LOCUS       sequence-169072-        9517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN280
FEATURES             Location/Qualifiers
     source          1..9517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2013..2032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3333..3357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7714..7725
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7874..7898)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9398..9414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9398..9414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9411..9433)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgcggcc cattaggtca gacaaaaatt tctgatcttt aaaccttatt gattaaaact
     2341 aatttttgcc cgactttttt gacacatgca ctagacgctt gtactcaaaa tttggaaacc
     2401 ttgctcaaat ccactactca gatatgacag tagcatcgat aattgaacca ttatcctgaa
     2461 atcccctttc ctgtcccggt tcattttcca attttttcaa cggtggaaga aggggcaaat
     2521 tttataccaa aacgtctccg tttatgaccc accccctatg caaaataaac ggctctggga
     2581 gaaatagtac agaaaacgcg gagcctgctt gattccttct ccgaggcgac aaaaaacgca
     2641 tcattcgtat tgtaagatgc atgtgtggct ggcctctttt gaattttact ggttttttat
     2701 atttctacta atatttcgaa aaattttgta agtaaatcat tgattacgtt tacaaaaatt
     2761 tagaaacatt tcaaaatgat tacttctaaa ttaattatat ttacaggtta gttcgagtta
     2821 cttatgacct tccaatccgc catatccgcc aaggacgctc caattaattt tttagaatta
     2881 ttttttaaca catgtattat tttttaacaa taaattattc atgaaatcat caaatgtata
     2941 tgttctacta taatatttag gtcaaactat agaaaagaaa tccttgtttt tggaacaaag
     3001 gagttcagat cctgtgcatt tgatcttgga tccacatctt tggtccatgg gaaagcttgg
     3061 cactgatcaa gaaggattct tcaaatatct aaaattgccg gctgcccgta gaattccatc
     3121 agttttccag agttttagtg aaaatttgca tcccgttaga aacaatctac acaacggtaa
     3181 aaaggaccca ttatttttac cgtaccgttt tcctttatgg ccctctatgc aaaaaattat
     3241 agcgtggagg ggcagtacag aataataaag tgtgagaaaa aaaactgctc tgcgagaaat
     3301 agtacagcaa acgcggagca tgcttgattc tatccacttt gtacaagaaa gctgggttaa
     3361 tacgactcac tagtgggcag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggagc
     3421 cggctgtagc atgcggccgc gtagtaagct ccgatgcgtt cccgttcatt attcttcttc
     3481 aataaattcg aaatctgaca tcattctcat cttttcccat catcacaagc cgtgggctca
     3541 tttattctcc cacggaaacc atgacagcaa aaataaatag agtggcgcct tattcgactc
     3601 atttcgtttt tttttctccg gatattagat tgtgtggcag gcggctccat tgtatattac
     3661 gtgccgaatt ccagaagcac cacgccatcg gatatctaaa agaggaggtg tctttgtttg
     3721 cgcataataa aacaatcaat caacacagca aagacccctc tcaacctcat ttcatgattt
     3781 tctttggttt ttaggtagca ttgctctctt caatcatggt accaaaaatg ccacaattcg
     3841 acattctctg taagacacca ccaaaagtcc tcgtccgtca attcgtcgag cgtttcgagc
     3901 gtccatccgg agagaagatc gccctctgcg ccgccgagct cacctacctc tgctggatga
     3961 tcacccacaa cggaaccgcc atcaagcgtg ccaccttcat gtcctacaac accatcatct
     4021 ccaactccct ctccttcgac atcgtcaaca agtccctcca attcaagtac aagacccaaa
     4081 aggccaccat cctcgaggcc tccctcaagg taagtttaaa catatatata ctaactaacc
     4141 ctgattattt aaattttcag aagctcatcc cagcctggga gttcaccatc atcccatact
     4201 acggacaaaa gcaccaatcc gacatcaccg acatcgtctc ctccctccaa ctccaattcg
     4261 agtcctccga ggaggccgac aagggaaact cccactccaa gaagatgctc aaggccctcc
     4321 tctccgaggg agagtccatc tgggagatca ccgagaagat cctcaactcc ttcgagtaca
     4381 cctcccgttt caccaagacc aagaccctct accaattcct cttcctcgcc accttcatca
     4441 actgcggacg tttctccgac atcaagaacg tcgacccaaa gtccttcaag ctcgtccaaa
     4501 acaagtacct cggagtcatc atccaatgcc tcgtcaccga gaccaagacc tccgtctccc
     4561 gtcacatcta cttcttctcc gcccgtggac gtatcgaccc actcgtctac ctcgacgagt
     4621 tcctccgtaa ctccgagcca gtcctcaagc gtgtcaaccg taccggaaac tcctcctcca
     4681 acaagcaaga gtaccaactc ctcaaggaca acctcgtccg ttcctacaac aaggccctca
     4741 agaagaacgc cccatactcc atcttcgcca tcaagaacgg accaaagtcc cacatcggac
     4801 gtcacctcat gacctccttc ctctccatga aggtaagttt aaacatgatt ttactaacta
     4861 actaatctga tttaaatttt cagggactca ccgagctcac caacgtcgtc ggaaactggt
     4921 ccgacaagcg tgcctccgcc gtcgcccgta ccacctacac ccaccaaatc accgccatcc
     4981 cagaccacta cttcgccctc gtctcccgtt actacgccta cgacccaatc tccaaggaga
     5041 tgatcgccct caaggacgag accaacccaa tcgaggagtg gcaacacatc gagcaactca
     5101 agggatccgc cgagggatcc atccgttacc cagcctggaa cggaatcatc tcccaagagg
     5161 tcctcgacta cctctcctcc tacatcaacc gtcgtatcta acgcgtatgt atctgcatat
     5221 ccttcaaatg ttgtccatat tgtatcagta aattataatg cccccttttt atatattctc
     5281 cttatcatgt aaatgttcta ttgatttttt gctggtgtaa aacgttttta tagtaaatcc
     5341 tctccacagt gaatttgtta aattgtatca aaacaagaca ttgattaaat ttgttcgaga
     5401 cttgtggatt gtaaaatatt ttgcagaaaa tggcaatatt catttagaaa cattacttcc
     5461 ataataggaa aaactattta tgagttttca tgtgcctatg caagttccca ctctgtgtga
     5521 atttcatcat acaaattgag caaacataag gtttatgtcc agtatgggtt ctcagatgtt
     5581 ttgtaagagt tgagctgtcg gtgaatgcct tctcacattg agcacatgga tacggtctct
     5641 ctccagtgtg tgttctgaaa taatgaaaaa tagtcttgca atatgtttct actattcgag
     5701 aaaaaatgta attttttttc ttatggtaac ccggctttcc ccgggcctag ttctagacat
     5761 tctctaatga aaaaatcttt cagttgaaat tgaaaatgag ttaaagttgg agtttttatt
     5821 gaaaacagat ttccgtgtga ttagtgtttt tagcgagtgt gacaggacag cgaaaaaata
     5881 tagaaacaag gggggaactg aaaagcttag gaatgcattg aacatgagaa ggggaagggg
     5941 aaggaacaaa ctagacagga attattggaa tttaatcaca tttggagttt tttttctatt
     6001 cgacagaata attatccaga acatttttgt attaaatatt tatgcatcat atgagtagtc
     6061 ggctttgttg tgcatgacga gtttgttatc gacgaaatag aagctgtcag aacgagtctc
     6121 gtttggattg ttgatcatgt cgtccactga aaaagagatt agtcttttga attgtacttt
     6181 ttagaataat gactcactga gttgttgaga gagttgaggg aactcataga tatgttcaca
     6241 gttgtttcgt gaattcggaa tacagaatcc gaattcaaag tcaaaacact tcagaaggcg
     6301 atctttgaag aagtgacgtt cgatcattcg gaaatgatgg attgggatgt ctcctacttt
     6361 gaattccaca gttgctccga ccgttttgag tttcaaaaag tttggagcaa atctataacg
     6421 cacgtatctt gccgactcct gtggcgactc atcattctcc tgatcgttct ccggtttggc
     6481 gatctcgaaa agcacttgct cagtgtccag atcacggatt tggaacttgg tgaactcgat
     6541 gttatagatg ttcgcagatg gggagcataa gaatcctaaa tttatgtttt aaactgaaat
     6601 ccaaagggag caagatacct tgagtgattc ccggaagtgc taaaacgtcg ttcggagtga
     6661 tttgagcttt cttcgcaagc tccgattccg ttgtgattcc ttgttcggtg cttggtggtg
     6721 gccgtggcat ctggaaatat ggaaaagttc aacaaaaaga aaagagaaaa gaatgaaatc
     6781 ggatatcaag agttagttga gcggtttctc tagttttctg agtctcacct gcgacgggaa
     6841 ggtcgccgag ccgggtggaa tcgatcgttg ttgctcggct ttcatatcgg tttggttgga
     6901 agcggctgaa aacggaaaga agtggaagaa ggaaaagagt gtggtgtgac aggaaaatgg
     6961 taattagagg gtgccaaata accagctata ttttgttttt ttttgaaaac atttttaaaa
     7021 agaaaaatac gataatgata tcagatggat ttccggaaaa ctggtatgaa aaatttcaac
     7081 ctttttgagt acatgtaatc aaaatacact ttgtaaatta tcatttttat tgaaactcca
     7141 ccatttttct atttataacg ctaataattt gaaaaagaaa cctattgcga accgcggggt
     7201 gaatcccaaa aacgaatgcg ttttggtgga gtgattgatt cgaatcgaag aagaaaaaga
     7261 agaagacgtg gaatagagag ctcactctta accgagcagc acacaccgac agaaaaaaaa
     7321 atgaaatgaa tgagggtctt cttcttcttc ttcttcgaat gattgacaga aatgggaaaa
     7381 agaggaagat tgagaaggga aaaaggaagg agaaaagaag cagaagaaga cgtcagagag
     7441 gagaggaacg agcggaaaag cagcgggcgc aagtcataga agtagcagag ctggggagaa
     7501 gaagacacta tccaagaaag gaatgacgag agagtatgca aaggggtata gggtgcagac
     7561 agaataggaa cagaataaca gatgatgagc caagaagagt tgaaaagggc gatgaatttg
     7621 tcatgtaact taatttgggt caatttgagc atgatgaatt gaaatcatcc cttgttggga
     7681 gttaataacc ggtttgttat cagaaaccct gtaatagaag ggcgccctaa ctttgagcca
     7741 attcatcccg gtttctgtca aatatatcaa aaagtggtca actgacaaat tgtttttgat
     7801 attataataa acattttatc cgttaacaat tttcgaatac tttttacaag gacttggata
     7861 aattggctca aagagcctgc ttttttgtac aaacttgaat tcaaaaacgc agaactcgta
     7921 ctgttggatg cctgtgtagc ggtcctctat tgatttttgc tattttttga taattttact
     7981 gtaaattatg agttattcca attaacattc ctagctaaat gtaagttagc gaccaatttt
     8041 ttagcaaccc cattttatga cttttcagaa tatcgcctac tgggtcaacg acacttgttt
     8101 ggaactaagg gtcttcccaa ttttttttaa atcttcatag gcttgacaga atcagggttc
     8161 ttcaagcagc tttgagaggg aatcaattgc aacatttgat gttaaattta tattttttat
     8221 agttctctgg tgaaatgggg accaccgcaa agcccctttt atgtttttaa cttgaataaa
     8281 atcttgtatt aaattgtgat ttatagatct gtatgggtaa tttgaaggaa aagaaaggga
     8341 gctcccaaaa gtgccttaca gatccaggcc caataaatgt aaactattgg gcagtagtat
     8401 ggcaaaaatc gtgcggaatt tgaaaaacct agaattaaaa taaatattca acacaaatgt
     8461 tcgagatatt ttaagttcgg agtgtatatc tagaacatat tgtacaagta ggtctacggg
     8521 atcgtctacg ggaaaatttg atttcctcga agatgtattt aatgaattct ctaatgttgc
     8581 aggtctttgt atttgacaga atgttggttt ttgaaactta ttcgccataa ttgcttggct
     8641 actacatgtc tcatgccatt gacactatcc tgtactcgaa atttgttaag taactaccca
     8701 ccgtccactt atgacgagag ggtcaattaa ggtcactcca caaaagtaaa tttgaaaaca
     8761 catgagaaaa agtgatctga attcccttga agtggctaaa acaaagcaga tgagggatac
     8821 gacgtatgcg actactgaga gataaggtcg aaacttgata aggatgctgc ttacctgctt
     8881 agcaagtttc ttcgcacagg agaaagtgac aagctaaact atcaaattca tctaaaacct
     8941 caaaaaatta ttttggatga aataatgttt tattttgagc aacgcataag gcaaagaata
     9001 aattaaaggc gatccgctta gcggcgagtt ccgtcctcga agaagattcc tccatcgatg
     9061 gcgcagtact ttggacagat tccacgctct cctggggttc ctggtggtcc gactggtcct
     9121 ggctgtcctg ggtttccgtc ggctcctggc ttgccgtctg gtcctggtgg tccctttggt
     9181 ccaggttgtc ctgaaagatg tggtgattag atatggattg aaaaactatg aaatgattac
     9241 ctggggttcc gtcgtttcct ggttgtccgg atggtcctgg tggtccgact ggtcctggct
     9301 ctccggtgga tcctggctct cccttttgga ctggctcgca gacagctgga agtccctcca
     9361 actttctata caaagttgat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9421 attgttatcc gctggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9481 gggataacgc aggaaagaac atgtgagcaa aaggcca
//
