LOCUS       sequence-169080-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing EBFP2 fluorescent protein driven by 
            SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, 
            Lamszus K, Fehse B
  TITLE     Optical Barcoding for Single-Clone Tracking to Study Tumor 
            Heterogeneity.
  JOURNAL   Mol Ther. 2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014.
  PUBMED    28109958
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014."
COMMENT     Sequence Label: LeGO-EBFP2
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(55..57,58..60,61..774)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(794..827)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(936..956)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cggatcccgc caccatggtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgaggggc gagggcgagg gcgatgccac caacggcaag
      181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccctga gccacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      361 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcgt cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact tcaacagcca caacatctat atcatggccg tcaagcagaa gaacggcatc
      541 aaggtgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac
      601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacag ccactacctg
      661 agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      721 gagttccgca ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagaattc
      781 gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg
      841 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
      901 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      961 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggc
//
