LOCUS       sequence-169097-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing dKatushka2 fluorescent protein driven 
            by SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, 
            Lamszus K, Fehse B
  TITLE     Optical Barcoding for Single-Clone Tracking to Study Tumor 
            Heterogeneity.
  JOURNAL   Mol Ther. 2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014.
  PUBMED    28109958
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014."
COMMENT     Sequence Label: LeGO-dKatushka2
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(791..824)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(933..953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cggatcccgc caccatggtg
       61 ggcgaggaca gcgtgctgat caccgagaac atgcacatga aactctacat ggagggcacc
      121 gtgaacgacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      181 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      241 gctaccagct tcatgtacgg cagcaaaacc tttatcaacc acacccaggg catccccgac
      301 ttctttaagc agtccttccc tgagggcttc acatgggaga ggatcaccac atacgaagac
      361 gggggcgtgc tgaccgctac ccaggacacc agcctccaga acggctgcct catctacaac
      421 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
      481 ggctgggagg ccagcaccga gatgctgtac cccgctgaca gcggcctgag aggccatgcc
      541 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccctcaa gaccacatac
      601 agatccaaga aacccgctaa gaacctcaag atgcccggct tctacttcgt ggacaggaga
      661 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
      721 gccaggtact gcgacctgcc tagcaaactg gggcacagcc tgtacaagta agaattcgtc
      781 gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc
      841 cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
      901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat t
//
