LOCUS       sequence-169077-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Jallepalli PV, Waizenegger IC, Bunz F, Langer S, Speicher MR, Peters
            JM, Kinzler KW, Vogelstein B, Lengauer C
  TITLE     Securin is required for chromosomal stability in human cells.
  JOURNAL   Cell. 2001 May 18. 105(4):445-57.
  PUBMED    11371342
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2001 
            May 18. 105(4):445-57."
COMMENT     Sequence Label: pShuttle-Cre-HA
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_signal     2223..2373
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        2672..3051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3052..3255
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3206..3226
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3252..3276
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3297..4325
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4341..4367
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     polyA_signal    4405..4539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4429..4448)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4483..4502
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             8344..8535
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    8637..8777
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8692..8711
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt cttcaagaat tgatccgggc ccccatttcc cctcccttcc
     2041 agctctctgc cccttttgga ttgaagccaa tatgataatg agggggtgga gtttgtgacg
     2101 tggcgcgggg cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt gtgatgttgc
     2161 aagtgtggcg gaacacatgt aagcgacgga tgtggcaaaa gtgacgtttt tggtgtgcgc
     2221 cggtgtacac aggaagtgac aattttcgcg cggttttagg cggatgttgt agtaaatttg
     2281 ggcgtaaccg agtaagattt ggccattttc gcgggaaaac tgaataagag gaagtgaaat
     2341 ctgaataatt ttgtgttact catagcgcgt aatctctagc atcgatgtcg agggatccct
     2401 cgagtctaga gatatcgaat tcaagcttgt cgaccaattc tcatgtttga cagcttatca
     2461 tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag
     2521 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     2581 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     2641 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     2701 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     2761 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     2821 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     2881 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     2941 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     3001 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3061 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3121 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     3181 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     3241 tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt accaccatgt
     3301 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
     3361 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
     3421 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
     3481 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
     3541 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
     3601 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
     3661 aggaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
     3721 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
     3781 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
     3841 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
     3901 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
     3961 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
     4021 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     4081 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     4141 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     4201 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca
     4261 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     4321 gcgattacga tgttccagat tacgcttacg atgttccaga ttacgctagc taaggatagc
     4381 ggccgctcga ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga
     4441 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4501 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4561 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     4621 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     4681 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     4741 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     4801 ggtcgactcg aagatctggg cgtggttaag ggtgggaaag aatatataag gtgggggtct
     4861 tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga
     4921 tggaagcatt gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca
     4981 gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt
     5041 gacctacgag accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc
     5101 cgctgcagcc accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag
     5161 cagtgcagct tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt
     5221 ggattctttg acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca
     5281 ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc
     5341 agactctgtt tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg
     5401 cgcgcggtag gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag
     5461 gacgtggtaa aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg
     5521 gaggtagcac cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta
     5581 gcaggagcgc tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg
     5641 caggcccttg gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga
     5701 tatgagatgc atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg
     5761 gggattcatg ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc
     5821 atgtagctta gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt
     5881 ttccatgcat tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat
     5941 atttctggga tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt
     6001 tacaaagcgc gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc
     6061 gtagttaccc tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc
     6121 tacctgcggg gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag
     6181 caggttcctg agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac
     6241 cggctgcaac tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac
     6301 ttcgttaagc atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc
     6361 gccgcccagc gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc
     6421 cgccgtaggc atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt
     6481 cacctgctct acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt
     6541 tcgctgtacg gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg
     6601 cgcagggtcc tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg
     6661 ctggccaggg tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc
     6721 tgcgcgtcgg ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg
     6781 cccttggcgc gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg
     6841 agggcgtaga gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag
     6901 gccccgcaga cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa
     6961 accaggtttc ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt
     7021 ccacgctcgg tgacgaaaag gctgtccgtg tccccgtata cagacttgag aggcctgtcc
     7081 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     7141 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     7201 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     7261 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
     7321 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     7381 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     7441 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     7501 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     7561 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     7621 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     7681 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     7741 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     7801 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc
     7861 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     7921 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     7981 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     8041 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     8101 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     8161 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     8221 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     8281 ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca
     8341 tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca
     8401 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa
     8461 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac
     8521 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8581 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     8641 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     8701 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8761 gaaaataccg catcaggcgc t
//
