LOCUS       sequence-169129-        7270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Gupta PB, Kuperwasser C, Brunet JP, Ramaswamy S, Kuo WL, Gray JW, 
            Naber SP, Weinberg RA
  TITLE     The melanocyte differentiation program predisposes to metastasis 
            after neoplastic transformation.
  JOURNAL   Nat Genet. 2005 Oct . 37(10):1047-54.
  PUBMED    16142232
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2005 Oct . 37(10):1047-54."
COMMENT     Sequence Label: pBABE-puro HGF
FEATURES             Location/Qualifiers
     source          1..7270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3326..3337
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3600..3620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3605..3934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3785..3920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3944..4543
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3944..3963)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4440..4460
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4764..5233
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5363..5380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5534..6122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5614..5633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6282..7073,7074..7142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6905..6924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7143..7247)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccgc cagcccgtcc
     1381 agcagcacca tgtgggtgac caaactcctg ccagccctgc tgctgcagca tgtcctcctg
     1441 catctcctcc tgctccccat cgccatcccc tatgcagagg gacaaaggaa aagaagaaat
     1501 acaattcatg aattcaaaaa atcagcaaag actaccctaa tcaaaataga tccagcactg
     1561 aagataaaaa ccaaaaaagt gaatactgca gaccaatgtg ctaatagatg tactaggaat
     1621 aaaggacttc cattcacttg caaggctttt gtttttgata aagcaagaaa acaatgcctc
     1681 tggttcccct tcaatagcat gtcaagtgga gtgaaaaaag aatttggcca tgaatttgac
     1741 ctctatgaaa acaaagacta cattagaaac tgcatcattg gtaaaggacg cagctacaag
     1801 ggaacagtat ctatcactaa gagtggcatc aaatgtcagc cctggagttc catgatacca
     1861 cacgaacaca gctttttgcc ttcgagctat cggggtaaag acctacagga aaactactgt
     1921 cgaaatcctc gaggggaaga agggggaccc tggtgtttca caagcaatcc agaggtacgc
     1981 tacgaagtct gtgacattcc tcagtgttca gaagttgaat gcatgacctg caatggggag
     2041 agttatcgag gtctcatgga tcatacagaa tcaggcaaga tttgtcagcg ctgggatcat
     2101 cagacaccac accggcacaa attcttgcct gaaagatatc ccgacaaggg ctttgatgat
     2161 aattattgcc gcaatcccga tggccagccg aggccatggt gctatactct tgaccctcac
     2221 acccgctggg agtactgtgc aattaaaaca tgcgctgaca atactatgaa tgacactgat
     2281 gttcctttgg aaacaactga atgcatccaa ggtcaaggag aaggctacag gggcactgtc
     2341 aataccattt ggaatggaat tccatgtcag cgttgggatt ctcagtatcc tcacgagcat
     2401 gacatgactc ctgaaaattt caagtgcaag gacctacgag aaaattactg ccgaaatcca
     2461 gatgggtctg aatcaccctg gtgttttacc actgatccaa acatccgagt tggctactgc
     2521 tcccaaattc caaactgtga tatgtcacat ggacaagatt gttatcgtgg gaatggcaaa
     2581 aattatatgg gcaacttatc ccaaacaaga tctggactaa catgttcaat gtgggacaag
     2641 aacatggaag acttacatcg tcatatcttc tgggaaccag atgcaagtaa gctgaatgag
     2701 aattactgcc gaaatccaga tgatgatgct catggaccct ggtgctacac gggaaatcca
     2761 ctcattcctt gggattattg ccctatttct cgttgtgaag gtgataccac acctacaata
     2821 gtcaatttag accatcccgt aatatcttgt gccaaaacga aacaattgcg agttgtaaat
     2881 gggattccaa cacgaacaaa cataggatgg atggttagtt tgagatacag aaataaacat
     2941 atctgcggag gatcattgat aaaggagagt tgggttctta ctgcacgaca gtgtttccct
     3001 tctcgagact tgaaagatta tgaagcttgg cttggaattc atgatgtcca cggaagagga
     3061 gatgagaaat gcaaacaggt tctcaatgtt tcccagctgg tatatggccc tgaaggatca
     3121 gatctggttt taatgaagct tgccaggcct gctgtcctgg atgattttgt tagtacgatt
     3181 gatttaccta attatggatg cacaattcct gaaaagacca gttgcagtgt ttatggctgg
     3241 ggctacactg gattgatcaa ctatgatggc ctattacgag tggcacatct ctatataatg
     3301 ggaaatgaga aatgcagcca gcatcatcga gggaaggtga ctctgaatga gtctgaaata
     3361 tgtgctgggg ctgaaaagat tggatcagga ccatgtgagg gggattatgg tggcccactt
     3421 gtttgtgagc aacataaaat gagaatggtt cttggtgtca ttgttcctgg tcgtggatgt
     3481 gccattccaa atcgtcctgg tatttttgtc cgagtagcat attatgcaaa atggatacac
     3541 aaaattattt taacatataa ggtaccacag tcatagctgt taacccgggt cgaccctgtg
     3601 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3661 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3841 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3901 aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg agtacaagcc
     3961 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     4021 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4081 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4141 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4201 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4261 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4321 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4381 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4441 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4501 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     4561 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt
     4621 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     4681 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
     4741 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     4801 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     4861 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     4921 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     4981 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     5041 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
     5101 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     5161 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     5221 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     5281 acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5341 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5941 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgc
     6241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7261 aaagtgccac
//
