LOCUS       sequence-169089-        6483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAG2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAG2_WT
FEATURES             Location/Qualifiers
     source          1..6483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(943..970)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1062..1148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1197..1219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1211..1228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1212..1228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1244..1343
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5037..5136)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5153..5172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5154..5172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5177..5193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5177..5193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5624..6415
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      901 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
      961 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     1021 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     1081 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     1141 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     1201 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt
     1261 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
     1321 aactttgtac aaaaaagttg gcatgatagc agctccagaa ataccaactg attttaatct
     1381 actacaggag tcagaaacac atttttcttc tgacacagat tttgaagata tcgaaggaaa
     1441 aaaccaaaag caaggcaaag gcaaaacttg taaaaaaggc aaaaagggcc cagcagaaaa
     1501 gggcaaaggt ggaaatggag gaggaaaacc tccttctggt ccaaaccgaa tgaatggtca
     1561 tcaccaacag aatggagtgg aaaacatgat gttgtttgaa gttgttaaaa tgggcaagag
     1621 tgctatgcag tcggtggtag atgattggat agaatcatac aagcatgacc gagatatagc
     1681 acttcttgac cttatcaact tttttattca gtgttcaggc tgtaaaggag ttgtcacagc
     1741 agaaatgttt agacatatgc agaactctga gataattcga aaaatgactg aagaattcga
     1801 tgaggatagt ggagattatc cacttaccat ggctggtcct cagtggaaga agttcaaatc
     1861 cagtttttgt gaattcattg gcgtgttagt acggcaatgt caatatagta tcatatatga
     1921 tgagtatatg atggatacag tcatttcact tcttacagga ttgtctgact cacaagtcag
     1981 agcatttcga catacaagca ccctggcagc tatgaagttg atgacagctt tggtgaatgt
     2041 ggcactaaat cttagcatta atatggataa tacacaaaga caatatgaag cagaacggaa
     2101 taaaatgatt ggaaaacgag ccaatgagag gctagaactc ctgctacaaa agcggaaaga
     2161 gcttcaggaa aatcaagatg aaatagaaaa tatgatgaat gcaatattta aaggagtgtt
     2221 tgtacataga taccgtgatg cgatagctga aattcgagct atttgcattg aagagattgg
     2281 catttggatg aagatgtata gtgatgcctt tcttaatgac agttatttaa aatatgttgg
     2341 ttggactatg catgataagc aaggtgaagt aagactcaaa tgtcttactg ctctacaagg
     2401 gctttattat aacaaagagc ttaattccaa actggaactt tttaccagtc ggttcaagga
     2461 tagaattgtg tctatgaccc ttgacaaaga atatgatgtt gcagtacaag caataaaatt
     2521 actcactctt gttttacaga gtagtgaaga agttctcact gcagaagatt gtgaaaatgt
     2581 ctatcatctg gtttattcag ctcaccggcc agtagcagta gcagctggag aatttctcta
     2641 caaaaagctc ttcagtcgta gagatccaga ggaggatgga atgatgaaaa gaagaggaag
     2701 acaaggtcca aatgccaacc ttgttaagac attggttttt ttctttctag aaagtgagtt
     2761 acatgagcat gcagcatacc ttgtggatag catgtgggac tgtgctactg agctgctgaa
     2821 agactgggaa tgtatgaata gcttgttact ggaagagcca cttagtggag aggaagcact
     2881 aacagatagg caagagagtg ctctgattga aataatgctt tgtaccatta gacaagcggc
     2941 tgaatgtcat cctcccgtgg gaagagggac aggaaaaagg gtgcttacag caaaggagaa
     3001 gaagacacag ttggatgata ggacaaaaat cactgagctt tttgccgtgg cccttcctca
     3061 gttattagca aaatactctg tagatgcaga aaaggtgact aacttgttgc agttgcctca
     3121 gtactttgat ttggaaatat ataccactgg acgattagaa aagcatttgg atgccttatt
     3181 gcgacagatc cggaatattg tagagaagca cacagataca gatgttttgg aagcatgttc
     3241 taaaacttac catgcactct gtaatgaaga gttcacaatc ttcaacagag tagatatttc
     3301 aagaagtcaa ctgatagatg aattggcaga taaatttaac cggcttcttg aagattttct
     3361 gcaagagggt gaagaacctg atgaagatga tgcatatcag gtattgtcaa cattgaagag
     3421 gatcactgct tttcataatg cccatgacct ttcaaagtgg gatttatttg cttgtaatta
     3481 caaactcttg aaaactggaa tcgaaaatgg agacatgcct gagcagattg ttattcacgc
     3541 actgcagtgt actcactatg taatcctttg gcaacttgct aagataactg aaagcagctc
     3601 tacaaaggag gacttgctgc gtttaaagaa acaaatgaga gtattttgtc agatatgtca
     3661 acattacctg accaacgtga atactactgt taaggaacag gccttcacta ttctgtgtga
     3721 tattttgatg atcttcagcc atcagattat gtcaggaggg cgtgacatgt tagagccatt
     3781 agtgtatacc cctgattctt cattgcagtc tgagttgctc agctttattt tggatcatgt
     3841 cttcattgaa caggatgatg ataataatag tgcagatggt cagcaagagg atgaagccag
     3901 taaaattgaa gctctgcaca agagaagaaa tttacttgca gcattttgta agctaattgt
     3961 atatactgtg gtggagatga atacagctgc agatatcttc aaacagtata tgaagtatta
     4021 taatgactat ggagatatca tcaaagaaac aatgagtaaa acaaggcaga tagacaaaat
     4081 tcagtgtgct aagaccctta ttctcagtct gcaacagctt tttaatgaaa tgatacaaga
     4141 aaatggctat aattttgata gatcatcctc tacatttagt ggcataaaag aacttgctcg
     4201 acgttttgct ttaacttttg gacttgatca gttgaaaaca agagaagcca ttgccatgct
     4261 acacaaagat ggcatagaat ttgcttttaa agagcctaat ccgcaagggg agagccatcc
     4321 acctttaaat ttggcatttc ttgatattct gagtgaattt tcttctaaac tacttcgaca
     4381 agacaaaaga acagtgtatg tttacttgga aaagttcatg acctttcaga tgtcactccg
     4441 aagagaggat gtgtggcttc cactgatgtc ttaccgaaat tctttgctag ctggtggtga
     4501 tgatgacacc atgtcagtca ttagtggaat cagcagccgg gggtcaacag tacggagtaa
     4561 aaaatcaaaa ccatctacag gaaaacggaa agtggttgag ggcatgcagc tttcactcac
     4621 tgaagaaagt agtagtagtg acagtatgtg gttaagcaga gaacaaacac tgcacacccc
     4681 tgttatgatg cagacaccac aactcacctc cactattatg agagagccca aaagattacg
     4741 gcctgaggat agcttcatga gtgtttatcc aatgcagact gaacatcatc aaacacctct
     4801 tgattataat cggcgtggca caagcctaat ggaagatgat gaagagccaa ttgtggaaga
     4861 tgttatgatg tcctcagaag ggaggattga ggatcttaat gagggaatgg attttgacac
     4921 catggatata gatttgccac catcaaagaa cagacgagag agaacagaac tgaagcctga
     4981 tttctttgat ccagcttcaa ttatggatga atcagttctt ggagtgtcaa tgttttaccc
     5041 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     5101 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     5161 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     5221 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     5281 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     5341 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     5401 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     5461 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     5521 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     5581 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     5641 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     5701 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     5761 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     5821 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     5881 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     5941 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     6001 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     6061 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     6121 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     6181 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     6241 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     6301 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     6361 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     6421 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     6481 ccc
//
