LOCUS       sequence-169076-        6890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-Sox2-IP
FEATURES             Location/Qualifiers
     source          1..6890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             9..602
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    665..1022
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1081..1497
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1427..1449
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1465..1481
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1507..1881
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1819..1835
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1912..1936
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2897..2921)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(2969..3543,3544..3546,3547..3555)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3136..3153)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3363..3382
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3556..4155
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3556..3575)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4052..4072
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4382..4851
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4981..4998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5152..5740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5232..5251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5904..6695,6696..6764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6527..6546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6765..6869)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctgcataa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
       61 tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca
      121 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
      181 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
      241 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
      301 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
      361 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
      421 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga
      481 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
      541 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
      601 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
      661 aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt
      721 ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg
      781 gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg
      841 gccgtttttg tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca
      901 cccccctaat aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
      961 cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc
     1021 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
     1081 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     1141 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     1201 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     1261 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     1321 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     1381 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     1441 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     1501 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     1561 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     1621 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     1681 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     1741 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     1801 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     1861 ggtggaccat cctctagact gccggatcta gctagttaat taaggatcat cacaagtttg
     1921 tacaaaaaag caggcttgta taacatgatg gagacggagc tgaagccgcc gggcccgcag
     1981 caagcttcgg ggggcggcgg cggaggaggc aacgccacgg cggcggcgac cggcggcaac
     2041 cagaagaaca gcccggaccg cgtcaagagg cccatgaacg ccttcatggt atggtcccgg
     2101 gggcagcggc gtaagatggc ccaggagaac cccaagatgc acaactcgga gatcagcaag
     2161 cgcctgggcg cggagtggaa acttttgtcc gagaccgaga agcggccgtt catcgacgag
     2221 gccaagcggc tgcgcgctct gcacatgaag gagcacccgg attataaata ccggccgcgg
     2281 cggaaaacca agacgctcat gaagaaggat aagtacacgc ttcccggagg cttgctggcc
     2341 cccggcggga acagcatggc gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg
     2401 aaccagcgca tggacagcta cgcgcacatg aacggctgga gcaacggcag ctacagcatg
     2461 atgcaggagc agctgggcta cccgcagcac ccgggcctca acgctcacgg cgcggcacag
     2521 atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt acaactccat gaccagctcg
     2581 cagacctaca tgaacggctc gcccacctac agcatgtcct actcgcagca gggcaccccc
     2641 ggtatggcgc tgggctccat gggctctgtg gtcaagtccg aggccagctc cagccccccc
     2701 gtggttacct cttcctccca ctccagggcg ccctgccagg ccggggacct ccgggacatg
     2761 atcagcatgt acctccccgg cgccgaggtg ccggagcccg ctgcgcccag tagactgcac
     2821 atggcccagc actaccagag cggcccggtg cccggcacgg ccattaacgg cacactgccc
     2881 ctgtcgcaca tgtgaaaccc agctttcttg tacaaagtgg tgattcgagc ggccgctcga
     2941 taagcttgat atcgaattcc gccccccccc cctctccctc ccccccccct aacgttactg
     3001 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     3061 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     3121 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     3181 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     3241 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     3301 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3361 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     3421 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     3481 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3541 aatatggcca caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3601 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3661 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3721 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3781 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3841 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3901 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3961 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4021 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4081 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4141 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     4201 ccccatgcat cgtcgacgat aaaataaaag attttattta gtctccagaa aaagggggga
     4261 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     4321 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     4381 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     4441 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4501 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     4561 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     4621 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     4681 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     4741 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     4801 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
     4861 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
     4921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5821 atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata
     5881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6181 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
//
