LOCUS       sequence-169124-        6434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Dai C, Whitesell L, Rogers AB, Lindquist S
  TITLE     Heat shock factor 1 is a powerful multifaceted modifier of 
            carcinogenesis.
  JOURNAL   Cell. 2007 Sep 21. 130(6):1005-18.
  PUBMED    17889646
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Sep 21. 130(6):1005-18."
COMMENT     Sequence Label: pBabe-c-myc-zeo
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1926..2395
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2460..2659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2860..3276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3206..3228
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3244..3260
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3357..4676
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     primer_bind     complement(4957..4977)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4962..5291
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5142..5277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5301..5675
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5846..6315
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
     2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
     2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
     2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac
     3301 gtaggaattc cgatcccagt gtggtggtac agcttgggct gcaggtcgac tctagtatgc
     3361 ccctcaacgt tagcttcacc aacaggaact atgacctcga ctacgactcg gtgcagccgt
     3421 atttctactg cgacgaggag gagaacttct accagcagca gcagcagagc gagctgcagc
     3481 ccccggcgcc cagcgaggat atctggaaga aattcgagct gctgcccacc ccgcccctgt
     3541 cccctagccg ccgctccggg ctctgctcgc cctcctacgt tgcggtcaca cccttctccc
     3601 ttcggggaga caacgacggc ggtggcggga gcttctccac ggccgaccag ctggagatgg
     3661 tgaccgagct gctgggagga gacatggtga accagagttt catctgcgac ccggacgacg
     3721 agaccttcat caaaaacatc atcatccagg actgtatgtg gagcggcttc tcggccgccg
     3781 ccaagctcgt ctcagagaag ctggcctcct accaggctgc gcgcaaagac agcggcagcc
     3841 cgaaccccgc ccgcggccac agcgtctgct ccacctccag cttgtacctg caggatctga
     3901 gcgccgccgc ctcagagtgc atcgacccct cggtggtctt cccctaccct ctcaacgaca
     3961 gcagctcgcc caagtcctgc gcctcgcaag actctagcgc cttctctccg tcctcggatt
     4021 ctctgctctc ctcgacggag tcctccccgc agggcagccc cgagcccctg gtgctccatg
     4081 aggagacacc gcccaccacc agcagcgact ctgaggagga acaagaagat gaggaagaaa
     4141 tcgatgttgt ttctgtggaa aagaggcagg ctcctggcaa aaggtcagag tctggatcac
     4201 cttctgctgg aggccacagc aaacctcctc acagcccact ggtcctcaag aggtgccacg
     4261 tctccacaca tcagcacaac tacgcagcgc ctccctccac tcggaaggac tatcctgctg
     4321 ccaagagggt caagttggac agtgtcagag tcctgagaca gatcagcaac aaccgaaaat
     4381 gcaccagccc caggtcctcg gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct
     4441 tggagcgcca gaggaggaac gagctaaaac ggagcttttt tgccctgcgt gaccagatcc
     4501 cggagttgga aaacaatgaa aaggccccca aggtagttat ccttaaaaaa gccacagcat
     4561 acatcctgtc cgtccaagca gaggagcaaa agctcatttc tgaagaggac ttgttgcgga
     4621 aacgacgaga acagttgaaa cacaaacttg aacagctacg gaactcttgt gcgtaaggaa
     4681 aagtaaggaa aatgattcct tctaacagaa atgtcctgag caatcaccta tgaacttgtt
     4741 tcaaatgcat gatcaaatgc aacctcacaa ccttggctga gtcttgagac tgaaagattt
     4801 agcatctttt ttttttcttt aacagatttg tatttaagaa ttgtttttaa aaaattttaa
     4861 gatttacaca atgtttctct gtaaatattg ccattaaatg taaataactt ccgaattggg
     4921 cgagctcgaa ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg
     4981 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5041 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5101 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5161 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5221 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5281 gcttttgcaa aaagcttacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     5341 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     5401 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     5461 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     5521 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     5581 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     5641 actgcgtgca cttcgtggcc gaggagcagg actgaccgat cgataaaata aaagatttta
     5701 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     5761 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
     5821 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     5881 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     5941 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     6001 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     6061 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     6121 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     6181 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     6241 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     6301 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat
     6361 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6421 gcgtattggg cgct
//
