LOCUS       sequence-169168-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hybrid reporter construct with 4 transcription factor binding sites 
            (2 UAS, 2 TetO) driving EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: pU2T2-YFP
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     115..139
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(247..249,250..252,253..765)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(292..313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(553..572)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcaagggtc cccaaactca caccggtcgg aggacagtac tccgcggagg acagtactcc
       61 gtctctatca ctgataggga tctctatcac tgatagggag gtacctataa gcagagctcg
      121 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      181 acaccgggac cgatccagcc tgcctgaagt tctcagctct agagatatcg tcgaggccgc
      241 gctagtatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      301 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      361 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      421 cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac
      481 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      541 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      601 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      661 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      721 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      781 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      841 aaccactacc tgagctacca gt
//
