LOCUS       sequence-169040-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HRP-KDEL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  TITLE     Ting Lab HRP-KDEL plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDisplay-HRP-KDEL
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     155..214
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
      121 cagtgtgctg gaattcggct tggggatatc caccatggag acagacacac tcctgctatg
      181 ggtactgctg ctctgggttc caggttccac tggtgacggg gcccagccgg ccagatctca
      241 gttaacccct acattctacg acaatagctg tcccaacgtg tccaacatcg ttcgcgacac
      301 aatcgtcaac gagctcagat ccgatcccag gatcgctgct tcaatattac gtctgcactt
      361 ccatgactgc ttcgtgaatg gttgcgacgc tagcatatta ctggacaaca ccaccagttt
      421 ccgcactgaa aaggatgcat tcgggaacgc taacagcgcc aggggctttc cagtgatcga
      481 tcgcatgaag gctgccgttg agtcagcatg cccacgaaca gtcagttgtg cagacctgct
      541 gactatagct gcgcaacaga gcgtgactct tgcaggcgga ccgtcctgga gagtgccgct
      601 cggtcgacgt gactccctac aggcattcct agatctggcc aacgccaact tgcctgctcc
      661 attcttcacc ctgccccagc tgaaggatag ctttagaaac gtgggtctga atcgctcgag
      721 tgaccttgtg gctctgtccg gaggacacac atttggaaag aaccagtgta ggttcatcat
      781 ggataggctc tacaatttca gcaacactgg gttacctgac cccacgctga acactacgta
      841 tctccagaca ctgagaggct tgtgcccact gaatggcaac ctcagtgcac tagtggactt
      901 tgatctgcgg accccaacca tcttcgataa caagtactat gtgaatctag aggagcagaa
      961 aggcctgata cagagtgatc aagaactgtt tagcagtcca aacgccactg aca
//
