LOCUS       sequence-169056-       12100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Morales-Martinez A, Dobrzynska A, Askjaer P
  TITLE     Inner nuclear membrane protein LEM-2 is required for correct nuclear
            separation and morphology in C. elegans.
  JOURNAL   J Cell Sci. 2015 Mar 15;128(6):1090-6. doi: 10.1242/jcs.164202. Epub
            2015 Feb 4.
  PUBMED    25653391
REFERENCE   2  (bases 1 to 12100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.164202"; journalName: "J Cell Sci"; date: "2015-03-15- 
            15"; volume: "128"; issue: "6"; pages: "1090-6"
COMMENT     Sequence Label: pBN40
FEATURES             Location/Qualifiers
     source          1..12100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5466..5494)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6242..6262
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(9910..9928)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9949..9965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9949..9965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9962..9984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9973..9989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9997..10003,10004..10021,10022..10027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10042..10063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10180..10197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10351..10939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10431..10450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11110..11901,11902..11970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11733..11752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11971..12075)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccggggccg
      721 cagtctaatg ttttgaggcg tggtccggca ccttgagcaa tgatggccat tgttgtgtga
      781 actccgtagg agatgatcgg aacgacgtca gaatatccct gaaattcaga aaacataaac
      841 acttttgagt aagcaacata cggtatctgt taggttcgtc atgagcaaaa ctgtcattgc
      901 tgcgtttgcc aagattggaa taatcatatt ggagacacga ttcttatcat aatgatatct
      961 ggagaattgt cccattactg tgagtcccat gaacagcacg tagaacataa tattgctgag
     1021 atagagcaag ccaccccact gaaaaatgta tatagttgaa tttgaaacaa ataaagtttg
     1081 cgtaccaccc attttttctc cagcagataa tgatttacaa tgacaataat cgagcaaacg
     1141 gccattccac aatttccggc aatcgcattg gttcgtcggc agacacgtgg agcgacgaca
     1201 cacattgcca aaaataacca gacagcagtt ccgaaatata ttgcaaaatc aactttatat
     1261 cgatagaagc ccatgcgaac atttgcttga agatccggac gtaaccatgc gtagaagtaa
     1321 acactaaata agagtccggc aacgttgata gcgccaatca ttctgaaatt gaagagacag
     1381 gtcagtagtt tgaagctgag cttaaaattt aaaaactatt tagaagaagt accggagaaa
     1441 tgtggcgaaa cacaaaagaa tacgccaaaa agtcggagaa aatgaaaacg aaaaactacc
     1501 gcaaaaaacc attctggtgg atattccttg ccgcaattag ataacaatat agagcaaaag
     1561 aagattcgat ttttttttga tctacctaca aatgacaatt cgaagaaaaa aaaatggaaa
     1621 aaaacaataa cagaaaatgg gtgttaattg aaaacatttt tttaagaaaa cgacggtcct
     1681 tgtccgaatc caccacccat tcctcctgaa gacgccattt cttcttgaag acgtttttga
     1741 agagtttgag aaacaagatt ccatgattcc atgaaacgat ccatgcatcg ctgtagacat
     1801 tgcttttcac cactggccaa tgagcttcct ggtgcagtga tgcatttatt cgtgcacttt
     1861 tcagagatgt cctgcaagca aaaatattat tcaacgttta cattctaaac gccgactaga
     1921 attacatcac tgtcgttttt caatttgata tttgaaaatt ggtgtgagat gacccaaatc
     1981 tatcatttcg gcatccactc catcattttc acaattcttt ggcttaccgt gacaagattc
     2041 tgggcattgg caagggcagc ttgctgcttg acaccactga tcacttgctc ttgctgttct
     2101 ggtgacagct tcttcaacgt ctccacatca agcagctgat ccattttctt ctcctataaa
     2161 tagtctatca tatggcgtgc tcacaaagta acatattttt gaaaaataaa cgtttaggaa
     2221 aagttttcag cataaacatg cattaattca cgcggtatag aaaactgcag ggcgcgtttt
     2281 ctaggcctcg tccacttgtg ttggcgcgaa agttgagatg caattgtatg ggaagatggg
     2341 cgtgtttgtt ctcgtgtttc tttttttttg tgagaagaaa gaaaaatgcg tcagaagagt
     2401 gtcgaaatat ttacgaaaat gttttcccga tttaattact tttcactttc aatcgatgat
     2461 tcttgatgaa ctttgaaggt aacttattta aaaaaaaaaa aaggtgtgta gttttttcta
     2521 atattcattt gaaatatttt tattaaatat tagaacgata aaaaagagat agaaatacaa
     2581 aaaatccaag aaaagttgat gccgttctgt taaggcgcaa gtgtcgattt acgagctggg
     2641 tctcgcatgg atagttgttt taaagggtcg ttatttgaat ttgaagctat ttttcttaaa
     2701 tttttcgtcc cgagtaattc gtgataattc ttttaatctc agataaaagt tttaaattgt
     2761 cgctcatgag aataatcaga ttcttaaaaa aaaaacattt cccgcacatt tttttatcac
     2821 gcttctgctt tcacaaatcc tctctgttct tcataaaacc ttcactgatt taggaaatct
     2881 tcgaagtaat ttttggaagt acacataatt ccggtgcctc ttgttcactt atttcaaggc
     2941 cattttcgtt ctctaattac gccgccttct ttctttgtga ttttcaaatt cagtgtttct
     3001 ttttaacctc ttgcgatttc aattgtcgca cttggtctcc gatctcttaa atagaccaaa
     3061 ggttttatcg aatgagcatg catttttaga gaattgttcg gcgtagcgtt taaaattaaa
     3121 tccgaaatct actttttttt tgtcgtttaa tgtccacgag tatgtttaat tctaattaac
     3181 actagttgcg aaactggtat aatctcgtaa ttcaatacga ccagaaaaat gtctcattcg
     3241 atcggtttct tatgctactt ttcccaccat tcaattcaaa ttcgctcatc ctttcaaggc
     3301 cacaaggaga tgtgtgaaac atgcatctgg tcctgtaatt tatgcaaagc gtcacgtaga
     3361 tttcggtttt cactaacaaa tccttcattt tatatagggc tgtacaaatc ggcacaaaaa
     3421 aaaaaaaaac cggacgcact gcgtccggtt ttggggtagg tttccacggc ggccgacaat
     3481 ttccgatttt cgccactcac tatacttaat cagcaatttt atagtgagtg gcgaaactcg
     3541 gaaattgtcg gccgtcgtgg atagctaccc ccaaccggac gcatgtgcgt ccggttcgga
     3601 cgaccggacg tccggttgta cagccctaat tttatatact caattgttaa tctgcatatt
     3661 accaagggac atgttttgcc caagttcctc tcacatttaa tcgattctct tgttttttca
     3721 tttcaatctt tttagggatc aaatgtgttt tagagatgca ggtgtctaga gaaaattgtt
     3781 ttcctactct ttcatttcgt attcctttca tttcaccaaa cttctctctc gcggctaatc
     3841 tcgccttcat aatagaaatt tagcttctaa ttttcgtgct atactaatat tctaccacat
     3901 ggtcacatca aatgagataa aaactgtcaa cgcgccaaaa tgacactctc ccattgattt
     3961 attgcccttt gtctctgtgc gtcgacgctc tagtgctctt tccagatgtt gtttccttct
     4021 cttctcagcc tccttctcta gaattcattc attctgtgtc aaacaccaaa gaagaacgac
     4081 attgtcttct ctcacacaag actgcttatg atttctcgct tccttcatct ggctctgaat
     4141 atgtctttca atggtacatg tttattgtta atcgccgagt ttcaaaattt acactatcca
     4201 tataaaaaag cggcaaagcc tcaaaatttc agatgtttga ccacttctct aatactctca
     4261 tcatgcctct ccattattcg ttctatttaa ccctgcactg gcatgttgcc aagttgtgta
     4321 tatgtatgtt gtttaccata tgcacacgtc tttcctgctt ctctacttct ctagtcattg
     4381 ggaccgtgag cataaaaaac gaaagaaaaa cttgaaaata gtgcgcgaaa cgggagagat
     4441 cagagatgaa ggggtacgca gcgctcactc gcgcgactaa agagagagca ttggcctggg
     4501 ccattgccct tcgaaacaag atcgagcgtt tttcatcgtt tttgaaccct cgaaactcca
     4561 acatatcacc tctcatgcaa cttttttaca ctaatttgtg taatttgata tcctatctta
     4621 tagtttatca ggtcatttct cttttgtagt agtccgaaag ttttgattga tcgaatttcg
     4681 aaattctccc taaattttcg aaattttgtt actttcaaat tgacgcgaca tggacggtca
     4741 atcaaaacca aacaagtatg cgagaaattc ccttttctct ctttttctct aatcatttca
     4801 gtcccatctg cctagttcta tcggcaattt gtggaagtgc gcagatgatt tttgaaggcg
     4861 aggtgataac gccctgctaa agcattctta ttcacgaggg tttcaaaaaa ctattgaaac
     4921 gaggtaaatc ttagttgagt ataccttgac aattgtagtt ctttgccgag ttgcctaatg
     4981 tttgaattca acgctactta ctgactatgt ttatgctttt gattacgtca ctctcacaaa
     5041 tgttccttat ttctctacta tatttctttc tgttgaaaca tttaaaaaac gaaacaacac
     5101 acatctatgt ggaatactgc catctgccga ttttgaggtc acttggcgcg ccgtaactcg
     5161 caaataacct aacagcgcca gatttttcga caaacacacg ctttccgaag tggtatacac
     5221 aacggccgtg tccgcatgtt tgccacactt cttttgagac agaaaacttt tgttgtataa
     5281 aaattttatc acaagaaata tcaaaatgtt gagtcacgtt cgtgtggcgc ctatgacgtt
     5341 tcctctcttc tcttacatct ctctttgtct atttctgaaa ctaaatccaa tataattaac
     5401 tcttcagaaa gcagcgagaa aagagctcag aaaaaatgag taaaggagaa gaacttttca
     5461 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     5521 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     5581 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
     5641 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
     5701 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     5761 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
     5821 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
     5881 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     5941 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     6001 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
     6061 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     6121 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     6181 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     6241 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     6301 aaaccggtgg cggccgctct agaactagtc caccaaagcc atctgccaag ggagccaaga
     6361 aggccgccaa gaccgtcgtt gccaagccaa aggacggaaa gaagagacgt catgcccgca
     6421 aggaatcgta ctccgtctac atctaccgtg ttctcaagca agttcaccca gacaccggag
     6481 tctcctccaa ggccatgtct atcatgaact ccttcgtcaa cgatgtattc gaacgcatcg
     6541 cttcggaagc ttcccgtctt gctcattaca acaaacgctc aacgatctca tcccgcgaaa
     6601 ttcaaaccgc tgtccgtttg attctcccag gagaacttgc caagcacgcc gtgtctgagg
     6661 gaaccaaggc cgtcaccaag tacacttcca gcaagtaaac tagtggatcc taattttgcc
     6721 gtattttcca tattttgttt tgtatattta tccactcacc ccctctcttt gtcctgtgaa
     6781 tgaacttgtg ccaaaaaagc caaaaataat ttactttttt taaagacata ttaaaacttg
     6841 aaaacttgga acaatacggt gcgaaagcga aacagagaca gaaaaatgaa caaagagatc
     6901 cgtgaaaagt gaacgatgat ttaggcttct tgttgggcgg cgtggatgag agtgatgttg
     6961 tctcctttga gaagaattct gccgatcttg ttgcgtccct tcgtcttcat gttcacctcc
     7021 tcggcttcgt cgaacacgac attcatgaac tcgtcgaatc cgatgatgta gccctcgaga
     7081 cggtgggtga catcctcgta caaccagatt tggacccgag tgcggttttg gagatatctg
     7141 aaatttggga aattgttttc tactgattag tgaaaaaata ttacctgaag atgaggttca
     7201 caggctgaac catcactttg ttgagctttc tcgtcgacat ttttgcggga tgatctgtaa
     7261 aaatcaatat attttttgaa agacacaaat ttttaagata aatatcaaga aaactcgatt
     7321 ttagcagcta tttaagctca aaaactacaa tttatggctt taaaacataa tttttcacat
     7381 tttacataaa tttcaacgaa aaaaaatgcc aaaaaacttc aaatttgagc agaaatcggc
     7441 tattttctcg acaaaaataa tttcgaaaat tcgttcaaaa aagatgagta cgcaagcacc
     7501 gtgacgcaca gttgcggact ttgtaaaccg tacccctgca taaaaaggcg gaattttgaa
     7561 tttaataaaa tgcagtttag aacaaagaaa agcacggaga gaattgtgat aactaattat
     7621 ttgtatttaa ttttaaatac tacataaaac tgaagattgg aacgagttaa agctggtatt
     7681 tgcggtttat agccccaaaa gttaaggctt gttaggcaat tatcgataaa aacaacgaga
     7741 aattcgtgaa atcggcaaaa atttatagaa aacgtttgaa ttaaattaaa tttacgcttt
     7801 tgccgccaac acctatcgag accctagtta taggggcgga gcgtcatggg agcgcgcttg
     7861 caactcgatt tcaaaaagtt tcgctgtttg tacaacattc tcagttgttc ttaatcagtt
     7921 aaataagttt caaaattttc aaaatcataa aacacagtta atgctaaatt aaagtctaaa
     7981 aaatcaggtg ataaggggtt tggacgacca aaaagcgacc ttcgctcgaa aattgataaa
     8041 ctaactacac tttttccgga aaaatgatgg aaaaatcctg attttcttcg ttgaatcaat
     8101 ttttgtttca tttttaacac tttcacatga aatatttcag ttaaaatgct caagctaatt
     8161 gggtttctta taattttggg tctcgcaacg gccgacaagt gctcagattg tttggctcag
     8221 gggaaacagt gagttggaag cttacaattc tgagaattcg tattgcgcag cttatttgac
     8281 gcgcaaaata tgaaatatca cgtagcgaaa actacagtaa ttctttaaat aagactactg
     8341 tagtgcctat gtcgatttac tgcctcaact ttcttaatca agctcgtaaa tcgacaccag
     8401 ctctacagta gtcatagttt tcgctacgag atattttgcg cgtcaaatat ggtgtgcaat
     8461 acgcattttc agaattttat gttctcgtaa tatacctttt ttcagctggt gtgttactgt
     8521 aaatgcatgt ggatttacgc catgtgtcac tacaattaca gtgagttttt ttgaagaaaa
     8581 aaaaattacc catttgatcg ccaattccat ttttcaaaaa tcaataaaaa aaaaaaattt
     8641 cggcgatcag aaacgttaca gaaagcccta aactgcccgg aactaccaga taaagcccac
     8701 gcttacgatg attcatttat gaggaataag gtattgatca cacattctgc tgcctacaat
     8761 atggacccac aaaagtgttt tacgtaggtt caccttctag ataaacctta aaaatcgata
     8821 ctttcagtag taaaatgcct tcgatgaaag tttccaaaac agttaatgtc aattgtgata
     8881 gatatgggcc aagtgagttg ttttattagc ccacggattt ctggcttccc tcataaattg
     8941 aaaaggaaga gattgccgaa ctagaccatt ttggctcggc gcgttgcgtg tcacgtcgcg
     9001 gctcgattgt aaaactaaat gtattggtcc gtgtggagta cacgactttc cctttccggc
     9061 aggcgattgt caatggagcg cgaaaaattc aatgaggaag gccagaaccc cgtgtctgta
     9121 gttttctcaa aaagtggcaa aaactcaata attgccaatt tttgactgcc aataaaaatt
     9181 tcactaaatt tattaaattt attttaagtt ttattgaaat ttttttaaaa tttccccact
     9241 ggcgctaaaa aatccaattt tcagaagcaa tgtgcttcag cttcacggcc atagatactg
     9301 tccaaaaagt gattgcaatg acgtttagag ggacagaagg agcagctcaa ttgtctgaag
     9361 aaattttgga tttcttccag taagttcggg aaatggcaat ttaaagttta attggatttt
     9421 tgtatttttt cttctcacga ctacagtttt gcacattttt atgttataaa aaaccaaaaa
     9481 aaaatttttt tgcctttttt ctcagcttac cacaaaacaa tttcgaccaa aaataaattg
     9541 aaaaaattaa aaaatttttt gcacggtttt cggactcaaa aaaagctgaa tgttcctgga
     9601 actatttgta aaattcaaaa ttcaaaattt tcacgaattc tctaaaaggt tccagcaaat
     9661 tccacaactt tcttgaattt ttcaaaaaag ttcttgagca ttcaacaatt atttttggaa
     9721 ttttccaaag gttacaggac gttccaattt ttcaaaaaaa tttctcgcaa aaaatgtttt
     9781 ctcagaaaat ttgaatttcc ctccacataa aagattgttc tcaaaaaatt tgcaaacaca
     9841 cattttcagc ggaaaaaagg cgttcttcga ctctggaagt gtattcgaat atggtaccag
     9901 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     9961 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10021 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10081 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10141 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
