LOCUS       sequence-169062-       10465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN258
FEATURES             Location/Qualifiers
     source          1..10465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2013..2032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3333..3357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8662..8673
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8822..8846)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10346..10362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10346..10362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10359..10381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgcggcc cattaggtca gacaaaaatt tctgatcttt aaaccttatt gattaaaact
     2341 aatttttgcc cgactttttt gacacatgca ctagacgctt gtactcaaaa tttggaaacc
     2401 ttgctcaaat ccactactca gatatgacag tagcatcgat aattgaacca ttatcctgaa
     2461 atcccctttc ctgtcccggt tcattttcca attttttcaa cggtggaaga aggggcaaat
     2521 tttataccaa aacgtctccg tttatgaccc accccctatg caaaataaac ggctctggga
     2581 gaaatagtac agaaaacgcg gagcctgctt gattccttct ccgaggcgac aaaaaacgca
     2641 tcattcgtat tgtaagatgc atgtgtggct ggcctctttt gaattttact ggttttttat
     2701 atttctacta atatttcgaa aaattttgta agtaaatcat tgattacgtt tacaaaaatt
     2761 tagaaacatt tcaaaatgat tacttctaaa ttaattatat ttacaggtta gttcgagtta
     2821 cttatgacct tccaatccgc catatccgcc aaggacgctc caattaattt tttagaatta
     2881 ttttttaaca catgtattat tttttaacaa taaattattc atgaaatcat caaatgtata
     2941 tgttctacta taatatttag gtcaaactat agaaaagaaa tccttgtttt tggaacaaag
     3001 gagttcagat cctgtgcatt tgatcttgga tccacatctt tggtccatgg gaaagcttgg
     3061 cactgatcaa gaaggattct tcaaatatct aaaattgccg gctgcccgta gaattccatc
     3121 agttttccag agttttagtg aaaatttgca tcccgttaga aacaatctac acaacggtaa
     3181 aaaggaccca ttatttttac cgtaccgttt tcctttatgg ccctctatgc aaaaaattat
     3241 agcgtggagg ggcagtacag aataataaag tgtgagaaaa aaaactgctc tgcgagaaat
     3301 agtacagcaa acgcggagca tgcttgattc tatccacttt gtacaagaaa gctgggttaa
     3361 tacgactcac tagtgggcag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggagc
     3421 cggctgtagc atgcggccgc tacctgcagg acggtatcga taagcttcag taaaagaagt
     3481 agaattttat agtttttttt ctgtttgaaa aattctcccc atcaatgttc tttcaaataa
     3541 atacatcact aatgcaaagt attctataac ctcatatcta aattcttcaa aatcttaaca
     3601 tatcttatca ttgctttaag tcaaacgtaa cattaaaaaa aatgttttgg aaaatgtgtc
     3661 aagtctctca aaattcagtt ttttaaacca ctcctatagt cctatagtcc tatagttacc
     3721 catgaaatcc ttatatatta ctgtaaaatg tttcaaaaac cattggcaaa ttgccagaac
     3781 tgaaaatttc cggcaaattg gggaaccggc aaattgccaa tttgctgaat ttgccggaaa
     3841 aacggtaatt gccgaaagtt tttgacacga aaatggcaaa ttgtggtttt aaaatttttt
     3901 tttttggaaa tttcagaatt tcaattttaa tcggcaaaac tgtaggcatc ctaagaatgt
     3961 tcctacatct attttgaaaa gtaagcgaat tctatgaaaa tgtctaaaga aaatggggaa
     4021 acaatttcaa aaaggcacag tttcaagtgt ttccgaatta tactaaatcc ctctaaaaac
     4081 ttccggcaaa ttgatatccg taaaagagca aatccgcatt tttgccgaaa attaaaattt
     4141 ccgacaaatc ggcaaaccgg caatttggcg aaatttgccg gaaggcaatt gccgcccacc
     4201 cctgttccag aggttcaaac tggtagcaaa gctcaaaatt tctcaaattc tccaattttt
     4261 ttttgaattt tggcagtgta ccaaaatgac attcagtcat attggtttat tatagattta
     4321 tttagataaa atcctaaatg attctacctt taaagatgcc cactttaaaa gtaatgactc
     4381 aaacttcaaa ttgctctaag attctattga attaccatct tttcctctca ttttctctca
     4441 ctgtctattt catcacaaat tcatccctct ctccctctct ttctctctcc ctctctctct
     4501 ttctctttgc tcatcatctg tcattttgtc cgttcctctc tctgcgccct cagcgttccc
     4561 cacactctct cgcttctctt ttcctagacg tcttcttttt tcatcttctt cagccctttt
     4621 tcgccatttt ccatctctgt caatcattac ggacgacccc cattaatttt tggatgtgct
     4681 ctgcgagcaa gagcacacgg aactcattta ttttttgttg gaatttcttt ttcattttag
     4741 ttagttaaaa gctacaacag catatggtac caaaaatgcc acaattcgac attctctgta
     4801 agacaccacc aaaagtcctc gtccgtcaat tcgtcgagcg tttcgagcgt ccatccggag
     4861 agaagatcgc cctctgcgcc gccgagctca cctacctctg ctggatgatc acccacaacg
     4921 gaaccgccat caagcgtgcc accttcatgt cctacaacac catcatctcc aactccctct
     4981 ccttcgacat cgtcaacaag tccctccaat tcaagtacaa gacccaaaag gccaccatcc
     5041 tcgaggcctc cctcaaggta agtttaaaca tatatatact aactaaccct gattatttaa
     5101 attttcagaa gctcatccca gcctgggagt tcaccatcat cccatactac ggacaaaagc
     5161 accaatccga catcaccgac atcgtctcct ccctccaact ccaattcgag tcctccgagg
     5221 aggccgacaa gggaaactcc cactccaaga agatgctcaa ggccctcctc tccgagggag
     5281 agtccatctg ggagatcacc gagaagatcc tcaactcctt cgagtacacc tcccgtttca
     5341 ccaagaccaa gaccctctac caattcctct tcctcgccac cttcatcaac tgcggacgtt
     5401 tctccgacat caagaacgtc gacccaaagt ccttcaagct cgtccaaaac aagtacctcg
     5461 gagtcatcat ccaatgcctc gtcaccgaga ccaagacctc cgtctcccgt cacatctact
     5521 tcttctccgc ccgtggacgt atcgacccac tcgtctacct cgacgagttc ctccgtaact
     5581 ccgagccagt cctcaagcgt gtcaaccgta ccggaaactc ctcctccaac aagcaagagt
     5641 accaactcct caaggacaac ctcgtccgtt cctacaacaa ggccctcaag aagaacgccc
     5701 catactccat cttcgccatc aagaacggac caaagtccca catcggacgt cacctcatga
     5761 cctccttcct ctccatgaag gtaagtttaa acatgatttt actaactaac taatctgatt
     5821 taaattttca gggactcacc gagctcacca acgtcgtcgg aaactggtcc gacaagcgtg
     5881 cctccgccgt cgcccgtacc acctacaccc accaaatcac cgccatccca gaccactact
     5941 tcgccctcgt ctcccgttac tacgcctacg acccaatctc caaggagatg atcgccctca
     6001 aggacgagac caacccaatc gaggagtggc aacacatcga gcaactcaag ggatccgccg
     6061 agggatccat ccgttaccca gcctggaacg gaatcatctc ccaagaggtc ctcgactacc
     6121 tctcctccta catcaaccgt cgtatctaac gcgtatgtat ctgcatatcc ttcaaatgtt
     6181 gtccatattg tatcagtaaa ttataatgcc ccctttttat atattctcct tatcatgtaa
     6241 atgttctatt gattttttgc tggtgtaaaa cgtttttata gtaaatcctc tccacagtga
     6301 atttgttaaa ttgtatcaaa acaagacatt gattaaattt gttcgagact tgtggattgt
     6361 aaaatatttt gcagaaaatg gcaatattca tttagaaaca ttacttccat aataggaaaa
     6421 actatttatg agttttcatg tgcctatgca agttcccact ctgtgtgaat ttcatcatac
     6481 aaattgagca aacataaggt ttatgtccag tatgggttct cagatgtttt gtaagagttg
     6541 agctgtcggt gaatgccttc tcacattgag cacatggata cggtctctct ccagtgtgtg
     6601 ttctgaaata atgaaaaata gtcttgcaat atgtttctac tattcgagaa aaaatgtaat
     6661 tttttttctt atggtaaccc ggctttcccc gggcctagtt ctagacattc tctaatgaaa
     6721 aaatctttca gttgaaattg aaaatgagtt aaagttggag tttttattga aaacagattt
     6781 ccgtgtgatt agtgttttta gcgagtgtga caggacagcg aaaaaatata gaaacaaggg
     6841 gggaactgaa aagcttagga atgcattgaa catgagaagg ggaaggggaa ggaacaaact
     6901 agacaggaat tattggaatt taatcacatt tggagttttt tttctattcg acagaataat
     6961 tatccagaac atttttgtat taaatattta tgcatcatat gagtagtcgg ctttgttgtg
     7021 catgacgagt ttgttatcga cgaaatagaa gctgtcagaa cgagtctcgt ttggattgtt
     7081 gatcatgtcg tccactgaaa aagagattag tcttttgaat tgtacttttt agaataatga
     7141 ctcactgagt tgttgagaga gttgagggaa ctcatagata tgttcacagt tgtttcgtga
     7201 attcggaata cagaatccga attcaaagtc aaaacacttc agaaggcgat ctttgaagaa
     7261 gtgacgttcg atcattcgga aatgatggat tgggatgtct cctactttga attccacagt
     7321 tgctccgacc gttttgagtt tcaaaaagtt tggagcaaat ctataacgca cgtatcttgc
     7381 cgactcctgt ggcgactcat cattctcctg atcgttctcc ggtttggcga tctcgaaaag
     7441 cacttgctca gtgtccagat cacggatttg gaacttggtg aactcgatgt tatagatgtt
     7501 cgcagatggg gagcataaga atcctaaatt tatgttttaa actgaaatcc aaagggagca
     7561 agataccttg agtgattccc ggaagtgcta aaacgtcgtt cggagtgatt tgagctttct
     7621 tcgcaagctc cgattccgtt gtgattcctt gttcggtgct tggtggtggc cgtggcatct
     7681 ggaaatatgg aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg atatcaagag
     7741 ttagttgagc ggtttctcta gttttctgag tctcacctgc gacgggaagg tcgccgagcc
     7801 gggtggaatc gatcgttgtt gctcggcttt catatcggtt tggttggaag cggctgaaaa
     7861 cggaaagaag tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta attagagggt
     7921 gccaaataac cagctatatt ttgttttttt ttgaaaacat ttttaaaaag aaaaatacga
     7981 taatgatatc agatggattt ccggaaaact ggtatgaaaa atttcaacct ttttgagtac
     8041 atgtaatcaa aatacacttt gtaaattatc atttttattg aaactccacc atttttctat
     8101 ttataacgct aataatttga aaaagaaacc tattgcgaac cgcggggtga atcccaaaaa
     8161 cgaatgcgtt ttggtggagt gattgattcg aatcgaagaa gaaaaagaag aagacgtgga
     8221 atagagagct cactcttaac cgagcagcac acaccgacag aaaaaaaaat gaaatgaatg
     8281 agggtcttct tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag aggaagattg
     8341 agaagggaaa aaggaaggag aaaagaagca gaagaagacg tcagagagga gaggaacgag
     8401 cggaaaagca gcgggcgcaa gtcatagaag tagcagagct ggggagaaga agacactatc
     8461 caagaaagga atgacgagag agtatgcaaa ggggtatagg gtgcagacag aataggaaca
     8521 gaataacaga tgatgagcca agaagagttg aaaagggcga tgaatttgtc atgtaactta
     8581 atttgggtca atttgagcat gatgaattga aatcatccct tgttgggagt taataaccgg
     8641 tttgttatca gaaaccctgt aatagaaggg cgccctaact ttgagccaat tcatcccggt
     8701 ttctgtcaaa tatatcaaaa agtggtcaac tgacaaattg tttttgatat tataataaac
     8761 attttatccg ttaacaattt tcgaatactt tttacaagga cttggataaa ttggctcaaa
     8821 gagcctgctt ttttgtacaa acttgaattc aaaaacgcag aactcgtact gttggatgcc
     8881 tgtgtagcgg tcctctattg atttttgcta ttttttgata attttactgt aaattatgag
     8941 ttattccaat taacattcct agctaaatgt aagttagcga ccaatttttt agcaacccca
     9001 ttttatgact tttcagaata tcgcctactg ggtcaacgac acttgtttgg aactaagggt
     9061 cttcccaatt ttttttaaat cttcataggc ttgacagaat cagggttctt caagcagctt
     9121 tgagagggaa tcaattgcaa catttgatgt taaatttata ttttttatag ttctctggtg
     9181 aaatggggac caccgcaaag ccccttttat gtttttaact tgaataaaat cttgtattaa
     9241 attgtgattt atagatctgt atgggtaatt tgaaggaaaa gaaagggagc tcccaaaagt
     9301 gccttacaga tccaggccca ataaatgtaa actattgggc agtagtatgg caaaaatcgt
     9361 gcggaatttg aaaaacctag aattaaaata aatattcaac acaaatgttc gagatatttt
     9421 aagttcggag tgtatatcta gaacatattg tacaagtagg tctacgggat cgtctacggg
     9481 aaaatttgat ttcctcgaag atgtatttaa tgaattctct aatgttgcag gtctttgtat
     9541 ttgacagaat gttggttttt gaaacttatt cgccataatt gcttggctac tacatgtctc
     9601 atgccattga cactatcctg tactcgaaat ttgttaagta actacccacc gtccacttat
     9661 gacgagaggg tcaattaagg tcactccaca aaagtaaatt tgaaaacaca tgagaaaaag
     9721 tgatctgaat tcccttgaag tggctaaaac aaagcagatg agggatacga cgtatgcgac
     9781 tactgagaga taaggtcgaa acttgataag gatgctgctt acctgcttag caagtttctt
     9841 cgcacaggag aaagtgacaa gctaaactat caaattcatc taaaacctca aaaaattatt
     9901 ttggatgaaa taatgtttta ttttgagcaa cgcataaggc aaagaataaa ttaaaggcga
     9961 tccgcttagc ggcgagttcc gtcctcgaag aagattcctc catcgatggc gcagtacttt
    10021 ggacagattc cacgctctcc tggggttcct ggtggtccga ctggtcctgg ctgtcctggg
    10081 tttccgtcgg ctcctggctt gccgtctggt cctggtggtc cctttggtcc aggttgtcct
    10141 gaaagatgtg gtgattagat atggattgaa aaactatgaa atgattacct ggggttccgt
    10201 cgtttcctgg ttgtccggat ggtcctggtg gtccgactgg tcctggctct ccggtggatc
    10261 ctggctctcc cttttggact ggctcgcaga cagctggaag tccctccaac tttctataca
    10321 aagttgatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    10381 tggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10441 gaaagaacat gtgagcaaaa ggcca
//
