LOCUS       sequence-169191-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hybrid reporter construct with 8 transcription factor binding sites 
            (2 UAS, 4 TetO, 2 UAS) driving EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: pU2T4U2-YFP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..77
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    103..121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    122..140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     191..215
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(323..325,326..328,329..841)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(368..389)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(629..648)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagggtcccc aaactcacac cggtcggagg acagtactcc gcggaggaca gtactccgtc
       61 tctatcactg atagggatct ctatcactga tagggagccg gttctctatc actgataggg
      121 atctctatca ctgataggga cggaggacag tactccgcgg aggacagtac tccgggtacc
      181 ctataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      241 ttgacctcca tagaagacac cgggaccgat ccagcctgcc tgaagttctc agctctagag
      301 atatcgtcga ggccgcgcta gtatggtgag caagggcgag gagctgttca ccggggtggt
      361 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      421 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      481 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc
      541 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      601 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      661 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      721 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      781 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      841 ggac
//
