LOCUS       sequence-169065-        9992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN278
FEATURES             Location/Qualifiers
     source          1..9992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2013..2032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3333..3357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8189..8200
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8349..8373)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9873..9889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9873..9889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9886..9908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgcggcc cattaggtca gacaaaaatt tctgatcttt aaaccttatt gattaaaact
     2341 aatttttgcc cgactttttt gacacatgca ctagacgctt gtactcaaaa tttggaaacc
     2401 ttgctcaaat ccactactca gatatgacag tagcatcgat aattgaacca ttatcctgaa
     2461 atcccctttc ctgtcccggt tcattttcca attttttcaa cggtggaaga aggggcaaat
     2521 tttataccaa aacgtctccg tttatgaccc accccctatg caaaataaac ggctctggga
     2581 gaaatagtac agaaaacgcg gagcctgctt gattccttct ccgaggcgac aaaaaacgca
     2641 tcattcgtat tgtaagatgc atgtgtggct ggcctctttt gaattttact ggttttttat
     2701 atttctacta atatttcgaa aaattttgta agtaaatcat tgattacgtt tacaaaaatt
     2761 tagaaacatt tcaaaatgat tacttctaaa ttaattatat ttacaggtta gttcgagtta
     2821 cttatgacct tccaatccgc catatccgcc aaggacgctc caattaattt tttagaatta
     2881 ttttttaaca catgtattat tttttaacaa taaattattc atgaaatcat caaatgtata
     2941 tgttctacta taatatttag gtcaaactat agaaaagaaa tccttgtttt tggaacaaag
     3001 gagttcagat cctgtgcatt tgatcttgga tccacatctt tggtccatgg gaaagcttgg
     3061 cactgatcaa gaaggattct tcaaatatct aaaattgccg gctgcccgta gaattccatc
     3121 agttttccag agttttagtg aaaatttgca tcccgttaga aacaatctac acaacggtaa
     3181 aaaggaccca ttatttttac cgtaccgttt tcctttatgg ccctctatgc aaaaaattat
     3241 agcgtggagg ggcagtacag aataataaag tgtgagaaaa aaaactgctc tgcgagaaat
     3301 agtacagcaa acgcggagca tgcttgattc tatccacttt gtacaagaaa gctgggttaa
     3361 tacgactcac tagtgggcag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggagc
     3421 cggctgtagc atgcggccgc gtagtaatct agaaatgtaa acctgtcatt tctgtgtatt
     3481 tcagcaccgc gaagagcacc ataaacagct acaacaagag cacgaacgtc ggctccaaca
     3541 gaaaaagctg caaaacgctg gtactcacca catgctctga taattgccat ttccttcgat
     3601 ttctgacttt caattgtgat atgataacct caaaatctcc attcaaaatg aaaactctta
     3661 aatttaagag ctttcaacac cccgcacttt cccacctcat cgtttttccc ttctatctta
     3721 tcaatttttt gtattttgtg tcagtttgct atatcctcat cacctttttt accgtttaat
     3781 ctctctatag tattgttcgg gtgtttcaag atgaaacgtt ttgtgtgtag catttttgaa
     3841 acacagcgcg aaaaatgcag aaactatcat ccacaatgca ttaagtgcac tcattgtgat
     3901 gactcctaac cccctcgccc caccagttgt ctcttcacaa attcatggca caattcaaaa
     3961 atggcttttt gtgttggatc ttgatctcgt ctctttcctt cttctttcgt ttctctcgga
     4021 ccactcatta ttcgaatgcc atgcatttgt tgatgatgcg cagcgatggt cgcacacact
     4081 acaacagata tgatggtggc tgtgcgtggt gcagagctca tgaataagtg atggggcacc
     4141 gagatgtgac tcctcccatt attcgtcttg agtcgcatct tttgtcttgg tccgttgtca
     4201 gtcgagcctc aaggcagccg ttccgccagt gatcgcctgc taagaagttg taggtgtaga
     4261 ggaagaaacg tcgggtccaa atttcaagca acggtaccaa aaatgccaca attcgacatt
     4321 ctctgtaaga caccaccaaa agtcctcgtc cgtcaattcg tcgagcgttt cgagcgtcca
     4381 tccggagaga agatcgccct ctgcgccgcc gagctcacct acctctgctg gatgatcacc
     4441 cacaacggaa ccgccatcaa gcgtgccacc ttcatgtcct acaacaccat catctccaac
     4501 tccctctcct tcgacatcgt caacaagtcc ctccaattca agtacaagac ccaaaaggcc
     4561 accatcctcg aggcctccct caaggtaagt ttaaacatat atatactaac taaccctgat
     4621 tatttaaatt ttcagaagct catcccagcc tgggagttca ccatcatccc atactacgga
     4681 caaaagcacc aatccgacat caccgacatc gtctcctccc tccaactcca attcgagtcc
     4741 tccgaggagg ccgacaaggg aaactcccac tccaagaaga tgctcaaggc cctcctctcc
     4801 gagggagagt ccatctggga gatcaccgag aagatcctca actccttcga gtacacctcc
     4861 cgtttcacca agaccaagac cctctaccaa ttcctcttcc tcgccacctt catcaactgc
     4921 ggacgtttct ccgacatcaa gaacgtcgac ccaaagtcct tcaagctcgt ccaaaacaag
     4981 tacctcggag tcatcatcca atgcctcgtc accgagacca agacctccgt ctcccgtcac
     5041 atctacttct tctccgcccg tggacgtatc gacccactcg tctacctcga cgagttcctc
     5101 cgtaactccg agccagtcct caagcgtgtc aaccgtaccg gaaactcctc ctccaacaag
     5161 caagagtacc aactcctcaa ggacaacctc gtccgttcct acaacaaggc cctcaagaag
     5221 aacgccccat actccatctt cgccatcaag aacggaccaa agtcccacat cggacgtcac
     5281 ctcatgacct ccttcctctc catgaaggta agtttaaaca tgattttact aactaactaa
     5341 tctgatttaa attttcaggg actcaccgag ctcaccaacg tcgtcggaaa ctggtccgac
     5401 aagcgtgcct ccgccgtcgc ccgtaccacc tacacccacc aaatcaccgc catcccagac
     5461 cactacttcg ccctcgtctc ccgttactac gcctacgacc caatctccaa ggagatgatc
     5521 gccctcaagg acgagaccaa cccaatcgag gagtggcaac acatcgagca actcaaggga
     5581 tccgccgagg gatccatccg ttacccagcc tggaacggaa tcatctccca agaggtcctc
     5641 gactacctct cctcctacat caaccgtcgt atctaacgcg tatgtatctg catatccttc
     5701 aaatgttgtc catattgtat cagtaaatta taatgccccc tttttatata ttctccttat
     5761 catgtaaatg ttctattgat tttttgctgg tgtaaaacgt ttttatagta aatcctctcc
     5821 acagtgaatt tgttaaattg tatcaaaaca agacattgat taaatttgtt cgagacttgt
     5881 ggattgtaaa atattttgca gaaaatggca atattcattt agaaacatta cttccataat
     5941 aggaaaaact atttatgagt tttcatgtgc ctatgcaagt tcccactctg tgtgaatttc
     6001 atcatacaaa ttgagcaaac ataaggttta tgtccagtat gggttctcag atgttttgta
     6061 agagttgagc tgtcggtgaa tgccttctca cattgagcac atggatacgg tctctctcca
     6121 gtgtgtgttc tgaaataatg aaaaatagtc ttgcaatatg tttctactat tcgagaaaaa
     6181 atgtaatttt ttttcttatg gtaacccggc tttccccggg cctagttcta gacattctct
     6241 aatgaaaaaa tctttcagtt gaaattgaaa atgagttaaa gttggagttt ttattgaaaa
     6301 cagatttccg tgtgattagt gtttttagcg agtgtgacag gacagcgaaa aaatatagaa
     6361 acaagggggg aactgaaaag cttaggaatg cattgaacat gagaagggga aggggaagga
     6421 acaaactaga caggaattat tggaatttaa tcacatttgg agtttttttt ctattcgaca
     6481 gaataattat ccagaacatt tttgtattaa atatttatgc atcatatgag tagtcggctt
     6541 tgttgtgcat gacgagtttg ttatcgacga aatagaagct gtcagaacga gtctcgtttg
     6601 gattgttgat catgtcgtcc actgaaaaag agattagtct tttgaattgt actttttaga
     6661 ataatgactc actgagttgt tgagagagtt gagggaactc atagatatgt tcacagttgt
     6721 ttcgtgaatt cggaatacag aatccgaatt caaagtcaaa acacttcaga aggcgatctt
     6781 tgaagaagtg acgttcgatc attcggaaat gatggattgg gatgtctcct actttgaatt
     6841 ccacagttgc tccgaccgtt ttgagtttca aaaagtttgg agcaaatcta taacgcacgt
     6901 atcttgccga ctcctgtggc gactcatcat tctcctgatc gttctccggt ttggcgatct
     6961 cgaaaagcac ttgctcagtg tccagatcac ggatttggaa cttggtgaac tcgatgttat
     7021 agatgttcgc agatggggag cataagaatc ctaaatttat gttttaaact gaaatccaaa
     7081 gggagcaaga taccttgagt gattcccgga agtgctaaaa cgtcgttcgg agtgatttga
     7141 gctttcttcg caagctccga ttccgttgtg attccttgtt cggtgcttgg tggtggccgt
     7201 ggcatctgga aatatggaaa agttcaacaa aaagaaaaga gaaaagaatg aaatcggata
     7261 tcaagagtta gttgagcggt ttctctagtt ttctgagtct cacctgcgac gggaaggtcg
     7321 ccgagccggg tggaatcgat cgttgttgct cggctttcat atcggtttgg ttggaagcgg
     7381 ctgaaaacgg aaagaagtgg aagaaggaaa agagtgtggt gtgacaggaa aatggtaatt
     7441 agagggtgcc aaataaccag ctatattttg tttttttttg aaaacatttt taaaaagaaa
     7501 aatacgataa tgatatcaga tggatttccg gaaaactggt atgaaaaatt tcaacctttt
     7561 tgagtacatg taatcaaaat acactttgta aattatcatt tttattgaaa ctccaccatt
     7621 tttctattta taacgctaat aatttgaaaa agaaacctat tgcgaaccgc ggggtgaatc
     7681 ccaaaaacga atgcgttttg gtggagtgat tgattcgaat cgaagaagaa aaagaagaag
     7741 acgtggaata gagagctcac tcttaaccga gcagcacaca ccgacagaaa aaaaaatgaa
     7801 atgaatgagg gtcttcttct tcttcttctt cgaatgattg acagaaatgg gaaaaagagg
     7861 aagattgaga agggaaaaag gaaggagaaa agaagcagaa gaagacgtca gagaggagag
     7921 gaacgagcgg aaaagcagcg ggcgcaagtc atagaagtag cagagctggg gagaagaaga
     7981 cactatccaa gaaaggaatg acgagagagt atgcaaaggg gtatagggtg cagacagaat
     8041 aggaacagaa taacagatga tgagccaaga agagttgaaa agggcgatga atttgtcatg
     8101 taacttaatt tgggtcaatt tgagcatgat gaattgaaat catcccttgt tgggagttaa
     8161 taaccggttt gttatcagaa accctgtaat agaagggcgc cctaactttg agccaattca
     8221 tcccggtttc tgtcaaatat atcaaaaagt ggtcaactga caaattgttt ttgatattat
     8281 aataaacatt ttatccgtta acaattttcg aatacttttt acaaggactt ggataaattg
     8341 gctcaaagag cctgcttttt tgtacaaact tgaattcaaa aacgcagaac tcgtactgtt
     8401 ggatgcctgt gtagcggtcc tctattgatt tttgctattt tttgataatt ttactgtaaa
     8461 ttatgagtta ttccaattaa cattcctagc taaatgtaag ttagcgacca attttttagc
     8521 aaccccattt tatgactttt cagaatatcg cctactgggt caacgacact tgtttggaac
     8581 taagggtctt cccaattttt tttaaatctt cataggcttg acagaatcag ggttcttcaa
     8641 gcagctttga gagggaatca attgcaacat ttgatgttaa atttatattt tttatagttc
     8701 tctggtgaaa tggggaccac cgcaaagccc cttttatgtt tttaacttga ataaaatctt
     8761 gtattaaatt gtgatttata gatctgtatg ggtaatttga aggaaaagaa agggagctcc
     8821 caaaagtgcc ttacagatcc aggcccaata aatgtaaact attgggcagt agtatggcaa
     8881 aaatcgtgcg gaatttgaaa aacctagaat taaaataaat attcaacaca aatgttcgag
     8941 atattttaag ttcggagtgt atatctagaa catattgtac aagtaggtct acgggatcgt
     9001 ctacgggaaa atttgatttc ctcgaagatg tatttaatga attctctaat gttgcaggtc
     9061 tttgtatttg acagaatgtt ggtttttgaa acttattcgc cataattgct tggctactac
     9121 atgtctcatg ccattgacac tatcctgtac tcgaaatttg ttaagtaact acccaccgtc
     9181 cacttatgac gagagggtca attaaggtca ctccacaaaa gtaaatttga aaacacatga
     9241 gaaaaagtga tctgaattcc cttgaagtgg ctaaaacaaa gcagatgagg gatacgacgt
     9301 atgcgactac tgagagataa ggtcgaaact tgataaggat gctgcttacc tgcttagcaa
     9361 gtttcttcgc acaggagaaa gtgacaagct aaactatcaa attcatctaa aacctcaaaa
     9421 aattattttg gatgaaataa tgttttattt tgagcaacgc ataaggcaaa gaataaatta
     9481 aaggcgatcc gcttagcggc gagttccgtc ctcgaagaag attcctccat cgatggcgca
     9541 gtactttgga cagattccac gctctcctgg ggttcctggt ggtccgactg gtcctggctg
     9601 tcctgggttt ccgtcggctc ctggcttgcc gtctggtcct ggtggtccct ttggtccagg
     9661 ttgtcctgaa agatgtggtg attagatatg gattgaaaaa ctatgaaatg attacctggg
     9721 gttccgtcgt ttcctggttg tccggatggt cctggtggtc cgactggtcc tggctctccg
     9781 gtggatcctg gctctccctt ttggactggc tcgcagacag ctggaagtcc ctccaacttt
     9841 ctatacaaag ttgatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9901 tatccgctgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9961 aacgcaggaa agaacatgtg agcaaaaggc ca
//
