LOCUS       sequence-169184-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hybrid reporter construct with 6 transcription factor binding sites 
            (2 TetO, 2 UAS, 2 TetO) driving EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: pT2U2T2-YFP
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(18..731,732..734,735..737))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(63..84)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     412..431
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     671..692
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(845..869)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 actaggtcga caagctttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
       61 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      121 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      181 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      241 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
      301 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      361 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      421 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      481 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag
      541 ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      601 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
      661 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      721 tcgcccttgc tcaccatact agcgcggcct cgacgatatc tctagagctg agaacttcag
      781 gcaggctgga tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc
      841 aggcgatctg acggttcact aaacgagctc tgct
//
