LOCUS       sequence-169158-        4146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IDH2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH2_WT
FEATURES             Location/Qualifiers
     source          1..4146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2025..2124)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2141..2160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2142..2160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2165..2181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2165..2181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2612..3403
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3496..4084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3985..4004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gccggctacc tgcgggtcgt gcgctcgctc tgcagagcct caggctcgcg
      721 gccggcctgg gcgccggcgg ccctgacagc ccccacctcg caagagcagc cgcggcgcca
      781 ctatgccgac aaaaggatca aggtggcgaa gcccgtggtg gagatggatg gtgatgagat
      841 gacccgtatt atctggcagt tcatcaagga gaagctcatc ctgccccacg tggacatcca
      901 gctaaagtat tttgacctcg ggctcccaaa ccgtgaccag actgatgacc aggtcaccat
      961 tgactctgca ctggccaccc agaagtacag tgtggctgtc aagtgtgcca ccatcacccc
     1021 tgatgaggcc cgtgtggaag agttcaagct gaagaagatg tggaaaagtc ccaatggaac
     1081 tatccggaac atcctggggg ggactgtctt ccgggagccc atcatctgca aaaacatccc
     1141 acgcctagtc cctggctgga ccaagcccat caccattggc aggcacgccc atggcgacca
     1201 gtacaaggcc acagactttg tggcagaccg ggccggcact ttcaaaatgg tcttcacccc
     1261 aaaagatggc agtggtgtca aggagtggga agtgtacaac ttccccgcag gcggcgtggg
     1321 catgggcatg tacaacaccg acgagtccat ctcaggtttt gcgcacagct gcttccagta
     1381 tgccatccag aagaaatggc cgctgtacat gagcaccaag aacaccatac tgaaagccta
     1441 cgatgggcgt ttcaaggaca tcttccagga gatctttgac aagcactata agaccgactt
     1501 cgacaagaat aagatctggt atgagcaccg gctcattgat gacatggtgg ctcaggtcct
     1561 caagtcttcg ggtggctttg tgtgggcctg caagaactat gacggagatg tgcagtcaga
     1621 catcctggcc cagggctttg gctcccttgg cctgatgacg tccgtcctgg tctgccctga
     1681 tgggaagacg attgaggctg aggccgctca tgggaccgtc acccgccact atcgggagca
     1741 ccagaagggc cggcccacca gcaccaaccc catcgccagc atctttgcct ggacacgtgg
     1801 cctggagcac cgggggaagc tggatgggaa ccaagacctc atcaggtttg cccagatgct
     1861 ggagaaggtg tgcgtggaga cggtggagag tggagccatg accaaggacc tggcgggctg
     1921 cattcacggc ctcagcaatg tgaagctgaa cgagcacttc ctgaacacca cggacttcct
     1981 cgacaccatc aagagcaacc tggacagagc cctgggcagg cagtacccaa ctttcttgta
     2041 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2101 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2161 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2221 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     2281 gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     2341 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     2401 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     2461 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     2521 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     2581 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
     2641 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     2701 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     2761 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     2821 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     2881 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     2941 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     3001 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     3061 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     3121 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     3181 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     3241 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     3301 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     3361 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     3421 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3481 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3601 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4081 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4141 catgtt
//
