LOCUS       sequence-169145-        4584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing UNC93B1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_UNC93B1_WT
FEATURES             Location/Qualifiers
     source          1..4584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     533..552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     786..803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(962..989)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1081..1167)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1216..1238
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1230..1247
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1231..1247
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1263..1362
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3157..3256)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3273..3292)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3274..3292)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3297..3313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3297..3313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3744..4535
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
       61 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      901 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
      961 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     1021 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     1081 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     1141 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
     1201 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
     1261 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
     1321 aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatggag gcggagccgc
     1381 cgctctaccc gatggcgggg gctgcggggc cgcagggcga cgaggacctg ctcggggtcc
     1441 cggacgggcc cgaggccccg ctggacgagc tggtgggcgc gtaccccaac tacaacgagg
     1501 aggaggagga gcgccgctac taccgccgca agcgcctggg cgtgctcaag aacgtgctgg
     1561 ctgccagcgc cgggggcatg ctcacctacg gcgtctacct gggcctcctg cagatgcagc
     1621 tgatcctgca ctacgacgag acctaccgcg aggtgaagta tggcaacatg gggctgcccg
     1681 acatcgacag caaaatgctg atgggcatca acgtgactcc catcgccgcc ctgctctaca
     1741 cacctgtgct catcaggttt tttggaacga agtggatgat gttcctcgct gtgggcatct
     1801 acgccctctt tgtctccacc aactactggg agcgctacta cacgcttgtg ccctcggctg
     1861 tggccctggg catggccatc gtgcctcttt gggcttccat gggcaactac atcaccagga
     1921 tggcgcagaa gtaccatgag tactcccact acaaggagca ggatgggcag gggatgaagc
     1981 agcggcctcc gcggggctcc cacgcgccct atctcctggt cttccaagcc atcttctaca
     2041 gcttcttcca tctgagcttc gcctgcgccc agctgcccat gatttatttc ctgaaccact
     2101 acctgtatga cctgaaccac acgctgtaca atgtgcagag ctgcggcacc aacagccacg
     2161 ggatcctcag cggcttcaac aagacggttc tgcggacgct cccgcggagc ggaaacctca
     2221 ttgtggtgga gagcgtgctc atggcagtgg ccttcctggc catgctgctg gtgctgggtt
     2281 tgtgcggagc cgcttaccgg cccacggagg agatcgatct gcgcagcgtg ggctggggca
     2341 acatcttcca gctgcccttc aagcacgtgc gtgactaccg cctgcgccac ctcgtgcctt
     2401 tctttatcta cagcggcttc gaggtgctct ttgcctgcac tggtatcgcc ttgggctatg
     2461 gcgtgtgctc ggtggggctg gagcggctgg cttacctcct cgtggcttac agcctgggcg
     2521 cctcagccgc ctcactcctg ggcctgctgg gcctgtggct gccacgcccg gtgcccctgg
     2581 tggccggagc aggggtgcac ctgctgctca ccttcatcct ctttttctgg gcccctgtgc
     2641 ctcgggtcct gcaacacagc tggatcctct atgtggcagc tgccctttgg ggtgtgggca
     2701 gtgccctgaa caagactgga ctcagcacac tcctgggaat cttgtacgaa gacaaggaga
     2761 gacaggactt catcttcacc atctaccact ggtggcaggc tgtggccatc ttcaccgtgt
     2821 acctgggctc gagcctgcac atgaaggcta agctggcggt gctgctggtg acgctggtgg
     2881 cggccgcggt ctcctacctg cggatggagc agaagctgcg ccggggcgtg gccccgcgcc
     2941 agccccgcat cccgcggccc cagcacaagg tgcgcggtta ccgctacttg gaggaggaca
     3001 actcggacga gagcgacgcg gagggcgagc atggggacgg cgcggaggag gaggcgccgc
     3061 ccgcagggcc caggcctggc cccgagcccg ctggactcgg ccgccggccc tgcccgtacg
     3121 aacaggcgca ggggggagac gggccggagg agcagttgcc aactttcttg tacaaagttg
     3181 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     3241 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     3301 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     3361 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     3421 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     3481 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     3541 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     3601 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3661 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     3721 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     3781 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3841 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3901 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3961 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     4021 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     4081 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     4141 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     4201 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     4261 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4321 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4381 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4441 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     4501 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     4561 cccttaacgt gagttacgcg tcgt
//
