LOCUS       sequence-169044-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing T-Sapphire fluorescent protein driven 
            by SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, 
            Lamszus K, Fehse B
  TITLE     Optical Barcoding for Single-Clone Tracking to Study Tumor 
            Heterogeneity.
  JOURNAL   Mol Ther. 2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014.
  PUBMED    28109958
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014."
COMMENT     Sequence Label: LeGO-S2
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(56..58,59..61,62..772)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(101..122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(362..381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     709..730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(795..828)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(937..957)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccggatcccg ccaccatggt
       61 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      121 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      241 gaccaccttc tcctacggcg tgatggtgtt cgcccgctac cccgaccaca tgaagcagca
      301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      481 ggagtacaac ttcaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      541 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
      601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      661 gagcatccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      721 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt
      781 cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg
      841 gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta
      901 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
      961 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggc
//
