LOCUS       sequence-169172-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hybrid reporter construct with 4 transcription factor binding sites 
            (2 TetO, 2 UAS) driving EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: pT2U2-YFP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     116..140
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(248..250,251..253,254..766)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(293..314)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(554..573)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcaagggtc cccaaactca caccggttct ctatcactga tagggatctc tatcactgat
       61 agggacggag gacagtactc cgcggaggac agtactccgg gtaccctata agcagagctc
      121 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      181 gacaccggga ccgatccagc ctgcctgaag ttctcagctc tagagatatc gtcgaggccg
      241 cgctagtatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      361 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      421 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
      481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      721 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      841 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      901 catggtcctg ctgga
//
