LOCUS       sequence-169161-        6285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RIMS2 ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RIMS2_WT
FEATURES             Location/Qualifiers
     source          1..6285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2209..2220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4164..4263)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4280..4299)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4281..4299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4304..4320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4304..4320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4751..5542
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5635..6223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6124..6143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgcaatttg agacattgcg ccaggtctgc aattctgttt tatctcattt
      721 tcatggggtt ttttcatccc caccaaatat cttacaaaat gagctttttg gacaaacact
      781 gaacaatgca aggaaaagaa gcccatctgt gtccagagat cagaatagaa gatacgacca
      841 aagggaagaa agagaggaat attcacagta tgctacttcg gataccgcaa tgcctagatc
      901 tccatcagat tatgctgata ggcgatttca acatgaacct cagttttatg aagactctga
      961 tcatttaagt tatagggact ccaacaggag aagtcatagg cattccaaag aatatattgt
     1021 agatgatgag gatgtggaaa gcagagatga atacgaaagg caaaggagag aggaagagta
     1081 ccagtcacgc taccgaagtg atccgaattt ggcccgttat ccagtaaagc cacaacccta
     1141 tgaagaacaa atgcggatcc atgctgaagt gtcccgagca cggcatgaga gaaggcatag
     1201 tgatgtttct ttggcaaatg ctgatctgga agattccagg atttctatgc taaggatgga
     1261 tcgaccatca aggcaaagat ctatatcaga acgtagagct gccatggaaa atcagcgatc
     1321 ttattcaatg gaaagaactc gagaggctca gggaccaagt tcttatgcac aaaggaccac
     1381 aaaccatagt cctcctaccc ccaggaggag tccactaccc atagatagac cagacttgag
     1441 gcgtactgac tcactacgga aacagcacca cttagatcct agctctgctg taagaaaaac
     1501 aaaacgggaa aaaatggaaa caatgttaag gaatgattct ctcagttcag accggtcaga
     1561 gtcagtgaga cctccaccac caaagcctca taaatcaaag aaaggcggta aaatgcgcca
     1621 gatttcgttg agcagttcag aggaggaatt ggcttccacg cctgaatata caagttgtga
     1681 tgatgttgag attgaaagtg agagtgtaag tgaaaaagga gacatggatt acaactggtt
     1741 ggatcatacg tcttggcata gcagtgaggc atccccaatg tctttgcacc ctgtaacctg
     1801 gcaaccatct aaagatggag atcgtttaat tggtcgcatt ttattaaata agcgtctaaa
     1861 agatggaagt gtacctcgag attcaggagc aatgcttggc ttgaaggttg taggaggaaa
     1921 gatgactgaa tcaggtcggc tttgtgcatt tattactaaa gtaaaaaaag gaagtttagc
     1981 tgatactgta ggacatctta gaccaggtga tgaagtatta gaatggaatg gaagactact
     2041 gcaaggagcc acatttgagg aagtgtacaa catcattcta gaatccaaac ctgaaccaca
     2101 agtagaactt gtagtttcaa ggcctattgg agatataccg cgaatacctg atagcacaca
     2161 tgcacaactg gagtccagtt ctagctcctt tgaatctcaa aaaatggatc gtccttctat
     2221 ttctgttacc tctcccatga gtcctggaat gttgagggat gtcccacagt tcttatcagg
     2281 acaactttca ataaaactat ggtttgacaa ggttggtcac caattaatag ttacaatttt
     2341 gggagcaaaa gatctccctt ccagggaaga tgggaggcca aggaatcctt atgttaaaat
     2401 ttactttctt ccagacagaa gtgataaaaa caagagaaga actaaaacag taaagaaaac
     2461 attggaaccc aaatggaacc aaacattcat ttattctcca gtccaccgaa gagaatttcg
     2521 ggaacgaatg ctagagatta ccctttggga tcaagctcgt gttcgagagg aagaaagtga
     2581 attcttaggg gagattttaa ttgaattaga aacagcatta ttagatgatg agccacattg
     2641 gtacaaactt cagacgcatg atgtctcttc attgccactt ccccaccctt ctccatatat
     2701 gccacgaaga cagctccatg gagagagccc aacacggagg ttgcaaaggt caaagagaat
     2761 aagtgatagt gaagtctctg actatgactg tgatgatgga attggtgtag tatcagatta
     2821 tcgacatgat ggtcgagatc ttcaaagctc aacattatca gtgccagaac aagtaatgtc
     2881 atcaaaccac tgttcaccat cagggtctcc tcatcgagta gatgttatag gaaggactag
     2941 atcatggtca cccagtgtcc ctcctccaca aagtcggaat gtggaacagg ggcttcgagg
     3001 gacccgcact atgaccggac attataatac aattagccga atggacagac atcgtgtcat
     3061 ggatgaccat tattctccag atagagacag tcattttctt actctacctc gctccagata
     3121 cagtcagacc attgaccatc atcacaggga tggcagggat tgtgaagcag cagatagaca
     3181 gccatatcac agatccagat caacagaaca acggcctctc cttgagcgga ccaccacccg
     3241 ctccagatcc actgaacgtc ctgatacaaa cctcatgagg tcgatgcctt cattaatgac
     3301 tggaagatct gcccctcctt cacctgcctt atcgaggtct catcctcgta ctgggtctgt
     3361 ccagacaagc ccatcaagta ctccagtcgc aggacgaagg ggccgacagc ttccacagct
     3421 tccaccaaag ggaacgttgg atagaaaagc aggaggtaaa aaactaagga gcactgtcca
     3481 aagaagtaca gaaacaggcc tggccgtgga aatgaggaac tggatgactc gacaggcaag
     3541 ccgagagtct acagatggta gcatgaacag ctacagctca gaaggaaatc tgattttccc
     3601 tggtgttcgc ttggcctctg atagccagtt cagtgatttc ctggatggcc ttggccctgc
     3661 tcagctagtg ggacgccaga ctctggcaac acctgcaatg ggtgacattc aggtaggaat
     3721 gatggacaaa aagggacagc tggaggtaga aatcatccgg gcccgtggcc ttgttgtaaa
     3781 accaggttcc aagacactgc cagcaccgta tgtaaaagtg tatctattag ataacggagt
     3841 ctgcatagcc aaaaagaaaa caaaagtggc aagaaaaacg ctggaacccc tttaccagca
     3901 gctattatct ttcgaagaga gtccacaagg aaaagtttta cagatcatcg tctggggaga
     3961 ttatggccgc atggatcaca aatcttttat gggagtggcc cagatacttt tagatgaact
     4021 agagctatcc aatatggtga tcggatggtt caaacttttc ccaccttcct ccctagtaga
     4081 tccaaccttg gcccctctga caagaagagc ttcccaatca tctctggaaa gttcaactgg
     4141 accttcttac tctcgttcat gattgccaac tttcttgtac aaagttggca ttataagaaa
     4201 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     4261 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     4321 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     4381 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     4441 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     4501 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     4561 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     4621 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     4681 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     4741 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     4801 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     4861 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     4921 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     4981 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     5041 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     5101 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     5161 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     5221 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     5281 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     5341 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     5401 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     5461 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     5521 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     5581 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5641 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5701 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5761 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5821 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5881 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5941 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6001 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6061 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6121 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6181 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6241 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
