagg atgctgccac aggcagccac tgaggatgac atccgtggcc agctgcagtc
     1801 gcacggcgtg caagcacggg aggttcggct gatgcggaac aaatcttcag gtcagagccg
     1861 gggcttcgcc ttcgtcgagt ttagtcactt gcaggacgct acacgatgga tggaagccaa
     1921 tcagcactcc ctcaacatcc tgggccagaa ggtgtcgatg cactacagtg accccaagcc
     1981 caagatcaat gaggactggc tgtgcaataa gtgtggcgtc cagaacttca aacgccgaga
     2041 gaagtgcttc aaatgtggcg tgcccaagtc agaggcagag cagaagctgc ccctcggcac
     2101 gaggctggat cagcagacac tgccactggg tggccgggag ctgagccagg gcctgcttcc
     2161 cctgccgcag ccctaccagg cccagggagt cctggcctcc caagccctgt cacagggctc
     2221 ggagccaagc tcagagaacg ccaatgacac catcattttg cgcaacctga acccacacag
     2281 caccatggat tccatcctgg gggccctggc accctacgcg gtgctgtcct cctccaacgt
     2341 gcgcgtcata aaggacaagc agacccaact gaaccgcggc tttgccttca tccagctctc
     2401 caccatcgtg gaggcagccc agctgctgca gatcctgcag gccctgcacc caccactcac
     2461 tatcgacggc aagaccatca atgttgagtt tgccaagggt tctaagaggg acatggcctc
     2521 caatgaaggc agtcgcatca gtgctgcctc tgtggccagc actgccattg ctgcggccca
     2581 gtgggccatc tcacaggcct cccaaggtgg ggagggtacc tgggccacct ccgaggagcc
     2641 gccggtcgac tacagctact accaacagga tgagggctat ggcaacagcc agggcacaga
     2701 gtcttccctc tatgcccatg gctacctcaa gggcaccaag ggccctggca tcactggaac
     2761 caaaggggat cccactggag caggtcccga ggcctcccta gagcctgggg ccgactctgt
     2821 gtcgatgcag gctttctctc gcgcccagcc tggtgctgct cctggcatct accaacaatc
     2881 agccgaggcg agcagtagcc agggcactgc tgccaacagc cagtcgtata ccatcatgtc
     2941 acccgctgtg ctcaaatctg agctccagag ccctacccat cctagttctg ctctcccacc
     3001 ggctaccagc cccactgccc aggaatccta cagccagtac cctgttcccg acgtctctac
     3061 ctaccagtac gatgagacct ccggctacta ctatgacccc cagaccggcc tctactatga
     3121 ccccaactcc cagtattact acaatgctca gagccagcag tacctgtact gggatgggga
     3181 gaggcggacc tatgttcccg ccctggagca gtcggccgac ggacataagg agacaggggc
     3241 accctcgaag gagggcaaag agaagaagga gaagcacaag accaagacag ctcaacagat
     3301 tgccaaggac atggaacgct gggcccgcag tctcaacaaa caaaaagaaa acttcaaaaa
     3361 tagcttccag cctatcagct ccctgcgaga tgacgagagg cgggagtcag ccactgcaga
     3421 tgctggctat gccatcctcg agaagaaggg agcactagcc gagagacagc acaccagcat
     3481 ggatctcccg aaattggcca gtgacgaccg cccaagccct ccgcgaggac tggtggcagc
     3541 ctacagcggg gagagtgaca gtgaggagga gcaggagcgt gggggccctg agcgggagga
     3601 gaagctcacc gactggcaga agctggcctg tctgctctgc cgacgccagt tccccagcaa
     3661 agaggcgctc atccggcacc agcagctctc agggctccac aagcaaaacc ttgagattca
     3721 ccggcgagcc cacttgtcag aaaacgagct agaagcacta gagaagaatg acatggagca
     3781 aatgaagtac cgggaccgtg cagctgaacg cagagaaaag tatggcatcc ccgagccgcc
     3841 agagcccaag aggaggaagt acggcggcat atccacagcc tctgtagact tcgagcagcc
     3901 tactcgggac gggctgggca gtgacaacat tggcagtcgg atgctgcagg ccatgggctg
     3961 gaaagagggc agcggcctgg gccgcaagaa gcagggcatt gtaacgccta tcgaggccca
     4021 aacacgggtg cggggctccg gcctgggtgc acggggcagc tcctacgggg tcacctcaac
     4081 cgagtcctac aaggagacac tgcacaagac aatggtgacc cgcttcaacg aggcccagtg
     4141 cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     4201 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     4261 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     4321 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     4381 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     4441 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     4501 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     4561 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     4621 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     4681 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     4741 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     4801 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     4861 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     4921 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     4981 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     5041 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     5101 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     5161 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     5221 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     5281 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     5341 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     5401 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     5461 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     5521 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
//
