LOCUS       sequence-169087-        3921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GNA13, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GNA13_WT
FEATURES             Location/Qualifiers
     source          1..3921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     525..544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     778..795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(954..981)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1073..1159)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1208..1230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1222..1239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1223..1239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1255..1354
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2486..2585)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2602..2621)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2603..2621)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2626..2642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2626..2642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3073..3864
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      901 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      961 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     1021 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     1081 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     1141 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     1201 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     1261 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     1321 tttataatgc caactttgta caaaaaagtt ggcatggcgg acttcctgcc gtcgcggtcc
     1381 gtgctgtccg tgtgcttccc cggctgcctg ctgacgagtg gcgaggccga gcagcaacgc
     1441 aagtccaagg agatcgacaa atgcctgtct cgggaaaaga cctatgtgaa gcggctggtg
     1501 aagatcctgc tgctgggcgc gggcgagagc ggcaagtcca ccttcctgaa gcagatgcgg
     1561 atcatccacg ggcaggactt cgaccagcgc gcgcgcgagg agttccgccc caccatctac
     1621 agcaacgtga tcaaaggtat gagggtgctg gttgatgctc gagagaagct tcatattccc
     1681 tggggagaca actcaaacca acaacatgga gataagatga tgtcgtttga tacccgggcc
     1741 cccatggcag cccaaggaat ggtggaaaca agggttttct tacaatatct tcctgctata
     1801 agagcattat gggcagacag cggcatacag aatgcctatg accggcgtcg agaatttcaa
     1861 ctgggtgaat ctgtaaaata tttcctggat aacttggata aacttggaga accagattat
     1921 attccatcac aacaagatat tctgcttgcc agaagaccca ccaaaggcat ccatgaatac
     1981 gactttgaaa taaaaaatgt tcctttcaaa atggttgatg taggtggtca gagatcagaa
     2041 aggaaacgtt ggtttgaatg tttcgacagt gtgacatcaa tacttttcct tgtttcctca
     2101 agtgaatttg accaggtgct tatggaagat cgactgacca atcgccttac agagtctctg
     2161 aacatttttg aaacaatcgt caataaccgg gttttcagca atgtctccat aattctgttc
     2221 ttaaacaaga cagacttgct tgaggagaag gtgcaaattg tgagcatcaa agactatttc
     2281 ctagaatttg aaggggatcc ccactgctta agagacgtcc aaaaattcct ggtggaatgt
     2341 ttccggaaca aacgccggga ccagcaacag aagcccttat accaccactt caccactgct
     2401 atcaacacgg agaacatccg ccttgttttc cgtgacgtga aggatactat tctgcatgac
     2461 aacctcaagc agcttatgct acagtgccca actttcttgt acaaagttgg cattataaga
     2521 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2581 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2641 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2701 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     2761 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2821 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2881 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2941 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3001 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     3061 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     3121 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     3181 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     3241 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     3301 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     3361 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     3421 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     3481 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3541 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3601 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3661 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3721 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3781 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3841 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     3901 agttacgcgt cgttccactg a
//
