LOCUS       sequence-169132-        4914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FBXW7, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FBXW7_WT
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     527..546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     780..797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(956..983)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1075..1161)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1210..1232
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1224..1241
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1225..1241
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1257..1356
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3481..3580)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3597..3616)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3598..3616)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3621..3637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3621..3637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4068..4859
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
       61 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      901 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
      961 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     1021 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     1081 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     1141 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     1201 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     1261 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1321 tttttataat gccaactttg tacaaaaaag ttggcaccat gaatcaggaa ctgctctctg
     1381 tgggcagcaa aagacgacga actggaggct ctctgagagg taacccttcc tcaagccagg
     1441 tagatgaaga acagatgaat cgtgtggtag aggaggaaca gcaacagcaa ctcagacaac
     1501 aagaggagga gcacactgca aggaatggtg aagttgttgg agtagaacct agacctggag
     1561 gccaaaatga ttcccagcaa ggacagttgg aagaaaacaa taatagattt atttcggtag
     1621 atgaggactc ctcaggaaac caagaagaac aagaggaaga tgaagaacat gctggtgaac
     1681 aagatgagga ggatgaggag gaggaggaga tggaccagga gagtgacgat tttgatcagt
     1741 ctgatgatag tagcagagaa gatgaacata cacatactaa cagtgtcacg aactccagta
     1801 gtattgtgga cctgcccgtt caccaactct cctccccatt ctatacaaaa acaacaaaaa
     1861 tgaaaagaaa gttggaccat ggttctgagg tccgctcttt ttctttggga aagaaaccat
     1921 gcaaagtctc agaatataca agtaccactg ggcttgtacc atgttcagca acaccaacaa
     1981 cttttgggga cctcagagca gccaatggcc aagggcaaca acgacgccga attacatctg
     2041 tccagccacc tacaggcctc caggaatggc taaaaatgtt tcagagctgg agtggaccag
     2101 agaaattgct tgctttagat gaactcattg atagttgtga accaacacaa gtaaaacata
     2161 tgatgcaagt gatagaaccc cagtttcaac gagacttcat ttcattgctc cctaaagagt
     2221 tggcactcta tgtgctttca ttcctggaac ccaaagacct gctacaagca gctcagacat
     2281 gtcgctactg gagaattttg gctgaagaca accttctctg gagagagaaa tgcaaagaag
     2341 aggggattga tgaaccattg cacatcaaga gaagaaaagt aataaaacca ggtttcatac
     2401 acagtccatg gaaaagtgca tacatcagac agcacagaat tgatactaac tggaggcgag
     2461 gagaactcaa atctcctaag gtgctgaaag gacatgatga tcatgtgatc acatgcttac
     2521 agttttgtgg taaccgaata gttagtggtt ctgatgacaa cactttaaaa gtttggtcag
     2581 cagtcacagg caaatgtctg agaacattag tgggacatac aggtggagta tggtcatcac
     2641 aaatgagaga caacatcatc attagtggat ctacagatcg gacactcaaa gtgtggaatg
     2701 cagagactgg agaatgtata cacaccttat atgggcatac ttccactgtg cgttgtatgc
     2761 atcttcatga aaaaagagtt gttagcggtt ctcgagatgc cactcttagg gtttgggata
     2821 ttgagacagg ccagtgttta catgttttga tgggtcatgt tgcagcagtc cgctgtgttc
     2881 aatatgatgg caggagggtt gttagtggag catatgattt tatggtaaag gtgtgggatc
     2941 cagagactga aacctgtcta cacacgttgc aggggcatac taatagagtc tattcattac
     3001 agtttgatgg tatccatgtg gtgagtggat ctcttgatac atcaatccgt gtttgggatg
     3061 tggagacagg gaattgcatt cacacgttaa cagggcacca gtcgttaaca agtggaatgg
     3121 aactcaaaga caatattctt gtctctggga atgcagattc tacagttaaa atctgggata
     3181 tcaaaacagg acagtgttta caaacattgc aaggtcccaa caagcatcag agtgctgtga
     3241 cctgtttaca gttcaacaag aactttgtaa ttaccagctc agatgatgga actgtaaaac
     3301 tatgggactt gaaaacgggt gaatttattc gaaacctagt cacattggag agtgggggga
     3361 gtgggggagt tgtgtggcgg atcagagcct caaacacaaa gctggtgtgt gcagttggga
     3421 gtcggaatgg gactgaagaa accaagctgc tggtgctgga ctttgatgtg gacatgaagt
     3481 tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     3541 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     3601 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     3661 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     3721 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     3781 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     3841 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     3901 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     3961 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     4021 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     4081 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     4141 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     4201 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     4261 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     4321 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     4381 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     4441 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     4501 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     4561 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     4621 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     4681 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     4741 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     4801 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     4861 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cact
//
