LOCUS       sequence-169144-        3924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SMARCB1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SMARCB1_WT
FEATURES             Location/Qualifiers
     source          1..3924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1803..1902)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1919..1938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1920..1938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1943..1959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1943..1959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2390..3181
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3274..3862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3763..3782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgatgatga tggcgctgag caagaccttc gggcagaagc ccgtgaagtt
      721 ccagctggag gacgacggcg agttctacat gatcggctcc gaggtgggaa actacctccg
      781 tatgttccga ggttctctgt acaagagata cccctcactc tggaggcgac tagccactgt
      841 ggaagagagg aagaaaatag ttgcatcgtc acatgatcac ggatacacga ctctagccac
      901 cagtgtgacc ctgttaaaag cctcggaagt ggaagagatt ctggatggca acgatgagaa
      961 gtacaaggct gtgtccatca gcacagagcc ccccacctac ctcagggaac agaaggccaa
     1021 gaggaacagc cagtgggtac ccaccctgcc caacagctcc caccacttag atgccgtgcc
     1081 atgctccaca accatcaaca ggaaccgcat gggccgagac aagaagagaa ccttccccct
     1141 ttgctttgat gaccatgacc cagctgtgat ccatgagaac gcatctcagc ccgaggtgct
     1201 ggtccccatc cggctggaca tggagatcga tgggcagaag ctgcgagacg ccttcacctg
     1261 gaacatgaat gagaagttga tgacgcctga gatgttttca gaaatcctct gtgacgatct
     1321 ggatttgaac ccgctgacgt ttgtgccagc catcgcctct gccatcagac agcagatcga
     1381 gtcctacccc acggacagca tcctggagga ccagtcagac cagcgcgtca tcatcaagct
     1441 gaacatccat gtgggaaaca tttccctggt ggaccagttt gagtgggaca tgtcagagaa
     1501 ggagaactca ccagagaagt ttgccctgaa gctgtgctcg gagctggggt tgggcgggga
     1561 gtttgtcacc accatcgcat acagcatccg gggacagctg agctggcatc agaagaccta
     1621 cgccttcagc gagaaccctc tgcccacagt ggagattgcc atccggaaca cgggcgatgc
     1681 ggaccagtgg tgcccactgc tggagactct gacagacgct gagatggaga agaagatccg
     1741 cgaccaggac aggaacacga ggcggatgag gcgtcttgcc aacacggccc cggcctggtg
     1801 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1861 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1921 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1981 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     2041 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     2101 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     2161 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2221 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2281 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2341 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     2401 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2461 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2521 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2581 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2641 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2701 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2761 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2821 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2881 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2941 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3001 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3061 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3121 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3181 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3541 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3601 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3661 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3721 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3781 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3841 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3901 ttgctggcct tttgctcaca tgtt
//
